PER1 (NM_002616) Human Untagged Clone

CAT#: SC303227

PER1 (untagged)-Human period homolog 1 (Drosophila) (PER1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PER1
• Synonyms: hPER; PER; RIGUI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002616, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGGCCCCCTAGAAGGGGCTGATGGGGGAGGGGACCCCAGGCCTGGGGAATCATTTTGTCCTGGGG
  GCGTCCCATCCCCTGGGCCCCCACAGCACCGGCCTTGCCCAGGCCCCAGCCTGGCCGATGACACCGATGC
  CAACAGCAATGGTTCAAGTGGCAATGAGTCCAACGGGCATGAGTCTAGAGGCGCATCTCAGCGGAGCTCA
  CACAGCTCCTCCTCAGGCAACGGCAAGGACTCAGCCCTGCTGGAGACCACTGAGAGCAGCAAGAGCACAA
  ACTCTCAGAGCCCATCCCCACCCAGCAGTTCCATTGCCTACAGCCTCCTGAGTGCCAGCTCAGAGCAGGA
  CAACCCGTCCACCAGTGGCTGCAGCAGTGAACAGTCAGCCCGGGCAAGGACTCAGAAGGAACTCATGACA
  GCACTTCGAGAGCTCAAGCTTCGACTGCCGCCAGAGCGCCGGGGCAAGGGCCGCTCTGGGACCCTGGCCA
  CGCTGCAGTACGCACTGGCCTGTGTCAAGCAGGTGCAGGCCAACCAGGAATACTACCAGCAGTGGAGCCT
  GGAGGAGGGCGAGCCTTGCTCCATGGACATGTCCACCTATACCCTGGAGGAGCTGGAGCACATCACGTCT
  GAGTACACACTTCAGAACCAGGATACCTTCTCAGTGGCTGTCTCCTTCCTGACGGGCCGAATCGTCTACA
  TTTCGGAGCAGGCAGCCGTCCTGCTGCGTTGCAAGCGGGACGTGTTCCGGGGTACCCGCTTCTCTGAGCT
  CCTGGCTCCCCAGGATGTGGGAGTCTTCTATGGTTCCACTGCTCCATCTCGCCTGCCCACCTGGGGCACA
  GGGGCCTCAGCAGGTTCAGGCCTCAGGGACTTTACCCAGGAGAAGTCCGTCTTCTGCCGTATCAGAGGAG
  GTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCGTATGTGACCAAGATCCGGGT
  CTCAGATGGGGCCCCTGCACAGCCGTGCTGCCTGCTGATTGCAGAGCGCATCCATTCGGGTTACGAAGCT
  CCCCGGATACCCCCTGACAAGAGGATTTTCACTACGCGGCACACACCCAGCTGCCTCTTCCAGGATGTGG
  ATGAAAGGGCTGCCCCCCTGCTGGGCTACCTGCCCCAGGACCTCCTGGGGGCCCCAGTGCTCCTGTTCCT
  GCATCCTGAGGACCGACCCCTCATGCTGGCTATCCACAAGAAGATTCTGCAGTTGGCGGGCCAGCCCTTT
  GACCACTCCCCTATCCGCTTCTGTGCCCGCAACGGGGAGTATGTCACCATGGACACCAGCTGGGCTGGCT
  TTGTGCACCCCTGGAGCCGCAAGGTAGCCTTCGTGTTGGGCCGCCACAAAGTACGCACGGCCCCCCTGAA
  TGAGGACGTGTTCACTCCCCCGGCCCCCAGCCCAGCTCCCTCCCTGGACACTGATATCCAGGAGCTGTCA
  GAGCAGATCCACCGGCTGCTGCTGCAGCCCGTCCACAGCCCCAGCCCCACGGGACTCTGTGGAGTCGGCG
  CCGTGACATCCCCAGGCCCTCTCCACAGCCCTGGGTCCTCCAGTGATAGCAACGGGGGTGATGCAGAGGG
  GCCTGGGCCTCCTGCGCCAGTGACTTTCCAGCAGATCTGTAAGGATGTGCATCTGGTGAAGCACCAGGGC
  CAGCAGCTTTTTATTGAGTCTCGGGCCCGGCCTCAGTCCCGGCCCCGCCTCCCTGCTACAGGCACGTTCA
  AGGCCAAGGCCCTTCCCTGCCAATCCCCAGACCCAGAGCTGGAGGCGGGTTCTGCTCCCGTCCAGGCCCC
  ACTAGCCTTGGTCCCTGAGGAGGCCGAGAGGAAAGAAGCCTCCAGCTGCTCCTACCAGCAGATCAACTGC
  CTGGACAGCATCCTCAGGTACCTGGAGAGCTGCAACCTCCCCAGCACCACTAAGCGTAAATGTGCCTCCT
