LCT (NM_002299) Human Untagged Clone

CAT#: SC303171

LCT (untagged)-Human lactase (LCT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCT
• Synonyms: LAC; LPH; LPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002299, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCTGTCTTGGCATGTAGTCTTTATTGCCCTGCTAAGTTTTTCATGCTGGGGGTCAGACTGGGAGT
  CTGATAGAAATTTCATTTCCACCGCTGGTCCTCTAACCAATGACTTGCTGCACAACCTGAGTGGTCTCCT
  GGGAGACCAGAGTTCTAACTTTGTAGCAGGGGACAAAGACATGTATGTTTGTCACCAGCCACTGCCCACT
  TTCCTGCCAGAATACTTCAGCAGTCTCCATGCCAGTCAGATCACCCATTATAAGGTATTTCTGTCATGGG
  CACAGCTCCTCCCAGCAGGAAGCACCCAGAATCCAGACGAGAAAACAGTGCAGTGCTACCGGCGACTCCT
  CAAGGCCCTCAAGACTGCACGGCTTCAGCCCATGGTCATCCTGCACCACCAGACCCTCCCTGCCAGCACC
  CTCCGGAGAACCGAAGCCTTTGCTGACCTCTTCGCCGACTATGCCACATTCGCCTTCCACTCCTTCGGGG
  ACCTAGTTGGGATCTGGTTCACCTTCAGTGACTTGGAGGAAGTGATCAAGGAGCTTCCCCACCAGGAATC
  AAGAGCGTCACAACTCCAGACCCTCAGTGATGCCCACAGAAAAGCCTATGAGATTTACCACGAAAGCTAT
  GCTTTTCAGGGCGGAAAACTCTCTGTTGTCCTGCGAGCTGAAGATATCCCGGAGCTCCTGCTAGAACCAC
  CCATATCTGCGCTTGCCCAGGACACGGTCGATTTCCTCTCTCTTGATTTGTCTTATGAATGCCAAAATGA
  GGCAAGTCTGCGGCAGAAGCTGAGTAAATTGCAGACCATTGAGCCAAAAGTGAAAGTTTTCATCTTCAAC
  CTAAAACTCCCAGACTGCCCCTCCACCATGAAGAACCCAGCCAGTCTGCTCTTCAGCCTTTTTGAAGCCA
  TAAATAAAGACCAAGTGCTCACCATTGGGTTTGATATTAATGAGTTTCTGAGTTGTTCATCAAGTTCCAA
  GAAAAGCATGTCTTGTTCTCTGACTGGCAGCCTGGCCCTTCAGCCTGACCAGCAGCAGGACCACGAGACC
  ACGGACTCCTCTCCTGCCTCTGCCTATCAGAGAATCTGGGAAGCATTTGCCAATCAGTCCAGGGCGGAAA
  GGGATGCCTTCCTGCAGGATACTTTCCCTGAAGGCTTCCTCTGGGGTGCCTCCACAGGAGCCTTTAACGT
  GGAAGGAGGCTGGGCCGAGGGTGGGAGAGGGGTGAGCATCTGGGATCCACGCAGGCCCCTGAACACCACT
  GAGGGCCAAGCGACGCTGGAGGTGGCCAGCGACAGTTACCACAAGGTAGCCTCTGACGTCGCCCTGCTTT
  GCGGCCTCCGGGCTCAGGTGTACAAGTTCTCCATCTCCTGGTCCCGGATCTTCCCCATGGGGCACGGGAG
  CAGCCCCAGCCTCCCAGGCGTTGCCTACTACAACAAGCTGATTGACAGGCTACAGGATGCGGGCATCGAG
  CCCATGGCCACGCTGTTCCACTGGGACCTGCCTCAGGCCCTGCAGGATCATGGTGGATGGCAGAATGAGA
  GCGTGGTGGATGCCTTCCTGGACTATGCGGCCTTCTGCTTCTCCACATTTGGGGACCGTGTGAAGCTGTG
  GGTGACCTTCCATGAGCCGTGGGTGATGAGCTACGCAGGCTATGGCACCGGCCAGCACCCTCCCGGCATC
  TCTGACCCAGGAGTGGCCTCTTTTAAGGTGGCTCACTTGGTCCTCAAGGCTCATGCCAGAACTTGGCACC
  ACTACAACAGCCATCATCGCCCACAGCAGCAGGGGCACGTGGGCATTGTGCTGAACTCAGACTGGGCAGA
  ACCCCTGTCTCCAGAGAGGCCTGAGGACCTGAGAGCCTCTGAGCGCTTCTTGCACTTCATGCTGGGCTGG
