Dystonin (DST) (NM_001723) Human Untagged Clone

CAT#: SC303058

DST (untagged)-Human dystonin (DST), transcript variant 1e

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DST
• Synonyms: BP240; BPA; BPAG1; CATX-15; CATX15; D6S1101; DMH; DT; EBSB2; HSAN6; MACF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001723, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCACAGTAGTAGTTATAGTTACCGTAGCAGTGATTCTGTGTTTAGTAACACTACCAGCACTCGAACCA
  GTCTTGATTCAAATGAAAATCTTCTCTTGGTTCATTGTGGTCCAACACTGATCAACTCTTGCATTAGCTT
  CGGCAGTGAATCCTTTGATGGACACAGGTTAGAAATGTTGCAACAGATTGCCAACAGAGTTCAGAGGGAC
  AGTGTCATCTGTGAAGACAAACTGATTCTTGCTGGAAATGCTCTTCAGTCTGATTCTAAAAGATTAGAAT
  CAGGAGTGCAGTTTCAGAATGAAGCAGAAATTGCTGGGTATATACTTGAATGTGAGAACCTTTTACGCCA
  GCATGTAATTGATGTACAGATTCTTATTGATGGAAAATACTACCAGGCAGATCAATTGGTACAGAGGGTT
  GCAAAACTGCGTGACGAAATTATGGCCTTAAGGAACGAATGTTCTTCTGTGTACAGCAAAGGACGCATAC
  TGACAACAGAACAGACAAAGCTCATGATATCAGGAATCACTCAAAGTTTAAACTCAGGATTTGCACAGAC
  CTTACACCCTAGTCTGACCTCAGGGCTGACCCAGAGTTTAACACCTTCCCTAACCTCTTCTAGTATGACT
  TCTGGCCTGTCATCAGGGATGACTTCCCGCCTGACTCCATCTGTCACTCCAGCTTATACACCTGGTTTCC
  CATCAGGATTAGTTCCAAATTTCAGTTCAGGAGTAGAGCCAAATTCATTGCAAACTTTGAAGTTGATGCA
  GATCCGAAAACCCCTTCTAAAGTCTTCTTTGCTGGATCAAAATTTAACAGAAGAAGAAATCAATATGAAA
  TTTGTTCAGGATCTTTTGAATTGGGTTGATGAGATGCAGGTACAACTGGACCGCACTGAGTGGGGCTCAG
  ATTTGCCAAGTGTTGAAAGCCATTTAGAAAATCATAAAAATGTTCATAGAGCTATTGAAGAATTTGAATC
  TAGTCTCAAAGAAGCTAAAATCAGTGAGATTCAAATGACAGCACCTCTTAAACTGACTTATGCAGAAAAG
  TTGCACAGATTAGAGAGTCAGTATGCAAAACTCTTGAATACATCCAGGAATCAAGAACGGCACCTTGATA
  CACTCCATAATTTTGTAAGTCGTGCGACTAATGAACTTATTTGGTTGAATGAAAAAGAAGAGGAGGAAGT
  TGCTTATGACTGGAGTGAGAGAAACACCAACATAGCTAGGAAAAAAGATTATCATGCTGAATTAATGAGA
  GAACTTGATCAAAAGGAAGAAAATATTAAATCAGTTCAGGAGATAGCAGAGCAGCTACTTCTAGAAAATC
  ATCCAGCCCGGTTAACTATTGAGGCCTACAGAGCGGCAATGCAGACGCAGTGGAGCTGGATCTTACAGCT
  CTGCCAGTGTGTGGAGCAGCACATAAAGGAGAACACAGCGTATTTCGAGTTTTTCAATGATGCCAAAGAA
  GCTACTGATTACTTAAGGAATCTAAAAGATGCCATTCAGCGGAAGTACAGCTGTGATAGATCAAGCAGCA
  TTCACAAGCTAGAAGACCTTGTTCAGGAATCAATGGAAGAGAAAGAAGAACTTCTGCAGTACAAAAGCAC
  TATAGCAAACCTAATGGGAAAAGCAAAAACAATAATTCAACTGAAGCCAAGGAATTCTGACTGTCCACTC
  AAAACTTCTATTCCGATCAAAGCTATCTGTGACTACAGACAAATTGAGATAACCATTTACAAAGACGATG
  AATGTGTTTTGGCGAATAACTCTCATCGTGCTAAATGGAAGGTCATTAGTCCTACTGGGAATGAGGCTAT
