Galactosylceramidase (GALC) (NM_001037525) Human Untagged Clone

CAT#: SC302906

GALC (untagged)-Human galactosylceramidase (GALC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALC
• Synonyms: galactocerebrosidase; galactocerebroside beta-galactosidase; galactosylceramidase; galactosylceramide beta-galactosidase; galactosylceraminidase; GALCERase; OTTHUMP00000027906
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001037525, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGTGGCTACTCTCGGCTTCCTGGCAACGCCGAGCGAAAGCTATGACTGCGGCCGCGGGTTCGG
  CGGGCCGCGCCGCGGTGCCCTTGCTGCTGTGTGCGCTGCTGGCGCCCGGCGGCGCGTACGTGCTCGACGA
  CTCCGACGGGCTGGGCCGGGAGTTCGACGGCATCGGCGCGGTCAGCGGCGGCGGGGCAACCTCCCGACTT
  CTAGTAAATTACCCAGAGCCCTATCGTTCTCAGATATTGGATTATCTCTTTAAGCCGAATTTTGGTGCCT
  CTTTGCATATTTTAAAAGTGGAAATAGGTGGTGATGGGCAGACAACAGACGGCACTGAGCCCTCCCACAT
  GCATTATGCACTAGATGAGAATTATTTCCGAGGATACGAGTGGTGGTTGATGAAAGAAGCTAAGAAGAGG
  AATCCCAATATTACACTCATTGGGTTGCCATGGTCATTCCCTGGATGGCTGGGAAAAGGTTTCGACTGGC
  CTTATGTCAATCTTCAGCTGACTGCCTATTATGTCGTGACCTGGATTGTGGGCGCCAAGCGTTACCATGA
  TTTGGACATTGATTATATTGGAATTTGGAATGAGAGGTCATATAATGCCAATTATATTAAGATATTAAGA
  AAAATGCTGAATTATCAAGGTCTCCAGCGAGTGAAAATCATAGCAAGTGATAATCTCTGGGAGTCCATCT
  CTGCATCCATGCTCCTTGATGCCGAACTCTTCAAGGTGGTTGATGTTATAGGGGCTCATTATCCTGGAAC
  CCATTCAGCAAAAGATGCAAAGTTGACTGGGAAGAAGCTTTGGTCTTCTGAAGACTTTAGCACTTTAAAT
  AGTGACATGGGTGCAGGCTGCTGGGGTCGCATTTTAAATCAGAATTATATCAATGGCTATATGACTTCCA
  CAATCGCATGGAATTTAGTGGCTAGTTACTATGAACAGTTGCCTTATGGGAGATGCGGGTTGATGACGGC
  CCAGGAGCCATGGAGTGGGCACTACGTGGTAGAATCTCCTGTCTGGGTATCAGCTCATACCACTCAGTTT
  ACTCAACCTGGCTGGTATTACCTGAAGACAGTTGGCCATTTAGAGAAAGGAGGAAGCTACGTAGCTCTGA
  CTGATGGCTTAGGGAACCTCACCATCATCATTGAAACCATGGTAAACTTTTGTTGTTGCTATTGGATTAA
  CTCACTTCTTTACTATTGGAAAAATAAAATATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001037525
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037525.2, NP_001032614.1
• RefSeq Size: 1380 bp
• RefSeq ORF: 1224 bp
• Locus ID: 2581
• Cytogenetics: 14q31.3
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: 'This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (b) has a distinct C-terminus and is shorter than isoform a.