PDE4 (PDE4B) (NM_001037340) Human Untagged Clone

CAT#: SC302894

PDE4B (untagged)-Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE4B
• Synonyms: DPDE4; PDEIVB
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001037340, the custom clone sequence may differ by one or more nucleotides
  ATGACAGCAAAAGATTCTTCAAAGGAACTTACTGCTTCTGAACCTGAGGTTTGCATAAAG
  ACTTTCAAGGAGCAAATGCATTTAGAACTTGAGCTTCCGAGATTACCAGGAAACAGACCT
  ACATCTCCTAAAATTTCTCCACGCAGTTCACCAAGGAACTCACCATGCTTTTTCAGAAAG
  TTGCTGGTGAATAAAAGCATTCGGCAGCGTCGTCGCTTCACTGTGGCTCATACATGCTTT
  GATGTGGAAAATGGCCCTTCCCCAGGTCGGAGTCCACTGGATCCCCAGGCCAGCTCTTCC
  GCTGGGCTGGTACTTCACGCCACCTTTCCTGGGCACAGCCAGCGCAGAGAGTCATTTCTC
  TACAGATCAGACAGCGACTATGACTTGTCACCAAAGGCGATGTCGAGAAACTCTTCTCTT
  CCAAGCGAGCAACACGGCGATGACTTGATTGTAACTCCTTTTGCCCAGGTCCTTGCCAGC
  TTGCGAAGTGTGAGAAACAACTTCACTATACTGACAAACCTTCATGGTACATCTAACAAG
  AGGTCCCCAGCTGCTAGTCAGCCTCCTGTCTCCAGAGTCAACCCACAAGAAGAATCTTAT
  CAAAAATTAGCAATGGAAACGCTGGAGGAATTAGACTGGTGTTTAGACCAGCTAGAGACC
  ATACAGACCTACCGGTCTGTCAGTGAGATGGCTTCTAACAAGTTCAAAAGAATGCTGAAC
  CGGGAGCTGACACACCTCTCAGAGATGAGCCGATCAGGGAACCAGGTGTCTGAATACATT
  TCAAATACTTTCTTAGACAAGCAGAATGATGTGGAGATCCCATCTCCTACCCAGAAAGAC
  AGGGAGAAAAAGAAAAAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAGAAATTAATG
  CATAGTTCAAGCCTAAACAATACAAGCATCTCACGCTTTGGAGTCAACACTGAAAATGAA
  GATCACCTGGCCAAGGAGCTGGAAGACCTGAACAAATGGGGTCTTAACATCTTTAATGTG
  GCTGGATATTCTCACAATAGACCCCTAACATGCATCATGTATGCTATATTCCAGGAAAGA
  GACCTCCTAAAGACATTCAGAATCTCATCTGACACATTTATAACCTACATGATGACTTTA
  GAAGACCATTACCATTCTGACGTGGCATATCACAACAGCCTGCACGCTGCTGATGTAGCC
  CAGTCGACCCATGTTCTCCTTTCTACACCAGCATTAGACGCTGTCTTCACAGATTTGGAG
  ATCCTGGCTGCCATTTTTGCAGCTGCCATCCATGACGTTGATCATCCTGGAGTCTCCAAT
  CAGTTTCTCATCAACACAAATTCAGAACTTGCTTTGATGTATAATGATGAATCTGTGTTG
  GAAAATCATCACCTTGCTGTGGGTTTCAAACTGCTGCAAGAAGAACACTGTGACATCTTC
  ATGAATCTCACCAAGAAGCAGCGTCAGACACTCAGGAAGATGGTTATTGACATGGTGTTA
  GCAACTGATATGTCTAAACATATGAGCCTGCTGGCAGACCTGAAGACAATGGTAGAAACG
  AAGAAAGTTACAAGTTCAGGCGTTCTTCTCCTAGACAACTATACCGATCGCATTCAGGTC
  CTTCGCAACATGGTACACTGTGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTAT
  CGGCAATGGACAGACCGCATCATGGAGGAATTTTTCCAGCAGGGAGACAAAGAGCGGGAG
  AGGGGAATGGAAATTAGCCCAATGTGTGATAAACACACAGCTTCTGTGGAAAAATCCCAG
  GTTGGTTTCATCGACTACATTGTCCATCCATTGTGGGAGACATGGGCAGATTTGGTACAG
  CCTGATGCTCAGGACATTCTCGATACCTTAGAAGATAACAGGAACTGGTATCAGAGCATG
  ATACCTCAAAGTCCCTCACCACCACTGGACGAGCAGAACAGGGACTGCCAGGGTCTGATG
  GAGAAGTTTCAGTTTGAACTGACTCTCGATGAGGAAGATTCTGAAGGACCTGAGAAGGAG
  GGAGAGGGACACAGCTATTTCAGCAGCACAAAGACGCTTTGTGTGATTGATCCAGAAAAC
  AGAGATTCCCTGGGAGAGACTGACATAGACATTGCAACAGAAGACAAGTCCCCCGTGGAT
  ACATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001037340
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037340.1, NP_001032417.1
• RefSeq Size: 4273 bp
• RefSeq ORF: 2166 bp
• Locus ID: 5142
• Cytogenetics: 1p31.3
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (c) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant a. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (3, also known as PDE4B3) has a shorter and distinct N-terminus when compared to isoform 1.