  GGTCCCATCTGTGTGCTTCACCGTTCCTCCACCAAACAAAGAAGCGGTGGACCTTGCCAACAGAATTGAG
  CAACAGTATCAGAATGTCCTGACTCTTTGGCATGAGTCTCACATAAACATGAAGAGTGTAGTATCCTGGC
  ATTATCTCATCAATGAAATTGATAGAATTCGAGCTAGCAATGTGGCTTCAATAAAGACAATGCTACCTGG
  TGAACATCAGCAAGTTCTAAGTAATCTACAATCTCGTTTTGAAGATTTTCTGGAAGATAGCCAGGAATCC
  CAAGTCTTTTCAGGCTCAGATATAACACAACTGGAAAAGGAGGTTAATGTATGTAAGCAGTATTATCAAG
  AACTTCTTAAATCTGCAGAAAGAGAGGAGCAAGAGGAATCAGTTTATAATCTCTACATCTCTGAAGTTCG
  AAACATTAGACTTCGGTTAGAGAACTGTGAAGATCGGCTGATTAGACAGATTCGAACTCCCCTGGAAAGA
  GATGATTTGCATGAAAGTGTGTTCAGAATCACAGAACAGGAGAAACTAAAGAAAGAGCTGGAACGACTTA
  AAGATGATTTGGGAACAATCACAAATAAGTGTGAGGAGTTTTTCAGTCAAGCAGCAGCCTCTTCATCAGT
  CCCTACCCTACGATCAGAGCTTAATGTGGTCCTTCAGAACATGAACCAAGTCTATTCTATGTCTTCCACT
  TACATAGATAAGTTGAAAACTGTTAACTTGGTGTTAAAAAACACTCAAGCTGCAGAAGCCCTCGTAAAAC
  TCTATGAAACTAAACTGTGTGAAGAAGAAGCAGTTATAGCTGACAAGAATAATATTGAGAATCTAATAAG
  TACTTTAAAGCAATGGAGATCTGAAGTAGATGAAAAGAGACAGGTATTCCATGCCTTAGAGGATGAGTTG
  CAGAAAGCTAAAGCCATCAGTGATGAAATGTTTAAAACGTATAAAGAACGGGACCTTGATTTTGACTGGC
  ACAAAGAAAAAGCAGATCAATTAGTTGAAAGGTGGCAAAATGTTCATGTGCAGATTGACAACAGGTTACG
  GGACTTAGAGGGCATTGGCAAATCACTGAAGTACTACAGAGACACTTACCATCCTTTAGATGATTGGATC
  CAGCAGGTTGAAACTACTCAGAGAAAGATTCAGGAAAATCAGCCTGAAAATAGTAAAACCCTAGCCACAC
  AGTTGAATCAACAGAAGATGCTGGTGTCCGAAATAGAAATGAAACAGAGCAAAATGGACGAGTGTCAAAA
  ATATGCAGAACAGTACTCAGCTACAGTGAAGGACTATGAATTACAAACAATGACCTACCGGGCCATGGTA
  GATTCACAACAAAAATCTCCAGTGAAACGCCGAAGAATGCAGAGTTCAGCAGATCTCATTATTCAAGAGT
  TCATGGACCTAAGGACTCGATATACTGCCCTGGTCACTCTCATGACACAATATATTAAATTTGCTGGTGA
  TTCATTGAAGAGGCTGGAAGAGGAGGAGATTAAAAGGTGTAAGGAGACTTCTGAACATGGGGCATATTCA
  GATCTGCTTCAGCGTCAGAAGGCAACAGTGCTTGAGAATAGCAAACTTACAGGAAAGATAAGTGAGTTGG
  AAAGAATGGTAGCTGAACTAAAGAAACAAAAGTCCCGAGTAGAGGAAGAACTTCCGAAGGTCAGGGAGGC
  TGCAGAAAATGAATTGAGAAAGCAGCAGAGAAATGTAGAAGATATCTCTCTGCAGAAGATAAGGGCTGAA
  AGTGAAGCCAAGCAGTACCGCAGGGAACTTGAAACCATTGTGAGAGAGAAGGAAGCCGCTGAAAGAGAAC
  TGGAGCGGGTGAGGCAGCTCACCATAGAGGCCGAGGCTAAAAGAGCTGCCGTGGAAGAGAACCTCCTGAA
  TTTTCGCAATCAGTTGGAGGAAAACACCTTTACCAGACGAACACTGGAAGATCATCTTAAAAGAAAAGAT
  TTAAGTCTCAATGATTTGGAGCAACAAAAAAATAAATTAATGGAAGAATTAAGAAGAAAGAGAGACAATG
  AGGAAGAACTCTTGAAGCTGATAAAGCAGATGGAAAAAGACCTTGCATTTCAGAAACAGGTAGCAGAGAA
  ACAGTTGAAAGAAAAGCAGAAAATTGAATTGGAAGCAAGAAGAAAAATAACTGAAATTCAGTATACATGT
  AGAGAAAATGCATTGCCAGTGTGTCCGATCACACAGGCTACATCATGCAGGGCAGTAACGGGTCTCCAGC