  TTTGCACACCCCGTCTTTGTGGATGGAGACTACCCAGCCACCCTGAGGACCCAGATCCAACAGATGAACA
  GACAGTGCTCCCATCCTGTGGCTCAACTCCCCGAGTTCACAGAGGCAGAGAAGCAGCTCCTGAAAGGCTC
  TGCTGATTTTCTGGGTCTGTCGCATTACACCTCCCGCCTCATCAGCAACGCCCCACAAAACACCTGCATC
  CCTAGCTATGATACCATTGGAGGCTTCTCCCAACACGTGAACCATGTGTGGCCCCAGACCTCATCCTCTT
  GGATTCGTGTGGTGCCCTGGGGGATAAGGAGGCTGTTGCAGTTTGTATCCCTGGAATACACAAGAGGAAA
  AGTTCCAATATACCTTGCCGGGAATGGCATGCCCATAGGGGAAAGTGAAAATCTCTTTGATGATTCCTTA
  AGAGTAGACTACTTCAATCAATATATCAATGAGGTGCTCAAGGCTATCAAGGAAGACTCTGTGGATGTTC
  GTTCCTACATTGCTCGTTCCCTCATTGATGGCTTCGAAGGCCCTTCTGGTTACAGCCAGCGGTTTGGCCT
  GCACCACGTCAACTTCAGCGACAGCAGCAAGTCAAGGACTCCCAGGAAATCTGCCTACTTTTTCACTAGC
  ATCATAGAAAAGAACGGTTTCCTCACCAAGGGGGCAAAAAGACTGCTACCACCTAATACAGTAAACCTCC
  CCTCCAAAGTCAGAGCCTTCACTTTTCCATCTGAGGTGCCCTCCAAGGCTAAAGTCGTTTGGGAAAAGTT
  CTCCAGCCAACCCAAGTTCGAAAGAGATTTGTTCTACCACGGGACGTTTCGGGATGACTTTCTGTGGGGC
  GTGTCCTCTTCCGCTTATCAGATTGAAGGCGCGTGGGATGCCGATGGCAAAGGCCCCAGCATCTGGGATA
  ACTTTACCCACACACCAGGGAGCAATGTGAAAGACAATGCCACTGGAGACATCGCCTGTGACAGCTATCA
  CCAGCTGGATGCCGATCTGAATATGCTCCGAGCTTTGAAGGTGAAGGCCTACCGCTTCTCTATCTCCTGG
  TCTCGGATTTTCCCAACTGGGAGAAACAGCTCTATCAACAGTCATGGGGTTGATTATTACAACAGGCTGA
  TCAATGGCTTGGTGGCAAGCAACATCTTTCCCATGGTGACATTGTTCCATTGGGACCTGCCCCAGGCCCT
  CCAGGATATCGGAGGCTGGGAGAATCCTGCCTTGATTGACTTGTTTGACAGCTACGCAGACTTTTGTTTC
  CAGACCTTTGGTGATAGAGTCAAGTTTTGGATGACTTTTAATGAGCCCATGTACCTGGCATGGCTAGGTT
  ATGGCTCAGGGGAATTTCCCCCAGGGGTGAAGGACCCAGGCTGGGCACCATATAGGATAGCCCACGCCGT
  CATCAAAGCCCATGCCAGAGTCTATCACACGTACGATGAGAAATACAGGCAGGAGCAGAAGGGGGTCATC
  TCGCTGAGCCTCAGTACACACTGGGCAGAGCCCAAGTCACCAGGGGTCCCCAGAGATGTGGAAGCCGCTG
  ACCGAATGCTGCAGTTCTCCCTGGGCTGGTTTGCTCACCCCATTTTTAGAAACGGAGACTATCCTGACAC
  CATGAAGTGGAAAGTGGGGAACAGGAGTGAACTGCAGCACTTAGCCACCTCCCGCCTGCCAAGCTTCACT
  GAGGAAGAGAAGAGGTTCATCAGGGCGACGGCCGACGTCTTCTGCCTCAACACGTACTACTCCAGAATCG
  TGCAGCACAAAACACCCAGGCTAAACCCACCCTCCTACGAAGACGACCAGGAGATGGCTGAGGAGGAGGA
  CCCTTCGTGGCCTTCCACGGCAATGAACAGAGCTGCGCCCTGGGGGACGCGAAGGCTGCTGAACTGGATC
  AAGGAAGAGTATGGTGACATCCCCATTTACATCACCGAAAACGGAGTGGGGCTGACCAATCCGAACACGG
  AGGATACTGATAGGATATTTTACCACAAAACCTACATCAATGAGGCTTTGAAAGCCTACAGGCTCGATGG
  TATAGACCTTCGAGGGTATGTCGCCTGGTCTCTGATGGACAACTTTGAGTGGCTAAATGGCTACACGGTC
  AAGTTTGGACTGTACCATGTTGATTTCAACAACACGAACAGGCCTCGCACAGCAAGAGCCTCCGCCAGGT
