RP1 (NM_006269) Human Untagged Clone

CAT#: SC303763

RP1 (untagged)-Human retinitis pigmentosa 1 (autosomal dominant) (RP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RP1
• Synonyms: DCDC4A; ORP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006269, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTGATACCCCTTCTACTGGTTTTTCCATCATTCATCCTACGTCTTCTGAAGGTCAAGTTCCACCCC
  CTCGCCATTTGAGCCTCACTCATCCTGTTGTGGCCAAGCGAATCAGTTTCTACAAGAGCGGAGACCCCCA
  ATTCGGCGGGGTCAGGGTGGTGGTCAACCCTCGCTCCTTTAAGTCCTTTGATGCTCTGCTGGATAACTTG
  TCCAGGAAGGTGCCCCTCCCTTTTGGAGTGAGGAACATCAGCACCCCTCGGGGCAGGCACAGCATCACGC
  GCCTGGAGGAGCTGGAGGACGGCGAGTCCTACCTATGTTCCCACGGCAGGAAGGTGCAGCCTGTAGACCT
  GGACAAAGCCCGTCGGCGCCCGCGGCCCTGGCTCAGCAGCCGGGCCATTAGCGCGCACTCACCGCCCCAC
  CCCGTAGCCGTCGCTGCTCCCGGCATGCCCCGCCCCCCACGGAGCCTAGTGGTCTTCAGGAATGGCGACC
  CGAAGACGAGGCGTGCGGTTCTTCTGAGCAGGAGGGTCACCCAGAGCTTCGAGGCATTTCTACAGCACCT
  GACAGAGGTCATGCAGCGCCCTGTGGTCAAGCTGTACGCTACGGACGGAAGGAGGGTTCCCAGCCTCCAG
  GCAGTGATCCTGAGCTCTGGAGCTGTGGTGGCGGCAGGAAGGGAGCCATTTAAACCAGGAAATTATGACA
  TCCAAAAATACTTGCTTCCTGCTAGATTACCAGGGATCTCTCAGCGTGTGTACCCCAAGGGAAATGCAAA
  GTCAGAAAGCAGAAAGATAAGCACACATATGTCTTCAAGCTCAAGGTCCCAGATTTATTCTGTTTCTTCT
  GAGAAAACACATAATAATGATTGCTACTTAGACTATTCTTTTGTTCCTGAAAAGTACTTGGCCTTAGAAA
  AGAATGATTCTCAGAATTTACCAATATATCCTTCTGAAGATGATATTGAGAAATCAATTATTTTTAATCA
  AGACGGCACTATGACAGTTGAGATGAAAGTTCGATTCAGAATAAAAGAGGAAGAAACCATAAAATGGACA
  ACTACTGTCAGTAAAACTGGTCCTTCTAATAATGATGAAAAGAGTGAGATGAGTTTTCCAGGAAGAACAG
  AAAGTCGATCATCTGGTTTAAAGCTTGCAGCATGTTCATTCTCTGCAGATGTGTCACCTATGGAGCGAAG
  CAGTAATCAAGAGGGCAGTTTGGCAGAGGAGATAAACATTCAAATGACAGATCAAGTGGCTGAAACTTGC
  AGTTCTGCTAGTTGGGAGAATGCTACTGTGGACACAGATATCATCCAGGGAACTCAAGACCAAGCAAAGC
  ATCGTTTTTATAGGCCCCCTACACCTGGACTAAGAAGAGTGAGACAAAAGAAATCTGTGATTGGCAGTGT
  GACCTTAGTATCTGAAACTGAGGTTCAAGAGAAAATGATTGGACAGTTTTCATATAGTGAAGAAAGGGAA
  AGTGGGGAAAACAAGTCTGAGTATCACATGTTTACACATTCTTGCAGTAAAATGTCATCAGTATCTAACA
  AACCAGTACTTGTTCAGATCAATAACAATGATCAAATGGAGGAGTCATCATTAGAAAGAAAAAAGGAAAA
  CAGTCTGCTTAAGTCAAGTGCAATAAGTGCTGGTGTTATAGAAATTACAAGTCAGAAGATGTTAGAGATG
  TCACATAATAATGGTTTGCCATCAACTATATCAAATAACTCAATTGTGGAGGAAGATGTAGTTGATTGTG
  TGGTATTGGACAACAAAACTGGTATCAAGAACTTCAAAACTTATGGTAACACCAATGATAGGTTCAGTCC
  TATTTCAGCAGATGCAACCCATTTTTCAAGTAATAACTCTGGAACTGACAAAAATATTTCTGAGGCTCCA
  GCTTCAGAAGCATCCTCTACTGTCACTGCAAGAATTGACAGACTAATTAATGAATTTGCTCAGTGTGGTT
  TAACAAAACTTCCAAAAAATGAAAAGAAGATTTTGTCATCTGTTGCCAGCAAAAAGAAGAAAAAATCTCG
  ACAGCAAGCAATAAATTCCAGGTATCAAGATGGACAGCTTGCAACCAAAGGAATTCTTAATAAGAATGAG
  AGAATAAACACAAAAGGTAGAATTACAAAGGAAATGATAGTGCAAGATTCAGATAGTCCCCTTAAAGGAG
