Collagen IV alpha 6 (COL4A6) (NM_001847) Human Untagged Clone

CAT#: SC303081

COL4A6 (untagged)-Human collagen, type IV, alpha 6 (COL4A6), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL4A6
• Synonyms: CXDELq22.3; DELXq22.3; DFNX6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001847, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTATAAACAAGTTGTGGCTGCTCCTGGTTACGTTGTGCCTGACCGAGGAACTGGCAGCAGCGGGAG
  AGAAGTCTTATGGAAAGCCATGTGGGGGCCAGGACTGCAGTGGGAGCTGTCAGTGTTTTCCTGAGAAAGG
  AGCGAGAGGACGACCTGGACCAATTGGAATTCAAGGCCCAACAGGTCCTCAAGGATTCACTGGCTCTACT
  GGTTTATCGGGATTGAAAGGAGAAAGGGGTTTCCCAGGCCTTCTGGGACCTTATGGACCAAAAGGAGATA
  AGGGTCCCATGGGAGTTCCTGGCTTTCTTGGCATCAATGGGATTCCGGGCCACCCTGGACAACCAGGCCC
  CAGAGGCCCACCTGGTCTGGATGGCTGTAATGGAACTCAAGGAGCTGTTGGATTTCCAGGCCCTGATGGC
  TATCCTGGGCTTCTCGGACCACCCGGGCTTCCTGGTCAGAAAGGATCAAAAGGTGACCCTGTCCTTGCTC
  CAGGTAGTTTCAAAGGAATGAAGGGGGATCCTGGGCTGCCTGGACTGGATGGAATCACTGGCCCACAAGG
  AGCACCCGGATTTCCTGGAGCTGTAGGACCTGCAGGACCACCAGGATTACAAGGTCCTCCAGGGCCTCCT
  GGTCCTCTTGGTCCTGATGGGAATATGGGGCTAGGTTTTCAAGGAGAGAAAGGAGTCAAGGGGGATGTTG
  GCCTCCCTGGCCCAGCAGGACCTCCACCATCTACTGGAGAGCTGGAATTCATGGGATTCCCCAAAGGGAA
  GAAAGGATCCAAGGGTGAACCAGGGCCTAAGGGTTTTCCAGGCATAAGTGGCCCTCCAGGCTTCCCGGGC
  CTTGGAACTACTGGAGAAAAGGGAGAAAAGGGAGAAAAGGGAATCCCTGGTTTGCCAGGACCTAGGGGTC
  CCATGGGTTCAGAAGGAGTCCAAGGCCCTCCAGGGCAACAGGGCAAGAAAGGGACCCTGGGATTTCCTGG
  GCTTAATGGATTCCAAGGAATTGAGGGTCAAAAGGGTGACATTGGCCTGCCAGGCCCAGATGTTTTCATC
  GATATAGATGGTGCTGTGATCTCAGGTAATCCTGGAGATCCTGGTGTACCTGGCCTCCCAGGCCTTAAAG
  GAGATGAAGGCATCCAAGGCCTACGTGGCCCTTCTGGTGTCCCTGGATTGCCAGCATTATCAGGTGTCCC
  AGGAGCCCTAGGGCCTCAGGGATTTCCAGGGCTGAAGGGGGACCAAGGAAACCCAGGCCGTACCACAATT
  GGAGCAGCTGGCCTCCCTGGCAGAGATGGTTTGCCAGGCCCACCAGGTCCACCAGGCCCACCTAGTCCAG
  AATTTGAGACTGAAACTCTACACAACAAAGAGTCAGGGTTCCCTGGTCTCCGAGGAGAACAAGGTCCAAA
  AGGAAACCTAGGCCTCAAAGGAATAAAAGGAGACTCAGGTTTCTGTGCTTGTGACGGTGGTGTTCCCAAC
  ACTGGACCACCCGGGGAACCAGGCCCACCTGGTCCATGGGGTCTCATAGGCCTTCCAGGCCTTAAAGGAG
  CCAGAGGAGATCGAGGCTCTGGGGGTGCACAGGGCCCAGCAGGGGCTCCAGGCTTAGTTGGGCCTCTGGG
  TCCTTCAGGACCCAAAGGAAAGAAGGGGGAACCAATTCTCAGTACAATCCAAGGAATGCCAGGAGATCGG