  ACTACACAGAGGTCATTACCAACAACGGCATGCCACTGGCCAGGGAGGATGAGTTTCTGTACGGACGGTT
  TCCTGAGGGCTTCATCTGGAGTGCAGCTTCTGCTGCATATCAGATTGAAGGTGCGTGGAGAGCAGATGGC
  AAAGGACTCAGCATTTGGGACACGTTTTCTCACACACCACTGAGGGTTGAGAACGATGCCATTGGAGACG
  TGGCCTGTGACAGTTATCACAAGATTGCTGAGGATCTGGTCACCCTGCAGAACCTGGGCGTGTCCCACTA
  CCGTTTTTCCATCTCCTGGTCTCGCATCCTCCCTGATGGAACCACCAGGTACATCAATGAAGCGGGCCTG
  AACTACTACGTGAGGCTCATCGATACACTGCTGGCCGCCAGCATCCAGCCCCAGGTGACCATTTACCACT
  GGGACCTACCACAGACGCTCCAAGATGTAGGAGGCTGGGAGAATGAGACCATCGTGCAGCGGTTTAAGGA
  GTATGCAGATGTGCTCTTCCAGAGGCTGGGAGACAAGGTGAAGTTTTGGATCACGCTGAATGAGCCCTTT
  GTCATTGCTTACCAGGGCTATGGCTACGGAACAGCAGCTCCAGGAGTCTCCAATAGGCCTGGCACTGCCC
  CCTACATTGTTGGCCACAATCTAATAAAGGCTCATGCTGAGGCCTGGCATCTGTACAACGATGTGTACCG
  CGCCAGTCAAGGTGGCGTGATTTCCATCACCATCAGCAGTGACTGGGCTGAACCCAGAGATCCCTCTAAC
  CAGGAGGATGTGGAGGCAGCCAGGAGATATGTTCAGTTCATGGGAGGCTGGTTTGCACATCCTATTTTCA
  AGAATGGAGATTACAATGAGGTGATGAAGACGCGGATCCGTGACAGGAGCTTGGCTGCAGGCCTCAACAA
  GTCTCGGCTGCCAGAATTTACAGAGAGTGAGAAGAGGAGGATCAACGGCACCTATGACTTTTTTGGGTTC
  AATCACTACACCACTGTCCTCGCCTACAACCTCAACTATGCCACTGCCATCTCTTCTTTTGATGCAGACA
  GAGGAGTTGCTTCCATCGCAGATCGCTCGTGGCCAGACTCTGGCTCCTTCTGGCTGAAGATGACGCCTTT
  TGGCTTCAGGAGGATCCTGAACTGGTTAAAGGAGGAATACAATGACCCTCCAATTTATGTCACAGAGAAT
  GGAGTGTCCCAGCGGGAAGAAACAGACCTCAATGACACTGCAAGGATCTACTACCTTCGGACTTACATCA
  ATGAGGCCCTCAAAGCTGTGCAGGACAAGGTGGACCTTCGAGGATACACAGTTTGGAGTGCGATGGACAA
  TTTTGAGTGGGCCACAGGCTTTTCAGAGAGATTTGGTCTGCATTTTGTGAACTACAGTGACCCTTCTCTG
  CCAAGGATCCCCAAAGCATCAGCGAAGTTCTACGCCTCTGTGGTCCGATGCAATGGCTTCCCTGACCCCG
  CTACAGGGCCTCACGCTTGTCTCCACCAGCCAGATGCTGGACCCACCATCAGCCCCGTGAGACAGGAGGA
  GGTGCAGTTCCTGGGGCTAATGCTCGGCACCACAGAAGCACAGACAGCTTTGTACGTTCTCTTTTCTCTT
  GTGCTTCTTGGAGTCTGTGGCTTGGCATTTCTGTCATACAAGTACTGCAAGCGCTCTAAGCAAGGGAAAA
  CACAACGAAGCCAACAGGAATTGAGCCCGGTGTCTTCATTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002299
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002299.3, NP_002290.2
• RefSeq Size: 6288 bp
• RefSeq ORF: 5784 bp
• Locus ID: 3938
• Cytogenetics: 2q21.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Galactose metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are associated with congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood. [provided by RefSeq, Jan 2016]'