  AAGAACATGACAAGCAGAAAGCAGAAGAACTCAAACAGCAGGTAGATGAACTAACAGCTGCCAATAGAAA
  GGCTGAACAAGACATGAGAGAGCTGACATATGAACTTAATGCCCTCCAGCTTGAAAAAACGTCATCTGAG
  GAAAAGGCTCGTTTGCTAAAAGATAAACTAGATGAAACAAATAATACACTCAGATGCCTTAAGTTGGAGC
  TGGAAAGGAAGGATCAGGCGGAGAAAGGGTATTCTCAACAACTCAGAGAGCTTGGTAGGCAATTGAATCA
  AACCACAGGTAAAGCTGAAGAAGCCATGCAAGAAGCTAGTGATCTCAAGAAAATAAAGCGCAATTATCAG
  TTAGAATTAGAATCTCTTAATCATGAAAAAGGGAAACTACAAAGAGAAGTAGACAGAATCACAAGGGCAC
  ATGCTGTAGCTGAGAAGAATATTCAGCATTTAAATTCACAAATTCATTCTTTTCGAGATGAGAAAGAATT
  AGAAAGACTACAAATCTGCCAGAGAAAATCAGATCATCTAAAAGAACAATTTGAGAAAAGCCATGAGCAG
  TTGCTTCAAAATATCAAAGCTGAAAAAGAAAATAATGATAAAATCCAAAGGCTCAATGAAGAATTGGAGA
  AAAGTAATGAGTGTGCAGAGATGCTAAAACAAAAAGTAGAGGAGCTTACTAGGCAGAATAATGAAACCAA
  ATTAATGATGCAGAGAATTCAGGCAGAATCAGAGAATATAGTTTTAGAGAAACAAACTATCCAGCAAAGA
  TGTGAAGCACTGAAAATTCAGGCAGATGGTTTTAAAGATCAGCTACGCAGCACAAATGAACACTTGCATA
  AACAGACAAAAACAGAGCAGGATTTTCAAAGAAAAATTAAATGCCTAGAAGAAGACCTGGCGAAAAGTCA
  AAATTTGGTAAGTGAATTTAAGCAAAAGTGTGACCAACAGAACATTATCATCCAGAATACCAAGAAAGAA
  GTTAGAAATCTGAATGCGGAACTGAATGCTTCCAAAGAAGAGAAGCGACGCGGGGAGCAGAAAGTTCAGC
  TACAACAAGCTCAGGTGCAAGAGTTAAATAACAGGTTGAAAAAAGTACAAGACGAATTACACTTAAAGAC
  CATAGAGGAGCAGATGACCCACAGAAAGATGGTTCTGTTTCAGGAAGAATCTGGTAAATTCAAACAATCA
  GCAGAGGAGTTTCGGAAGAAGATGGAAAAATTAATGGAGTCCAAAGTCATCACTGAAAATGATATTTCAG
  GCATTAGGCTTGACTTTGTGTCTCTTCAACAAGAAAACTCTAGAGCCCAAGAAAATGCTAAGCTTTGTGA
  AACAAACATTAAAGAACTTGAAAGACAGCTTCAACAGTATCGTGAACAAATGCAGCAAGGGCAGCACATG
  GAAGCAAATCATTACCAAAAATGTCAGAAACTTGAGGATGAGCTGATAGCCCAGAAGCGTGAGGTTGAAA
  ACCTGAAGCAAAAAATGGACCAACAGATCAAAGAGCATGAACATCAATTAGTTTTGCTCCAGTGTGAAAT
  TCAAAAAAAGAGCACAGCCAAAGACTGTACCTTCAAACCAGATTTTGAGATGACAGTGAAGGAGTGCCAG
  CACTCTGGAGAGCTGTCCTCTAGAAACACTGGACACCTTCACCCAACACCCAGATCCCCTCTGTTGAGAT
  GGACTCAAGAACCACAGCCATTGGAAGAGAAGTGGCAGCATCGGGTTGTTGAACAGATACCCAAAGAAGT
  CCAATTCCAGCCACCAGGGGCTCCACTCGAGAAAGAGAAAAGCCAGCAGTGTTACTCTGAGTACTTTTCT
  CAGACAAGCACCGAGTTACAGATAACTTTTGATGAGACAAACCCCATTACAAGACTGTCTGAAATTGAGA
  AGATAAGAGACCAAGCCCTGAACAATTCTAGACCACCTGTTAGGTATCAAGATAACGCATGTGAAATGGA
  ACTGGTGAAGGTTTTGACACCCTTAGAGATAGCTAAGAACAAGCAGTATGATATGCATACAGAAGTCACA
  ACATTAAAACAAGAAAAGAACCCAGTTCCCAGTGCTGAAGAATGGATGCTTGAAGGGTGCAGAGCATCTG
  GTGGACTCAAGAAAGGGGATTTCCTTAAGAAGGGCTTAGAACCAGAGACCTTCCAGAACTTTGATGGTGA
