GDF 9 (GDF9) (NM_005260) Human Untagged Clone

CAT#: SC303612

GDF9 (untagged)-Human growth differentiation factor 9 (GDF9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GDF9
• Synonyms: POF14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005260, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCACGTCCCAACAAATTCCTCCTTTGGTTTTGCTGCTTTGCCTGGCTGTGTTTTCCTATTAGCCTTG
  GTTCTCAGGCTTCTGGGGGAGAAGCTCAGATTGCTGCTAGTGCTGAGTTGGAATCTGGGGCTATGCCTTG
  GTCCTTGCTGCAGCATATAGATGAGAGAGACAGAGCTGGCCTCCTTCCCGCGCTTTTCAAAGTTCTATCT
  GTTGGGCGAGGTGGGTCACCTAGGCTGCAGCCAGACTCCAGAGCTTTGCACTACATGAAGAAGCTCTATA
  AGACATATGCTACCAAGGAAGGGATTCCTAAATCCAATAGAAGTCACCTCTACAACACTGTTCGGCTCTT
  CACCCCCTGTACCCGGCACAAGCAGGCTCCTGGAGACCAGGTAACAGGAATCCTTCCATCAGTGGAACTG
  CTATTTAACCTGGATCGCATTACTACCGTTGAACACTTACTCAAGTCAGTCTTGCTGTACAATATCAACA
  ACTCAGTTTCTTTTTCCTCTGCTGTCAAATGTGTGTGCAATCTAATGATAAAGGAGCCAAAGTCTTCTAG
  CAGGACTCTCGGCAGAGCTCCATACTCATTTACCTTTAACTCACAGTTTGAATTTGGAAAGAAACACAAA
  TGGATTCAGATTGATGTGACCAGCCTCCTTCAACCTTTAGTGGCCTCCAACAAGAGAAGTATTCACATGT
  CTATAAATTTTACTTGCATGAAAGACCAGCTGGAGCATCCTTCAGCACAGAATGGTTTGTTTAACATGAC
  TCTGGTGTCCCCCTCACTGATCTTATATTTGAATGACACAAGTGCTCAGGCTTATCACAGCTGGTATTCC
  CTTCACTATAAAAGGAGGCCTTCCCAGGGTCCTGACCAGGAGAGAAGTCTGTCTGCCTATCCTGTGGGAG
  AAGAGGCTGCTGAGGATGGGAGATCTTCCCATCACCGTCACCGCAGAGGTCAGGAAACTGTCAGTTCTGA
  ATTGAAGAAGCCCTTGGGCCCAGCTTCCTTCAATCTGAGTGAATACTTCAGACAATTTCTTCTTCCCCAA
  AATGAGTGTGAGCTCCATGACTTTAGACTTAGCTTTAGTCAGCTGAAGTGGGACAACTGGATTGTGGCTC
  CGCACAGGTACAACCCTCGATACTGTAAAGGGGACTGTCCAAGGGCAGTTGGACATCGGTATGGCTCTCC
  AGTTCACACCATGGTACAGAACATCATCTATGAGAAGCTGGACTCCTCAGTGCCAAGACCGTCATGTGTA
  CCTGCCAAATACAGCCCCTTGAGTGTTTTGACCATTGAGCCCGATGGCTCAATTGCCTATAAAGAGTACG
  AAGATATGATAGCTACAAAGTGCACCTGTCGTTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005260
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005260.5, NP_005251.1
• RefSeq Size: 2607 bp
• RefSeq ORF: 1365 bp
• Locus ID: 2661
• Cytogenetics: 5q31.1
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell - Pluripotency, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
• Gene Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).