Calcitonin (CALCA) (NM_001741) Human Untagged Clone

CAT#: SC303063

CALCA (untagged)-Human calcitonin-related polypeptide alpha (CALCA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALCA
• Synonyms: CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001741 edited
  GGGCCGGCCGCGAATTCATGGGCTTCCAAAAGTTCTCCCCCTTCCTGGCTCTCAGCATCT
  TGGTCCTGTTGCAGGCAGGCAGCCTCCATGCAGCACCATTCAGGTCTGCCCTGGAGAGCA
  GCCCAGCAGACCCGGCCACGCTCAGTGAGGACGAAGCGCGCCTCCTGCTGGCTGCACTGG
  TGCAGGACTATGTGCAGATGAAGGCCAGTGAGCTGGAGCAGGAGCAAGAGAGAGAGGGCT
  CCAGCCTGGACAGCCCCAGATCTAAGCGGTGCGGTAATCTGAGTACTTGCATGCTGGGCA
  CATACACGCAGGACTTCAACAAGTTTCACACGTTCCCCCAAACTGCAATTGGGGTTGGAG
  CACCTGGAAAGAAAAGGGATATGTCCAGCGACTTGGAGAGAGACCATCGCCCTCATGTTA
  GCATGCCCCAGAATGCCAACTAACTCGACTCTAGATTGCGGCCGCGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001741 unedited
  GACAAAATTTGTAATACGACTCACTATAGGGNNCGGCCGCGNNAATTCATGGGCTTCCAA
  AAGTTCTCCCCCTTCCTGGCTCTCAGCATCTTGGTCCTGTTGCAGGCAGGCAGCCTCCAT
  GCAGCACCATTCAGGTCTGCCCTGGAGAGCAGCCCAGCAGACCCGGCCACGCTCAGTGAG
  GACGAAGCGCGCCTCCTGCTGGCTGCACTGGTGCAGGACTATGTGCAGATGAAGGCCAGT
  GAGCTGGAGCAGGAGCAAGAGAGAGAGGGCTCCAGCCTGGACAGCCCCAGATCTAAGCGG
  TGCGGTAATCTGAGTACTTGCATGCTGGGCACATACACGCAGGACTTCAACAAGTTTCAC
  ACGTTCCCCCAAACTGCAATTGGGGTTGGAGCACCTGGAAAGAAAAGGGATATGTCCAGC
  GACTTGGAGAGAGACCATCGCCCTCATGTTAGCATGCCCCAGAATGCCAACTAACTCGAC
  TCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTG
  ACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG
  TCCTAATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGG
  GTGGAGGGGGGGTGGTATGGGAGCAAGGGGCNAAGTGGGAAGNACACCTGTNAGGCCTGC
  GGGGTCTATTGGGGACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCNAATCTC
  CGCTCCCTGGTTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGGATTCCAGC
  ATGCATGACCAGGCTCAGCTAATTTTTGGTTTTTTTGGTAGAGACGGGGTTTCACCATAT
  TGGCCAAGCTGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_001741
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001741.2, NP_001732.1
• RefSeq Size: 792 bp
• RefSeq ORF: 426 bp
• Locus ID: 796
• Cytogenetics: 11p15.2
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]'
  Transcript Variant: This variant (1) differs in the 5' and 3' UTRs and the coding region compared to variant 3. The resulting protein has a longer and distinct C-terminus compared to calcitonin gene-related peptide 1. Variants 1 and 2 encode the same protein, which is cleaved to yield calcitonin (CT), a thyroid peptide-hormone product, and katacalcin peptides.