  TCATGCATGTTCAGTCAGGGATGATGAATTTAAATTCCAAGGGCTTAGGCACACTGTGACTGCCAGGCAG
  TTGGTGGAAGCTAAGCTTCTGGACATGAGAACAATTGAGCAGCTGCGACTCGGTCTTAAGACTGTTGAAG
  AAGTTCAGAAAACTCTTAACAAGTTTCTGACGAAAGCCACCTCAATTGCAGGGCTTTACCTAGAATCTAC
  AAAAGAAAAGATTTCATTTGCCTCAGCGGCCGAGAGAATCATAATAGACAAAATGGTGGCTTTGGCATTT
  TTAGAAGCTCAGGCTGCAACAGGTTTTATAATTGATCCCATTTCAGGTCAGACATATTCTGTTGAAGATG
  CAGTTCTTAAAGGAGTTGTTGACCCCGAATTCAGAATTAGGCTTCTTGAGGCAGAGAAGGCAGCTGTGGG
  ATATTCTTATTCTTCTAAGACATTGTCAGTGTTTCAAGCTATGGAAAATAGAATGCTTGACAGACAAAAA
  GGTAAACATATCTTGGAAGCCCAGATTGCCAGTGGGGGTGTCATTGACCCTGTGAGAGGCATTCGTGTTC
  CTCCAGAAATTGCTCTGCAGCAGGGGTTGTTGAATAATGCCATCTTACAGTTTTTACATGAGCCATCCAG
  CAACACAAGAGTTTTCCCTAATCCCAATAACAAGCAAGCTCTGTATTACTCAGAATTACTGCGAATGTGT
  GTATTTGATGTAGAGTCCCAATGCTTTCTGTTTCCATTTGGGGAGAGGAACATTTCCAATCTCAATGTCA
  AGAAAACACATAGAATTTCTGTAGTAGATACTAAAACAGGATCAGAATTGACCGTGTATGAGGCTTTCCA
  GAGAAACCTGATTGAGAAAAGTATATATCTTGAACTTTCAGGGCAGCAATATCAGTGGAAGGAAGCTATG
  TTTTTTGAATCCTATGGGCATTCTTCTCATATGCTGACTGATACTAAAACAGGATTACACTTCAATATTA
  ATGAGGCTATAGAGCAGGGAACAATTGACAAAGCCTTGGTCAAAAAGTATCAGGAAGGCCTCATCACACT
  TACAGAACTTGCTGATTCTTTGCTGAGCCGGTTAGTCCCCAAGAAAGATTTGCACAGTCCTGTTGCAGGG
  TATTGGCTGACTGCTAGTGGGGAAAGGATCTCTGTACTAAAAGCCTCCCGTAGAAATTTGGTTGATCGGA
  TTACTGCCCTCCGATGCCTTGAAGCCCAAGTCAGTACAGGGGGCATAATTGATCCTCTTACTGGCAAAAA
  GTACCGGGTGGCCGAAGCTTTGCATAGAGGCCTGGTTGATGAGGGGTTTGCCCAGCAGCTGCGACAGTGT
  GAATTAGTAATCACAGGGATTGGCCATCCCATCACTAACAAAATGATGTCAGTGGTGGAAGCTGTGAATG
  CAAATATTATAAATAAGGAAATGGGAATCCGATGTTTGGAATTTCAGTACTTGACAGGAGGGTTGATAGA
  GCCACAGGTTCACTCTCGGTTATCAATAGAAGAGGCTCTCCAAGTAGGTATTATAGATGTCCTCATTGCC
  ACAAAACTCAAAGATCAAAAGTCATATGTCAGAAATATAATATGCCCTCAGACAAAAAGAAAGTTGACAT
  ATAAAGAAGCCTTAGAAAAAGCTGATTTTGATTTCCACACAGGACTTAAACTGTTAGAAGTATCTGAGCC
  CCTGATGACAGGAATTTCTAGCCTCTACTATTCTTCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001723
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001723.5, NP_001714.1
• RefSeq Size: 9006 bp
• RefSeq ORF: 7950 bp
• Locus ID: 667
• Cytogenetics: 6p12.1
• Gene Summary: 'This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1e) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 2. This variant also differs in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1e) has shorter and distinct N- and C-termini compared to isoform 2.