MATK (NM_002378) Human Untagged Clone

CAT#: SC303188

MATK (untagged)-Human megakaryocyte-associated tyrosine kinase (MATK), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MATK
• Synonyms: CHK; CTK; HHYLTK; HYL; HYLTK; Lsk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002378, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGGGACATTTCCCTGCTGAACGAAGAGAAGGCAGACCTCGGCGAGGGACCCGTGGTCAGCAGCAGC
  TTCTGGTGAGCCCCCGCTTCCTCCGAGCCTGGCACCCCCCTCCCGTCTCAGCCAGGATGCCAACGAGGCG
  CTGGGCCCCGGGCACCCAGTGTATCACCAAATGCGAGCACACCCGCCCCAAGCCAGGGGAGCTGGCCTTC
  CGCAAGGGCGACGTGGTCACCATCCTGGAGGCCTGCGAGAACAAGAGCTGGTACCGCGTCAAGCACCACA
  CCAGTGGACAGGAGGGGCTGCTGGCAGCTGGGGCGCTGCGGGAGCGGGAGGCCCTCTCCGCAGACCCCAA
  GCTCAGCCTCATGCCGTGGTTCCACGGGAAGATCTCGGGCCAGGAGGCTGTCCAGCAGCTGCAGCCTCCC
  GAGGATGGGCTGTTCCTGGTGCGGGAGTCCGCGCGCCACCCCGGCGACTACGTCCTGTGCGTGAGCTTTG
  GCCGCGACGTCATCCACTACCGCGTGCTGCACCGCGACGGCCACCTCACAATCGATGAGGCCGTGTTCTT
  CTGCAACCTCATGGACATGGTGGAGCATTACAGCAAGGACAAGGGCGCTATCTGCACCAAGCTGGTGAGA
  CCAAAGCGGAAACACGGGACCAAGTCGGCCGAGGAGGAGCTGGCCAGGGCGGGCTGGTTACTGAACCTGC
  AGCATTTGACATTGGGAGCACAGATCGGAGAGGGAGAGTTTGGAGCTGTCCTGCAGGGTGAGTACCTGGG
  GCAAAAGGTGGCCGTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGACGAGACGGCCGTC
  ATGACGAAGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTGCACCAGGGGCTGTACATTG
  TCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTTCTGCGGACCCGGGGTCGAGCCCTCGTGAACAC
  CGCTCAGCTCCTGCAGTTTTCTCTGCACGTGGCCGAGGGCATGGAGTACCTGGAGAGCAAGAAGCTTGTG
  CACCGCGACCTGGCCGCCCGCAACATCCTGGTCTCAGAGGACCTGGTGGCCAAGGTCAGCGACTTTGGCC
  TGGCCAAAGCCGAGCGGAAGGGGCTAGACTCAAGCCGGCTGCCCGTCAAGTGGACGGCGCCCGAGGCTCT
  CAAACACGGGAAGTTCACCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGCTCTGGGAGGTCTTCTCA
  TATGGACGGGCTCCGTACCCTAAAATGTCACTGAAAGAGGTGTCGGAGGCCGTGGAGAAGGGGTACCGCA
  TGGAACCCCCCGAGGGCTGTCCAGGCCCCGTGCACGTCCTCATGAGCAGCTGCTGGGAGGCAGAGCCCGC
  CCGCCGGCCACCCTTCCGCAAACTGGCCGAGAAGCTGGCCCGGGAGCTACGCAGTGCAGGTGCCCCAGCC
  TCCGTCTCAGGGCAGGACGCCGACGGCTCCACCTCGCCCCGAAGCCAGGAGCCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002378
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002378.3, NP_002369.2
• RefSeq Size: 2104 bp
• RefSeq ORF: 1527 bp
• Locus ID: 4145
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Gene Summary: 'The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate region at the 5' end of the coding region and lacks two exons in the coding region, as compared to variant 1. The encoded isoform b has a slightly longer and unique N-terminus compared to isoform a.