  GGTGATTCTGGCTCCCAGGGCTTCCGTGGTGTAATAGGAGAACCAGGCAAGGACGGAGTACCAGGTTTAC
  CAGGTCTGCCAGGCCTTCCGGGTGATGGTGGACAGGGCTTCCCAGGTGAAAAGGGGTTACCTGGACTTCC
  TGGTGAAAAAGGCCATCCTGGTCCACCTGGCCTCCCAGGAAATGGGTTACCAGGACTTCCTGGACCCCGT
  GGGCTTCCTGGAGATAAAGGCAAGGATGGATTACCGGGACAACAAGGCCTTCCCGGATCTAAGGGAATCA
  CCCTGCCCTGTATTATTCCTGGGTCATACGGTCCATCAGGATTTCCAGGCACTCCCGGATTCCCAGGCCC
  TAAAGGGTCTCGAGGCCTCCCTGGGACCCCAGGCCAGCCTGGGTCAAGTGGAAGTAAAGGAGAGCCAGGG
  AGTCCAGGATTGGTTCATCTTCCTGAATTACCAGGATTTCCTGGACCTCGTGGGGAGAAGGGCTTGCCTG
  GGTTTCCTGGGCTCCCTGGAAAAGATGGCTTGCCTGGGATGATTGGCAGTCCAGGCTTACCTGGTTCCAA
  GGGAGCCACTGGTGACATCTTTGGTGCTGAAAATGGTGCTCCGGGGGAACAAGGCCTACAAGGATTAACA
  GGGCACAAAGGATTTCTTGGAGACTCTGGCCTTCCAGGACTCAAGGGTGTGCACGGGAAGCCTGGCTTAC
  TAGGCCCCAAAGGTGAGCGGGGCAGCCCTGGGACACCAGGACAGGTGGGACAGCCAGGCACCCCAGGATC
  TAGTGGTCCATATGGCATCAAGGGCAAATCTGGGCTCCCAGGAGCACCAGGCTTCCCAGGCATCTCAGGA
  CATCCTGGAAAGAAAGGAACAAGAGGCAAGAAAGGTCCTCCTGGATCAATTGTAAAGAAAGGGCTGCCAG
  GGCTAAAAGGCCTTCCTGGAAATCCAGGCCTAGTAGGACTGAAAGGAAGCCCAGGCTCTCCAGGGGTCGC
  TGGGTTGCCAGCCCTCTCTGGACCCAAGGGAGAGAAGGGGTCTGTTGGATTCGTAGGTTTTCCAGGAATA
  CCAGGTCTGCCTGGTATTCCTGGAACAAGAGGATTAAAAGGAATTCCAGGATCAACTGGAAAAATGGGAC
  CATCTGGACGTGCTGGTACTCCTGGTGAAAAGGGAGACAGAGGCAATCCGGGGCCAGTCGGAATACCTAG
  TCCAAGACGTCCAATGTCAAACCTTTGGCTCAAAGGAGACAAAGGCTCTCAAGGCTCAGCCGGATCCAAT
  GGATTTCCTGGGCCAAGAGGTGACAAAGGAGAGGCTGGTCGACCTGGACCACCAGGCCTACCTGGAGCTC
  CTGGCCTCCCAGGCATTATCAAAGGAGTTAGTGGAAAGCCAGGGCCCCCTGGCTTCATGGGAATCCGGGG
  CTTACCTGGCCTGAAGGGGTCCTCTGGGATCACAGGTTTCCCAGGAATGCCAGGAGAAAGTGGTTCACAA
  GGTATCAGAGGGTCGCCTGGACTCCCAGGAGCATCTGGTCTCCCAGGCCTGAAAGGAGACAACGGCCAGA
  CAGTTGAAATTTCCGGTAGCCCAGGACCCAAGGGACAGCCTGGCGAATCTGGTTTTAAAGGCACAAAAGG
  AAGAGATGGACTAATAGGCAATATAGGCTTCCCTGGAAACAAAGGTGAAGATGGAAAAGTTGGTGTTTCT
  GGAGATGTTGGCCTTCCTGGAGCTCCAGGATTTCCAGGAGTTGCCGGCATGAGAGGAGAACCAGGACTTC
  CAGGTTCTTCTGGTCACCAAGGGGCAATTGGGCCTCTAGGATCCCCCGGATTAATAGGACCCAAAGGCTT
  CCCTGGATTTCCTGGTTTACATGGACTGAATGGGCTTCCGGGCACCAAGGGTACCCATGGCACTCCAGGA
  CCTAGTATCACCGGTGTGCCTGGGCCTGCTGGTCTCCCTGGACCCAAAGGAGAAAAAGGATATCCAGGAA
