Histidase (HAL) (NM_002108) Human Untagged Clone

CAT#: SC303122

HAL (untagged)-Human histidine ammonia-lyase (HAL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HAL
• Synonyms: HIS; HSTD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002108, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCAGATACACGGTGCACGTACGTGGGGAATGGCTGGCAGTGCCCTGCCAGGACGCGCAGCTCACTG
  TGGGCTGGCTGGGCCGGGAGGCCGTGAGGCGCTATATCAAGAATAAGCCCGACAATGGTGGCTTCACCTC
  CGTGGATGACGCGCACTTCCTTGTGCGCCGGTGCAAGGGCCTGGGCCTGCTGGACAACGAGGACCGGCTC
  GAGGTGGCCCTAGAGAACAACGAGTTCGTGGAAGTGGTTATAGAGGGTGATGCCATGTCTCCTGACTTCA
  TTCCATCTCAACCAGAAGGAGTTTATCTATACAGCAAGTACCGGGAGCCTGAAAAGTACATCGAGTTAGA
  TGGAGACCGTCTGACCACGGAGGATCTGGTCAACTTGGGAAAGGGACGCTACAAAATAAAGCTCACCCCA
  ACAGCTGAGAAGAGGGTGCAGAAATCCAGGGAGGTCATAGATAGCATCATAAAAGAGAAAACAGTTGTTT
  ACGGTATTACTACAGGTTTTGGGAAATTTGCCAGAACTGTAATTCCTATCAATAAGCTACAGGAGCTTCA
  GGTCAACTTAGTACGCTCACATTCTTCAGGTGTTGGGAAACCACTAAGTCCTGAGAGGTGTCGGATGCTC
  TTGGCTTTAAGGATCAATGTCTTAGCCAAAGGATACAGTGGCATTTCCCTGGAGACCCTCAAACAAGTCA
  TAGAAATGTTTAATGCCTCCTGCCTGCCCTATGTCCCAGAGAAAGGAACCGTTGGTGCCAGTGGAGACCT
  TGCCCCACTCTCTCATCTTGCTCTTGGGCTAGTTGGAGAAGGGAAGATGTGGTCTCCGAAGAGTGGCTGG
  GCTGATGCTAAATACGTGCTAGAAGCCCATGGATTGAAACCAGTTATTTTAAAACCAAAAGAGGGCCTGG
  CACTCATCAATGGGACGCAGATGATCACATCCCTGGGCTGTGAAGCTGTAGAGCGAGCCAGTGCTATTGC
  ACGGCAGGCTGACATTGTGGCAGCCCTGACCCTTGAGGTGCTGAAGGGCACCACCAAAGCCTTTGACACT
  GACATTCATGCTCTTCGACCTCACCGTGGGCAAATTGAAGTTGCTTTTCGGTTTCGGTCACTCTTGGACT
  CAGATCACCACCCATCAGAAATAGCAGAGAGTCACAGGTTCTGTGATCGCGTCCAGGATGCATACACCTT
  GCGCTGCTGTCCACAGGTCCATGGTGTGGTGAATGATACAATAGCATTTGTGAAGAACATCATTACCACA
  GAACTGAACAGCGCAACAGATAATCCTATGGTCTTTGCCAATAGGGGAGAGACAGTTTCTGGAGGAAACT
  TCCATGGTGAATACCCAGCCAAAGCCCTAGACTACTTGGCCATTGGCATCCATGAACTTGCTGCAATCAG
  TGAGAGAAGAATCGAGCGGCTCTGCAATCCCTCCCTCAGTGAGCTGCCTGCCTTCCTGGTGGCTGAAGGT
  GGTCTGAACTCTGGGTTCATGATAGCTCACTGCACGGCAGCAGCCCTTGTTTCTGAGAACAAGGCTCTGT
  GCCATCCCTCGTCTGTTGACTCCCTCTCCACCAGCGCAGCCACGGAGGACCACGTCTCCATGGGAGGATG
  GGCAGCAAGGAAAGCCCTCAGGGTCATCGAGCATGTGGAGCAAGTGCTGGCCATCGAGCTCCTTGCAGCC
  TGCCAGGGCATAGAGTTTCTACGTCCCCTGAAAACAACCACTCCGCTGGAGAAGGTCTATGACCTGGTGC
  GCTCTGTTGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCGGACATCGAGGCAGCCCACAGGCTGCT
  CCTGGAGCAGAAGGTTTGGGAAGTAGCTGCTCCATACATTGAAAAATACAGAATGGAGCATATTCCAGAA
  TCAAGACCTCTTTCTCCAACAGCCTTTTCACTGCAATTTCTGCACAAGAAATCCACCAAAATCCCGGAGT
  CTGAGGACCTTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002108
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002108.3, NP_002099.1
• RefSeq Size: 3927 bp
• RefSeq ORF: 1974 bp
• Locus ID: 3034
• Cytogenetics: 12q23.1
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: 'Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.