  GGATACTTTGTGAGGAAGACCTCCAGAAAAGTGATACTGTAATTGAATCAAATACTTTTTGTTCCAAAAG
  TAATCTCAATTCCACGATTTCCAAGAATTTCCATAGAAATAAATTAAATACTACTCAAAATTCCAAGGTT
  CAAGGACTTTTAACCAAAAGAAAATCTAGATCACTAAATAAAATAAGCTTAGGAGCACCTAAAAAAAGAG
  AAATCGGTCAAAGAGATAAAGTGTTTCCTCACAATGAATCTAAATATTGCAAAAGTACTTTTGAAAACAA
  AAGTTTATTTCATGTATTTAACATCCTTGAGCAAAAACCCAAAGATTTTTATGCACCGCAATCTCAAGCA
  GAAGTGGCATCTGGGTATTTGAGAGGAATGGCAAAGAAGAGTTTAGTTTCAAAAGTTACTGATTCACACA
  TAACTTTAAAAAGCCAGAAAAAACGTAAAGGGGATAAAGTGAAAGCAAGTGCTATTTTAAGTAAACAACA
  TGCTACAACCAGGGCAAATTCTTTAGCTTCTTTGAAAAAACCTGATTTTCCTGAGGCTATTGCTCATCAT
  TCAATTCAAAATTATATACAGAGTTGGTTGCAGAACATAAATCCATATCCAACTTTAAAGCCTATAAAAT
  CAGCTCCAGTATGTAGAAATGAAACGAGTGTGGTAAATTGTAGCAATAATAGTTTTTCAGGGAATGATCC
  CCATACAAATTCTGGAAAAATAAGTAATTTTGTTATGGAAAGTAATAAGCACATAACTAAAATTGCCGGT
  TTGACAGGAGATAATCTATGTAAAGAGGGAGATAAGTCTTTTATTGCCAATGACACTGGTGAAGAAGATC
  TCCATGAGACACAGGTTGGATCTCTGAATGATGCTTATTTGGTTCCCCTGCATGAACACTGTACTTTGTC
  ACAGTCAGCTATTAATGATCATAATACTAAAAGTCATATAGCTGCTGAAAAATCAGGACCAGAGAAAAAA
  CTTGTTTACCAGGAAATAAACCTAGCTAGAAAAAGGCAAAGTGTAGAGGCTGCCATTCAAGTAGATCCTA
  TAGAAGAGGAAACTCCAAAAGACCTCTTACCAGTCCTGATGCTTCACCAATTGCAAGCTTCAGTTCCTGG
  TATTCACAAGACTCAGAATGGAGTTGTTCAAATGCCAGGTTCACTTGCAGGTGTTCCCTTTCATTCTGCA
  ATATGTAATTCATCCACTAATCTCCTTCTAGCTTGGCTCTTGGTGCTAAACCTAAAGGGAAGTATGAATA
  GCTTCTGTCAAGTTGATGCTCACAAGGCTACCAACAAATCTTCAGAAACACTTGCATTGTTGGAGATTCT
  AAAGCACATAGCTATCACAGAGGAAGCTGATGACTTGAAAGCTGCTGTTGCCAATTTAGTGGAGTCAACT
  ACAAGCCACTTTGGACTCAGTGAGAAAGAACAAGACATGGTTCCAATAGATCTTTCTGCAAATTGTTCCA
  CGGTCAACATTCAGAGTGTTCCTAAGTGCAGTGAAAATGAAAGAACACAAGGAATCTCCTCTTTGGATGG
  AGGTTGCTCTGCCAGTGAGGCATGTGCCCCTGAAGTCTGTGTTTTGGAAGTGACTTGCTCTCCATGTGAG
  ATGTGCACTGTAAATAAGGCTTATTCTCCAAAAGAGACATGTAACCCCAGTGACACTTTTTTTCCTAGTG
  ATGGTTATGGTGTGGATCAGACTTCTATGAATAAGGCTTGTTTCCTAGGAGAGGTCTGTTCACTTACTGA
  TACTGTGTTTTCTGATAAGGCTTGTGCTCAAAAGGAGAACCATACCTATGAGGGAGCTTGCCCAATTGAT
  GAGACCTACGTTCCTGTCAATGTCTGCAATACCATTGACTTTTTAAACTCCAAAGAAAACACATATACTG
  ATAACTTGGATTCAACTGAAGAGTTAGAAAGAGGTGATGACATTCAGAAAGATCTAAATATTTTGACAGA
  CCCTGAATATAAAAATGGATTTAATACATTGGTGTCACATCAAAATGTCAGTAATTTAAGCTCCTGTGGC
  CTTTGCCTAAGTGAAAAAGAAGCAGAACTTGATAAGAAACATAGTTCTCTAGATGATTTTGAAAATTGTT
  CACTAAGGAAGTTTCAGGATGAAAATGCATATACTTCCTTTGATATGGAAGAACCACGGACTTCTGAAGA
  ACCAGGCTCAATAACCAACAGCATGACATCAAGTGAAAGAAACATTTCAGAATTGGAATCTTTTGAAGAA
  TTAGAAAACCATGACACTGATATCTTTAATACAGTGGTAAATGGAGGAGAGCAAGCCACTGAAGAATTAA
  TCCAAGAAGAGGTAGAGGCTAGTAAAACTTTAGAATTGATAGACATCTCTAGTAAGAATATTATGGAAGA
  AAAAAGAATGAACGGTATAATTTATGAAATAATCAGTAAGAGGCTGGCAACACCACCATCTTTAGATTTT