  CCTCCTCCTATACCACCTCCTCAGCCTCTGACGACGACAGGCAGAGGACAGGTCCAGTCTCTGTGGGGAC
  CAAGAAAGATCCGCCGTCAGCAGCGCTGTCTGGGGAGGGGGCCACCCCACGGAAGGAGCCAGTGGTGGGA
  GGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCGGAGAGTGTGGTGTCCGTCACCAGTCAGTGTAGCT
  TCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCCCCGGAGTCGGACATCATCATGATGGAGGACCT
  GCCTGGCCTAGCCCCAGGCCCAGCCCCCAGCCCAGCCCCCAGCCCCACAGTAGCCCCTGACCCAGCCCCA
  GACGCCTACCGTCCAGTGGGGCTGACCAAGGCCGTGCTGTCCCTGCACACACAGAAGGAAGAGCAAGCCT
  TCCTCAGCCGCTTCCGAGACCTGGGCAGGCTGCGTGGACTCGACAGCTCTTCCACAGCTCCCTCAGCCCT
  TGGCGAGCGAGGCTGCCACCACGGCCCCGCACCCCCAAGCCGCCGACACCACTGCCGATCCAAAGCCAAG
  CGCTCACGCCACCACCAGAACCCTCGGGCTGAAGCGCCCTGCTATGTCTCACACCCCTCACCCGTGCCAC
  CCTCCACCCCCTGGCCCACCCCACCAGCCACTACCCCCTTCCCAGCGGTTGTCCAGCCCTACCCTCTCCC
  AGTGTTCTCTCCTCGAGGAGGCCCCCAGCCTCTTCCCCCTGCTCCCACATCTGTGCCCCCAGCTGCTTTC
  CCCGCCCCTTTGGTGACCCCAATGGTGGCCTTGGTGCTCCCTAACTATCTGTTCCCAACCCCATCCAGCT
  ATCCTTATGGGGCACTCCAGACCCCTGCTGAAGGGCCTCCCACTCCTGCCTCGCACTCCCCTTCTCCATC
  CTTGCCCGCCCTCGCCCCGAGTCCTCCTCACCGCCCGGACTCTCCACTGTTCAACTCGAGATGCAGCTCT
  CCACTCCAGCTCAATCTGCTGCAGCTGGAGGAGCTCCCCCGTGCTGAGGGGGCTGCTGTTGCAGGAGGCC
  CTGGGAGCAGTGCCGGGCCCCCACCTCCCAGTGCGGAGGCTGCTGAGCCAGAGGCCAGACTGGCGGAGGT
  CACTGAGTCCTCCAATCAGGACGCACTTTCCGGCTCCAGTGACCTGCTCGAACTTCTGCTGCAAGAGGAC
  TCGCGCTCCGGCACAGGCTCCGCAGCCTCGGGCTCCTTGGGCTCTGGCTTGGGCTCTGGGTCTGGTTCAG
  GCTCCCATGAAGGGGGCAGCACCTCAGCCAGCATCACTCGCAGCAGCCAGAGCAGCCACACAAGCAAATA
  CTTTGGCAGCATCGACTCTTCCGAGGCTGAGGCTGGGGCTGCTCGGGGCGGGGCTGAGCCTGGGGACCAG
  GTGATTAAGTACGTGCTCCAGGATCCCATTTGGCTGCTCATGGCCAATGCTGACCAGCGCGTCATGATGA
  CCTACCAGGTGCCCTCCAGGGACATGACCTCTGTGCTGAAGCAGGATCGGGAGCGGCTCCGAGCCATGCA
  GAAGCAGCAGCCTCGGTTTTCTGAGGACCAGCGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAG
  GGCCAACTGCCTCGGGCTCTTGATGTGATGGCCTGTGTGGACTGTGGGAGCAGCACCCAAGATCCTGGTC
  ACCCTGATGACCCACTCTTCTCAGAGCTGGATGGACTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGA
  GCAGGGCAGCAGCGGTGGCGGCAGTGGTGAGGGAGAGGGCTGCGAGGAGGCCCAAGGCGGGGCCAAGGCT
  TCAAGCTCTCAGGACTTGGCTATGGAGGAGGAGGAAGAAGGCAGGAGCTCATCCAGTCCAGCCTTACCTA
  CAGCAGGAAACTGCACCAGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002616
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002616.2, NP_002607.2
• RefSeq Size: 4717 bp
• RefSeq ORF: 3873 bp
• Locus ID: 5187
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: 'This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]'