Ficolin 2 (FCN2) (NM_015837) Human Untagged Clone

CAT#: SC304354

FCN2 (untagged)-Human ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) (FCN2), transcript variant SV1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FCN2
• Synonyms: EBP-37; FCNL; ficolin-2; P35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015837, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCTGGACAGAGCTGTGGGGGTCCTGGGCGCTGCCACCCTGCTGCTCTCTTTCCTGGGCATGGCCT
  GGGCTCTCCAGGCGGCAGACACCTGTCCAGGAGAACGTGGCCCCCCTGGACCTCCTGGGAAGGCAGGACC
  ACCTGGGCCCAACGGAGCACCTGGGGAGCCCCAGCCGTGCCTGACAGGCCCGCGTACCTGCAAGGACCTG
  CTAGACCGAGGGCACTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGC
  TCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGGTGGATGGCTCTGTGGACTT
  CTACCGGGACTGGGCCACGTACAAGCAGGGCTTCGGCAGTCGGCTGGGGGAGTTCTGGCTGGGGAATGAC
  AACATCCACGCCCTGACCGCCCAGGGAACCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGACAACT
  ACCAGTTTGCTAAGTACAGATCATTCAAGGTGGCCGACGAGGCGGAGAAGTACAATCTGGTCCTGGGGGC
  CTTCGTGGAGGGCAGTGCGGGAGATTCCCTGACGTTCCACAACAACCAGTCCTTCTCCACCAAAGACCAG
  GACAATGATCTTAACACCGGAAATTGTGCTGTGATGTTTCAGGGAGCTTGGTGGTACAAAAACTGCCATG
  TGTCAAACCTGAATGGTCGCTACCTCAGGGGGACTCATGGCAGCTTTGCAAATGGCATCAACTGGAAGTC
  GGGGAAAGGATACAATTATAGCTACAAGGTGTCAGAGATGAAGGTGCGACCTGCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015837
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015837.2, NP_056652.1
• RefSeq Size: 943 bp
• RefSeq ORF: 828 bp
• Locus ID: 2220
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (SV1) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a.