TCN1 (NM_001062) Human Untagged Clone

CAT#: SC302965

TCN1 (untagged)-Human transcobalamin I (vitamin B12 binding protein, R binder family) (TCN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCN1
• Synonyms: HC; TC-1; TC1; TCI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001062 edited
  GGGGGTACACTGTTGGAGAGATGAGACAGTCACACCAGCTGCCCCTAGTGGGGCTCTTAC
  TGTTTTCTTTTATTCCAAGCCAACTATGCGAGATTTGTGAGGTAAGTGAAGAAAACTACA
  TCCGCCTAAAACCTCTGTTGAATACAATGATCCAGTCAAACTATAACAGGGGAACCAGCG
  CTGTCAATGTTGTGTTGTCCCTCAAACTTGTTGGAATCCAGATCCAAACCCTGATGCAAA
  AGATGATCCAACAAATCAAATACAATGTGAAAAGCAGATTGTCAGATGTAAGCTCGGGAG
  AGCTTGCCTTGATTATACTGGCTTTGGGAGTATGTCGTAACGCTGAGGAAAACTTAATAT
  ATGATTACCACCTGATCGACAAGCTAGAAAATAAATTCCAAGCAGAAATTGAAAATATGG
  AAGCACACAATGGCACTCCCCTGACTAACTACTACCAGCTCAGCCTGGACGTTTTGGCCT
  TGTGTCTGTTCAATGGGAACTACTCAACCGCCGAAGTTGTCAACCACTTCACTCCTGAAA
  ATAAAAACTATTATTTTGGTAGCCAGTTCTCAGTAGATACTGGTGCAATGGCTGTCCTGG
  CTCTGACCTGTGTGAAGAAGAGTCTAATAAATGGGCAGATCAAAGCAGATGAAGGCAGTT
  TAAAGAACATCAGTATTTATACAAAGTCACTGGTAGAAAAGATTCTGTCTGAGAAAAAAG
  AAAATGGTCTCATTGGAAACACATTTAGCACAGGAGAAGCCATGCAGGCCCTCTTTGTAT
  CATCAGACTATTATAATGAAAATGACTGGAATTGCCAACAAACTCTGAATACAGTGCTCA
  CGGAAATTTCTCAAGGAGCATTCAGTAATCCAAACGCTGCAGCCCAGGTCTTACCTGCCC
  TGATGGGAAAGACCTTCTTGGATATTAACAAAGACTCTTCTTGCGTCTCTGCTTCAGGTA
  ACTTCAACATCTCCGCTGATGAGCCTATAACTGTGACACCTCCTGACTCACAATCATATA
  TCTCCGTCAATTACTCTGTGAGAATCAATGAAACATATTTCACCAATGTCACTGTGCTAA
  ATGGTTCTGTCTTCCTCAGTGTGATGGAGAAAGCCCAGAAAATGAATGATACTATATTTG
  GTTTCACAATGGAGGAGCGCTCATGGGGGCCCTATATCACCTGTATTCAGGGCCTATGTG
  CCAACAATAATGACAGAACCTACTGGGAACTTCTGAGTGGAGGCGAACCACTGAGCCAAG
  GAGCTGGTAGTTACGTTGTCCGCAATGGATAAAACTTGGAGGTTCGCTGGAGCAAATACT
  AATAAGCCCAAACTTTCCTCAGCTGCATAAAGCCCATTTGCAGTGGAGTTCCATGTTTAT
  TGTCCTTATGCCTTCTTCTTCATTTATCCCAGTACGAGCAGGAGAGTTAATAACCTCCCC
  TTCTCTCTCTACATGTTCAATAAAAGTTGTTGAAAGATTAACAACTGTAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001062 unedited
  NNNGGTGCGGTTCACTTTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCCATTACG
  GCCGGGGGTACACTGTTGGAGAGATGAGACAGTCACACCAGCTGCCCCTAGTGGGGCTCT
  TACTGTTTTCTTTTATTCCAAGCCAACTATGCGAGATTTGTGAGGTAAGTGAAGAAAACT