  TGCTATGATTCTAAGCAAAATAGTGAAAAGGAGACCAATGAAGGAGAAACTAAGATGGTAAAAATGATGG
  TGAAAACTATGGAAACTGGAAGTTATTCAGAGTCCTCTCCTGATTTAAAAAAATGCATCAAAAGTCCAGT
  GACTTCTGATTGGTCAGACTATCGGCCTGACAGTGACAGTGAGCAGCCATATAAAACATCCAGTGATGAT
  CCCAATGACAGTGGCGAACTTACCCAAGAGAAAGAATATAACATAGGATTTGTTAAAAGGGCAATAGAAA
  AACTGTACGGTAAAGCAGATATTATCAAACCATCTTTTTTTCCTGGGTCTACCCGCAAATCTCAGGTTTG
  TCCTTATAATTCTGTGGAATTTCAGTGTTCCAGGAAAGCAAGTCTTTATGATTCTGAAGGGCAGTCATTT
  GGCTCTTCTGAACAGGTATCTAGTAGTTCATCTATGTTGCAGGAATTCCAGGAGGAAAGACAAGATAAGT
  GTGATGTTAGTGCTGTGAGGGACAATTATTGTAGGGGTGACATTGTAGAACCTGGTACAAAACAAAATGA
  TGATAGCAGAATCCTCACAGACATAGAGGAAGGAGTACTGATTGACAAAGGCAAATGGCTTCTGAAAGAA
  AATCATTTGCTAAGGATGTCATCTGAAAATCCTGGCATGTGTGGCAATGCAGACACCACATCAGTGGACA
  CCCTACTTGATAATAACAGCAGTGAGGTACCATATTCACATTTTGGTAATTTGGCCCCAGGCCCAACGAT
  GGATGAACTCTCCTCTTCAGAACTCGAGGAACTGACTCAACCCCTTGAACTAAAATGCAATTACTTTAAC
  ATGCCTCATGGTAGTGACTCAGAACCTTTTCATGAGGACTTGCTGGATGTTCGCAATGAAACCTGTGCCA
  AGGAAAGAATAGCAAATCATCATACAGAGGAGAAGGGTAGTCATCAGTCAGAAAGAGTATGCACATCTGT
  CACTCATTCCTTTATTTCTGCTGGTAACAAAGTCTACCCTGTCTCTGATGATGCTATTAAAAACCAACCA
  TTGCCTGGCAGTAATATGATTCATGGTACACTTCAGGAAGCTGACTCTTTGGATAAACTGTATGCTCTTT
  GTGGTCAACATTGCCCAATACTAACTGTTATTATCCAACCCATGAATGAGGAAGACCGAGGATTTGCATA
  TCGCAAAGAATCTGATATTGAAAATTTCTTGGGTTTTTATTTATGGATGAAAATACACCCATATTTACTT
  CAGACAGACAAAAATGTGTTCAGGGAAGAGAACAATAAAGCAAGTATGAGACAAAATCTTATTGATAATG
  CCATTGGTGATATATTTGATCAGTTTTATTTCAGTAACACATTTGACTTGATGGGTAAAAGAAGAAAACA
  AAAAAGAATTAACTTCTTGGGGTTAGAGGAAGAAGGTAATTTAAAGAAATTTCAACCAGATTTGAAGGAA
  AGGTTTTGTATGAATTTCTTGCACACATCATTGTTAGTTGTGGGTAATGTGGATTCAAATACACAAGACC
  TCAGCGGTCAGACAAATGAAATCTTTAAAGCAGTCGATGAGAATAACAACTTATTAAATAACAGATTCCA
  GGGCTCAAGAACAAATCTCAACCAAGTAGTAAGAGAAAATATCAACTGTCATTACTTCTTTGAAATGCTT
  GGTCAAGCTTGCCTCTTAGATATTTGCCAAGTTGAGACCTCCTTAAATATTAGCAACAGAAATATTTTAG
  AACTTTGTATGTTTGAGGGTGAAAATCTTTTCATTTGGGAAGAGGAAGACATATTAAATTTAACTGATCT
  TGAAAGCAGTAGAGAACAAGAAGATTTATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006269
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006269.1, NP_006260.1
• RefSeq Size: 7100 bp
• RefSeq ORF: 6471 bp
• Locus ID: 6101
• Cytogenetics: 8q11.23-q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. Transcript variants resulted from an alternative promoter and alternative splicings have been found, which overlap the current reference sequence and has several exons upstream and downstream of the current reference sequence. However, the biological validity and full-length nature of some variants cannot be determined at this time.[provided by RefSeq, Sep 2010]'