Myosin 8 (MYH8) (NM_002472) Human Untagged Clone

CAT#: SC303205

MYH8 (untagged)-Human myosin, heavy chain 8, skeletal muscle, perinatal (MYH8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYH8
• Synonyms: DA7; gtMHC-F; MyHC-peri; MyHC-pn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002472, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGCGAGCTCAGACGCTGAGATGGCTGTTTTTGGCGAAGCTGCTCCCTACCTTCGAAAATCAGAAA
  AGGAGCGGATTGAGGCCCAAAACAAGCCGTTTGATGCTAAAACATCTGTCTTTGTGGCGGAGCCCAAGGA
  ATCCTATGTGAAGAGCACTATACAAAGCAAAGAAGGAGGGAAAGTAACCGTAAAGACTGAAGGTGGAGCA
  ACTCTAACTGTCAGGGAAGACCAAGTCTTCCCTATGAACCCTCCGAAATATGACAAAATTGAGGACATGG
  CCATGATGACTCATCTACACGAGCCTGGAGTGCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGAT
  CTACACCTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAG
  GTGGTGGCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAATG
  CCTATCAGTTCATGTTGACTGATCGAGAGAATCAGTCCATCCTGATCACCGGAGAATCTGGTGCCGGAAA
  GACTGTGAACACCAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAGAAGGAT
  GAATCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGCGCCAATCCCCTACTGGAGGCCTTTG
  GCAATGCCAAAACTGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATTAGAATCCACTTTGGTAC
  TACAGGGAAGCTGGCATCTGCTGATATAGAAACATATCTTTTAGAAAAGTCCAGAGTTACTTTCCAGCTA
  AAGGCGGAAAGAAGCTACCATATTTTTTATCAGATCACTTCCAATAAGAAGCCAGATCTAATTGAAATGC
  TCCTGATCACCACCAACCCATATGACTATGCCTTCGTCAGTCAGGGGGAGATCACAGTTCCCAGTATTGA
  TGACCAAGAAGAGTTGATGGCCACTGATAGTGCCATTGACATCCTGGGCTTCACTCCTGAAGAGAAAGTG
  TCCATCTATAAACTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAAAAGCAGCGTGAGG
  AGCAAGCTGAGCCAGATGGCACAGAAGTCGCTGACAAGGCAGCCTATCTCCAGAGTCTGAACTCTGCAGA
  CCTACTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTTGGCAATGAGTATGTCACCAAAGGCCAGACTGTG
  CAGCAGGTGTACAATGCGGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGGATGGTCA
  CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGG
  CTTTGAAATCTTTGATTTTAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG
  TTTTTCAACCACCACATGTTTGTGCTAGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCA
  TTGACTTTGGGATGGACCTGGCTGCCTGCATTGAGCTCATTGAGAAGCCACTGGGCATCTTCTCCATCCT
  GGAAGAGGAGTGCATGTTCCCTAAGGCAACGGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTG
  GGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAGGCTGAGGCCCACTTCTCTCTGATTC
  ACTATGCTGGCACTGTGGACTACAACATTACTGGCTGGCTGGACAAAAATAAGGACCCCCTGAATGATAC
  TGTGGTTGGGCTGTACCAGAAGTCTGCAATGAAGACTCTAGCCAGTCTCTTTTCCACGTATGCTAGTGCT
  GAAGCAGATAGCAGCGCGAAGAAAGGTGCTAAGAAAAAGGGCTCTTCTTTCCAGACTGTGTCTGCCCTTT
  TCAGGGAAAATTTAAATAAATTGATGACGAATCTGAGGAGCACACACCCTCACTTCGTACGGTGTATCAT
  TCCCAATGAAACCAAAACTCCTGGGGCAATGGAACATGAACTTGTGTTGCACCAGCTGAGGTGTAATGGT
  GTGCTGGAAGGCATCCGCATCTGTAGGAAAGGATTCCCAAGCAGAATCTTATATGGTGATTTCAAACAAA
  GATACAAGGTTTTAAATGCAAGTGCTATTCCAGAGGGACAGTTCATTGACAGCAAGAAGGCTTCTGAGAA
  ACTTCTTGCATCTATTGATATTGATCATACTCAATATAAATTTGGACATACCAAGGTTTTCTTCAAAGCT
  GGACTTCTGGGTCTTCTGGAAGAAATGAGAGATGAAAAATTAGCCCAAATTATAACAAGAACACAAGCTG
  TCTGTAGGGGATTCCTAATGAGGGTAGAATATCAGAAGATGTTGCAAAGGAGAGAAGCACTTTTCTGCAT
  CCAGTATAATGTCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTTTTCAAGATTAAG
  CCCCTCCTCAAGAGTGCAGAGACCGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAGAAAACCAAAG
  ATGAACTCGCCAAGTCAGAGGCAAAACGGAAGGAGCTAGAGGAAAAAATGGTCACTCTCTTAAAAGAGAA
  AAATGACCTGCAACTCCAGGTTCAATCTGAAGCAGATAGCTTGGCTGATGCAGAGGAAAGGTGTGAGCAA
  CTGATTAAAAACAAAATCCAACTTGAGGCCAAAATCAAAGAGGTGACTGAAAGAGCTGAGGAGGAGGAAG
  AGATCAATGCTGAGCTGACAGCCAAGAAGAGAAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACAT
  TGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAACATGCCACGGAGAACAAGGTGAAAAAT
  CTTACAGAAGAGATGGCAGGCCTGGATGAAACCATTGCAAAACTGTCCAAGGAGAAGAAGGCTCTCCAAG
  AGACCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACATCCTGACCAAAGCTAA
  AACCAAGCTAGAACAGCAAGTGGATGATCTTGAAGGGTCTCTGGAACAAGAAAAGAAGCTTCGAATGGAT
  CTAGAAAGAGCAAAGCGGAAACTGGAGGGTGACCTCAAATTGGCCCAAGAATCCACAATGGATATGGAAA
  ATGACAAACAGCAACTTGATGAAAAGCTTGAAAAGAAAGAATTTGAAATCAGCAATTTGATAAGCAAAAT
  TGAAGATGAGCAAGCTGTAGAAATTCAACTACAGAAGAAGATCAAAGAGTTGCAGGCCCGCATTGAGGAG
  CTGGGGGAAGAAATCGAGGCAGAGAGGGCGTCCCGAGCCAAAGCGGAGAAGCAGCGCTCTGACCTCTCCC
  GGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCAACTTCTGCTCAGGTGGAATTGAA
  CAAGAAGCGGGAGGCTGAGTTTCAGAAACTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCATGAAGCT
  ATGGTGGCTGCTCTTCGGAAGAAGCACGCAGACAGTATGGCTGAGCTTGGGGAGCAGATTGACAACTTGC
  AGCGGGTCAAACAGAAGCTGGAGAAGGAGAAGAGTGAGCTGAAGATGGAGACTGATGACCTCAGCAGTAA
  CGCAGAGGCCATTTCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCTCTCTAGAAGATCAAGTGAGT
  GAGCTTAAGACCAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCCTGC
  AGACAGAAGCGGGTGAATATTCTCGACAATTAGATGAGAAAGATGCTTTAGTCTCTCAGCTTTCAAGGAG
  CAAGCAAGCATCTACTCAGCAGATTGAAGAGCTGAAACATCAACTAGAGGAAGAAACTAAAGCCAAGAAC
  GCCCTGGCACACGCCCTGCAGTCCTCCCGCCATGACTGCGACCTGCTGCGGGAACAGTATGAGGAAGAGC
  AGGAAGGCAAAGCTGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGAACCAA
  ATACGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAAAAGTTGGCCCAGCGCCTG
  CAAGAAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGCGGC
  TCCAGAATGAAGTTGAAGACCTCATGCTTGATGTGGAAAGGTCTAATGCAGCCTGTGCAGCCCTTGATAA
  GAAGCAAAGGAACTTTGACAAGGTCCTATCAGAATGGAAGCAGAAGTATGAGGAAACTCAGGCTGAACTT
  GAGGCCTCCCAGAAGGAGTCACGTTCTCTTAGCACTGAGCTGTTCAAGGTGAAGAATGTCTATGAGGAAT
  CCCTGGATCAACTCGAAACGCTAAGAAGAGAAAATAAGAACTTGCAACAGGAGATTTCTGACCTCACTGA
  GCAGATTGCAGAGGGAGGAAAGCAAATTCATGAATTGGAGAAAATAAAGAAGCAAGTAGAACAAGAGAAA
  TGTGAAATTCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTTGAACATGAAGAAGGAAAGATTCTGCGTA
  TCCAGCTTGAGTTAAACCAAGTCAAGTCTGAAGTTGATAGAAAAATCGCAGAAAAGGATGAGGAAATTGA
  CCAGCTGAAGAGAAACCACACTAGAGTCGTGGAGACAATGCAGAGCACGCTGGATGCAGAGATTAGAAGC
  AGAAATGATGCTCTGAGAGTCAAGAAGAAAATGGAAGGAGATCTGAATGAAATGGAAATCCAGCTGAACC
  ATGCCAATCGCTTAGCTGCAGAGAGTTTAAGGAACTACAGGAACACCCAAGGAATCCTGAAGGAAACCCA
  GCTCCACCTGGATGATGCTCTCCGGGGCCAGGAGGACCTCAAGGAACAGCTGGCAATTGTGGAGCGCAGA
  GCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGTGGGCCACTCTGGAACAGACAGAGAGAAGCAGGAAAA
  TCGCCGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTCCAGCTCCTCCACACCCAGAATACCAGTCTCAT
  TAACACCAAGAAGAAATTAGAAAATGACGTTTCCCAACTCCAAAGTGAAGTGGAAGAAGTAATCCAAGAA
  TCACGCAATGCAGAAGAGAAAGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAGGAGCTGAAGA
  AGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCA
  GCATCGTCTAGATGAGGCCGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAGAAACTGGAGGCC
  AGGGTACGTGAGCTTGAAGGAGAGGTTGAAAATGAACAGAAACGTAATGCAGAGGCTGTTAAAGGTTTAC
  GGAAACATGAGCGACGAGTAAAAGAACTCACCTACCAGACTGAAGAAGATCGCAAGAATGTTCTCAGGCT
  GCAGGACTTGGTAGATAAATTACAGGCGAAGGTGAAATCATACAAGAGACAAGCTGAGGAGGCTGAGGAA
  CAATCCAATGCTAATCTATCTAAATTCCGCAAACTCCAGCATGAGCTGGAGGAGGCCGAGGAACGGGCTG
  ACATTGCTGAGTCCCAGGTCAACAAATTGCGAGTGAAGAGCCGAGAGGTTCACACAAAAATCAGTGCAGA
  GTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002472
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002472.2, NP_002463.2
• RefSeq Size: 6038 bp
• RefSeq ORF: 5814 bp
• Locus ID: 4626
• Cytogenetics: 17p13.1
• Protein Pathways: Tight junction, Viral myocarditis
• Gene Summary: 'Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009]'