  ACATCCGCCTAAAACCTCTGTTGAATACAATGATCCAGTCAAACTATAACAGGGGAACCA
  GCGCTGTCAATGTTGTGTTGTCCCTCAAACTTGTTGGAATCCAGATCCAAACCCTGATGC
  AAAAGATGATCCAACAAATCAAATACAATGTGAAAAGCAGATTGTCAGATGTAAGCTCGG
  GAGAGCTTGCCTTGATTATACTGGCTTTGGGAGTATGTCGTAACGCTGAGGAAAACTTAA
  TATATGATTACCACCTGATCGACAAGCTAGAAAATAAATTCCAAGCAGAAATTGAAAATA
  TGGAAGCACACAATGGCACTCCCCTGACTAACTACTACCAGCTCAGCCTGGACGTTTTGG
  CCTTGTGTCTGTTCAATGGGAACTACTCAACCGCCGAAGTTGTCAACCACTTCACTCCTG
  AAAATAAAAACTATTATTTTGGTAGCCAGTTCTCAGTAGATACTGGTGCAATGGCTGTCC
  TGGCTCTGACCTGTGTGAAGAAGAGTCTAATAAATGGGCAGATCAAAGCAGATGAAGGCA
  GTTTAAAGAACATCAGTATTTATACAAAGTCACTGGTAGAAAAGATTCTGTCTGAGAAAA
  AAGAAATGGTCTCATTGGAAACACATTTAGCACAGGAGAAGCCATGCAGGCCCTCTTTGT
  ATCATCAGACTATTATAATTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001062 unedited
  GGACACCATTTGTGATGGCACTTGCCAGGTCCAGNANAGCACTGGGGCAGGGTCACAGGG
  ATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGG
  GCCGAGGCGGCCGACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGTTGTTAAT
  CTTTCAACAACTTTTATTGAACATGTAGAGAGAGAAGGGGAGGTTATTAACTCTCCTGCT
  CGTACTGGGATAAATGAAGAAGAAGGCATAAGGACAATAAACATGGAACTCCACTGCAAA
  TGGACTTTATGCAGCTGAGGAAAGTTTGGGCTTATTAGTATTTGCTCCAGCGAACCTCCA
  AGTTTTCTCCATTGCGGACAACGTAACTACCAGCTCCTTGGCTCAGTGGTTCGCCTCCAC
  TCAGAAGTTCCCAGTAGGTTCTGTCATTATTGTTGGCACATAGGCCCTGAATACAGGTGA
  TATAGGGCCCCCATGAGCGCTCCTCCATTGTGAAACCAATATAGTATCATTCATTTTCTG
  GGCTTTCTCCATCACACTGAGGAAGACAGAACCATTTAGCACAGTGACATTGGTGAAATA
  TGTTTCATTGATTCTCACANATTATTTGACGGAAATTTATGATTGTGAGTCAAGAAGTGT
  CACAGTTTTTAGGCTCATCAACGGAAGATTTTTGAATTTACCTGAAACCCAAACCCCAAG
  AAAAATTCTTTTTTTAATTTCCAAGAAAGGTCTTTTCCCTTCAGGGCAAGTTAAAACCTT
  GGGCTTGAACCGTTTGGGATTTCTTGAATGCCTCTTTGAAAAATTTCCGGGAGCCCCTGG
  ATTTAAAAGTTTGTTGGGCATTTCCCAGCCATTTTCATTATAAAAAGCCGGAGAAACCAA
  AAGGGCCCGTATGGGTTTTTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001062
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001062.2, NP_001053.2
• RefSeq Size: 1543 bp
• RefSeq ORF: 1302 bp
• Locus ID: 6947
• Cytogenetics: 11q12.1
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This protein is a major constituent of secondary granules in neutrophils and facilitates the transport of cobalamin into cells. [provided by RefSeq, Jul 2008]'