  TTGGCATCGGAGCTCCAGGGAAGCCGGGCCTGAGAGGGCAAAAAGGTGATCGAGGTTTCCCAGGTCTCCA
  GGGCCCTGCTGGTCTCCCCGGTGCCCCAGGCATCTCCTTGCCCTCACTCATAGCAGGACAGCCTGGTGAC
  CCCGGGCGACCAGGCCTAGATGGAGAACGAGGCCGCCCAGGCCCCGCTGGACCCCCAGGTCCCCCTGGGC
  CATCCTCGAATCAAGGCGACACCGGAGACCCTGGCTTCCCTGGAATTCCTGGACCTAAAGGGCCTAAGGG
  AGACCAAGGAATTCCAGGTTTTTCTGGCCTCCCTGGAGAGCTAGGACTGAAAGGCATGAGAGGTGAGCCT
  GGCTTCATGGGGACTCCAGGCAAGGTTGGGCCACCTGGAGACCCAGGATTTCCCGGAATGAAGGGGAAGG
  CAGGGCCAAGAGGCTCTTCTGGCCTCCAAGGTGATCCTGGACAAACACCAACTGCAGAAGCTGTCCAGGT
  TCCTCCTGGACCCTTGGGTCTACCAGGGATCGATGGCATCCCTGGCCTCACTGGGGACCCTGGGGCTCAA
  GGCCCTGTAGGCCTACAAGGCTCCAAAGGTTTACCTGGCATCCCCGGTAAAGATGGCCCCAGTGGGCTCC
  CAGGCCCACCTGGGGCTCTTGGTGATCCTGGTCTGCCTGGACTGCAAGGCCCTCCAGGATTTGAAGGAGC
  TCCAGGGCAGCAAGGCCCCTTCGGGATGCCTGGAATGCCTGGCCAGAGCATGAGAGTGGGCTACACGTTG
  GTAAAGCACAGCCAGTCGGAACAGGTGCCCCCGTGTCCCATCGGGATGAGCCAGCTGTGGGTGGGGTACA
  GCTTACTGTTTGTGGAGGGGCAAGAGAAAGCCCACAACCAGGACCTGGGCTTTGCTGGCTCCTGTCTGCC
  CCGCTTCAGCACCATGCCCTTCATCTACTGCAACATCAACGAGGTGTGCCACTATGCCAGGCGCAATGAT
  AAATCTTACTGGCTCTCCACTACCGCCCCTATCCCCATGATGCCCGTCAGCCAGACCCAGATTCCCCAGT
  ACATCAGCCGCTGCTCTGTGTGTGAGGCACCCTCGCAAGCCATTGCTGTGCACAGCCAGGACATCACCAT
  CCCGCAGTGCCCCCTGGGCTGGCGCAGCCTCTGGATTGGGTACTCTTTCCTCATGCACACTGCCGCTGGT
  GCCGAGGGTGGAGGCCAGTCCCTGGTCTCACCTGGCTCCTGCCTAGAGGACTTTCGGGCCACTCCTTTCA
  TCGAATGCAGTGGTGCCCGAGGCACCTGCCACTACTTTGCAAACAAGTACAGTTTCTGGTTGACCACAGT
  GGAGGAGAGGCAGCAGTTTGGGGAGTTGCCTGTGTCTGAAACGCTGAAAGCTGGGCAGCTCCACACTCGA
  GTCAGTCGCTGCCAGGTGTGTATGAAAAGCCTGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001847
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001847.3, NP_001838.2
• RefSeq Size: 6618 bp
• RefSeq ORF: 5076 bp
• Locus ID: 1288
• Cytogenetics: Xq22.3
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (A) utilizes a different alternative exon in the 5' UTR and coding region, exon 1A, which results in use of an alternate upstream translation start, compared to variant 3. The resulting protein (isoform A) has a distinct and shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.