TAF5 (NM_006951) Human Untagged Clone

CAT#: SC303858

TAF5 (untagged)-Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF5
• Synonyms: TAF(II)100; TAF2D; TAFII-100; TAFII100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC303858 ORF sequence for NM_006951, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGCTGGCGGAGGAGCAGACGGAGGTGGCGGTCAAGCTAGAGCCTGAGGGACCGCCAACGCTGC
  TACCTCCGCAGGCGGGGGACGGCGCAGGCGAGGGTAGCGGCGGCACTACCAACAACGGCCCCAACGGCGG
  CGGCGGGAACGTTGCGGCGTCGTCGTCCACTGGCGGGGATGGCGGGACCCCCAAGCCCACGGTGGCTGTC
  TCCGCCGCTGCCCCGGCGGGGGCGGCCCCGGTGCCCGCCGCTGCTCCGGACGCCGGCGCTCCGCATGACC
  GACAGACTCTACTGGCCGTGCTGCAGTTCCTACGGCAGAGCAAACTCCGCGAGGCCGAAGAGGCGCTGCG
  CCGTGAGGCCGGGCTGCTGGAGGAGGCAGTGGCGGGCTCCGGAGCCCCGGGAGAGGTGGACAGCGCCGGC
  GCTGAGGTGACCAGCGCGCTTCTCAGCCGGGTGACCGCCTCGGCCCCTGGCCCTGCGGCCCCCGACCCTC
  CGGGCACTGGCGCTTCGGGGGCCACGGTCGTCTCAGGTTCAGCCTCAGGTCCTGCGGCTCCGGGTAAAGT
  TGGAAGTGTTGCTGTGGAAGACCAGCCAGATGTCAGTGCCGTGTTGTCAGCCTACAACCAACAAGGAGAT
  CCCACAATGTATGAAGAATACTATAGTGGACTGAAACACTTCATTGAATGTTCCCTGGACTGCCATCGGG
  CAGAGTTGTCCCAACTTTTTTATCCTCTGTTTGTGCACATGTACTTGGAGCTAGTCTACAATCAACATGA
  GAATGAAGCAAAGTCATTCTTTGAGAAGTTCCATGGAGATCAGGAATGTTATTACCAGGATGACCTACGA
  GTATTATCTAGTCTTACCAAAAAGGAACACATGAAAGGGAATGAGACCATGTTGGATTTTCGAACAAGTA
  AATTTGTTCTGCGTATTTCCCGTGACTCGTACCAACTCTTGAAGAGGCATCTTCAGGAGAAACAGAACAA
  TCAGATATGGAACATAGTTCAGGAGCACCTCTACATTGACATCTTTGATGGGATGCCGCGTAGTAAGCAA
  CAGATAGATGCGATGGTGGGAAGTTTGGCAGGAGAGGCTAAACGAGAGGCAAACAAATCAAAGGTATTTT
  TTGGTTTATTAAAAGAACCAGAAATTGAGGTACCTTTGGATGACGAGGATGAAGAGGGAGAAAATGAAGA
  AGGAAAACCTAAAAAGAAGAAGCCTAAAAAAGATAGTATTGGATCCAAAAGCAAAAAACAAGATCCCAAT
  GCTCCACCTCAGAACAGAATCCCTCTTCCTGAGTTGAAAGATTCAGATAAGTTGGATAAGATAATGAATA
  TGAAAGAAACCACCAAACGAGTGCGCCTTGGGCCGGACTGCTTACCCTCCATTTGTTTCTATACATTTCT
  CAATGCTTACCAGGGTCTCACTGCAGTGGATGTCACTGATGATTCTAGTCTGATTGCTGGAGGTTTTGCA
  GATTCAACTGTCAGAGTGTGGTCGGTAACACCCAAAAAGCTTCGTAGTGTCAAACAAGCATCAGATCTTA
  GTCTTATAGACAAAGAATCAGATGATGTCTTAGAAAGAATCATGGATGAGAAAACAGCAAGTGAGTTGAA
  GATTTTGTATGGTCACAGTGGGCCTGTCTACGGAGCCAGCTTCAGTCCGGATAGGAACTATCTGCTTTCC
  TCTTCAGAGGACGGAACTGTTAGATTGTGGAGCCTTCAAACATTTACTTGTTTGGTGGGATATAAAGGAC
  ACAACTATCCAGTATGGGACACACAATTTTCTCCATATGGATATTATTTTGTGTCAGGGGGCCATGACCG
  AGTAGCTCGGCTCTGGGCTACAGACCACTATCAGCCTTTAAGAATATTTGCCGGCCATCTTGCTGATGTG
  AATTGTACCAGATTCCATCCAAATTCTAATTATGTTGCTACGGGCTCTGCAGACAGAACTGTGCGGCTCT
  GGGACGTCCTGAATGGTAACTGTGTAAGGATCTTCACTGGACACAAGGGACCAATTCATTCCTTGACATT
  TTCTCCCAATGGGAGATTCCTGGCTACAGGAGCAACAGATGGCAGAGTGCTTCTTTGGGATATTGGACAT
  GGTTTGATGGTTGGAGAATTAAAAGGCCACACTGATACAGTCTGTTCACTTAGGTTTAGTAGAGATGGTG
  AAATTTTGGCATCAGGTTCAATGGATAATACAGTTCGATTATGGGATGCTATCAAAGCCTTTGAAGATTT
  AGAGACCGATGACTTTACTACAGCCACTGGGCATATAAATTTACCTGAGAATTCACAGGAGTTATTGTTG
  GGAACATATATGACCAAATCAACACCAGTTGTACACCTTCATTTTACTCGAAGAAACCTGGTTCTAGCTG
  CAGGAGCTTATAGTCCACAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006951
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006951.3, NP_008882.2
• RefSeq Size: 3283 bp
• RefSeq ORF: 2403 bp
• Locus ID: 6877
• Cytogenetics: 10q24.33
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).