DENND1A (NM_020946) Human Untagged Clone

CAT#: SC304873

DENND1A (untagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DENND1A
• Synonyms: FAM31A; KIAA1608
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304873 representing NM_020946
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCAGGATCAAGCAGAATCCAGAGACCACATTTGAAGTATATGTTGAAGTGGCCTATCCCAGGA
  CAGGTGGCACTCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGACTACAGTGACCAGGAAGTTCT
  ACAGACTTTGACCAAGTTTTGTTTCCCCTTCTATGTGGACAGCCTCACAGTTAGCCAAGTTGGCCAGAAC
  TTCACATTCGTGCTCACTGACATTGACAGCAAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAGCGA
  AGAGCTGCTTCTGTATCTTAAGCTATCTCCCCTGGTTCGAGGTATTTTATAAGCTGCTTAACATCCTGGC
  AGATTACACGACAAAAAGACAGGAAAATCAGTGGAATGAGCTTCTTGAAACTCTGCACAAACTTCCCATC
  CCTGACCCAGGAGTGTCTGTCCATCTCAGCGTGCATTCTTATTTTACTGTGCCTGATACCAGAGAACTTC
  CCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGATGTTAACAACATGTTGCATCT
  GTACGCCAGTATGCTGTACGAACGCCGGATACTCATCATTTGCAGCAAACTCAGCACTCTGACTGCCTGC
  ATCCACGGGTCTGCGGCGATGCTCTACCCCATGTACTGGCAGCACGTGTACATCCCCGTGCTGCCGCCGC
  ATCTGCTGGACTACTGCTGTGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAAAGT
  CAGAAACATGGCCCTGGATGATGTCGTGATCCTGAATGTGGACACCAACACCCTGGAAACCCCCTTCGAT
  GACCTCCAGAGCCTCCCAAACGACGTGATCTCTTCCCTGAAGAACAGGCTGAAAAAGGTCTCCACAACCA
  CTGGGGATGGTGTGGCCAGAGCGTTCCTCAAGGCCCAGGCTGCTTTCTTCGGTAGCTACCGAAACGCTCT
  GAAAATCGAGCCGGAGGAGCCGATCACTTTCTGTGAGGAAGCCTTCGTGTCCCACTACCGCTCCGGAGCC
  ATGAGGCAGTTCCTGCAGAACGCCACACAGCTGCAGCTCTTCAAGCAGTTTATTGATGGTCGATTAGATC
  TTCTCAATTCCGGCGAAGGTTTCAGTGATGTTTTTGAAGAGGAAATCAACATGGGCGAGTACGCTGGCAG
  TGACAAACTGTACCATCAGTGGCTCTCCACTGTCCGGAAAGGAAGTGGAGCAATTCTGAATACTGTAAAG
  ACCAAAGCAAATCCGGCCATGAAGACTGTCTACAAGTTCGCAAAAGATCATGCAAAAATGGGAATAAAAG
  AGGTGAAAAACCGCTTGAAGCAAAAGGACATTGCCGAGAATGGCTGCGCCCCCACCCCAGAAGAGCAGCT
  GCCAAAGACTGCACCGTCCCCACTGGTGGAGGCCAAGGACCCCAAGCTCCGAGAAGACCGGCGGCCAATC
  ACAGTCCACTTTGGACAGGTGCGCCCACCTCGTCCACATGTTGTTAAGAGACCAAAGAGCAACATCGCAG
  TGGAAGGCCGGAGGACGTCTGTGCCGAGCCCTGAGCAGCCGCAGCCGTATCGGACACTCAGGGAGTCAGA
  CAGCGCGGAAGGCGACGAGGCAGAGAGTCCAGAGCAGCAAGTGCGGAAGTCCACAGGCCCTGTCCCAGCT
  CCCCCTGACCGGGCTGCCAGCATCGACCTTCTGGAAGACGTCTTCAGCAACCTGGACATGGAGGCCGCAC
  TGCAGCCACTGGGCCAGGCCAAGAGCTTAGAGGACCTTCGTGCCCCCAAAGACCTGAGGGAGCAGCCAGG
  GACCTTTGACTATCAGAGGCTGGATCTGGGCGGGAGTGAGAGGAGCCGCGGGGTGACAGTGGCCTTGAAG
  CTTACCCACCCGTACAACAAGCTCTGGAGCCTGGGCCAGGACGACATGGCCATCCCCAGCAAGCCCCCAG
  CTGCCTCCCCTGAGAAGCCCTCGGCCCTGCTCGGGAACTCCCTGGCCCTGCCTCGAAGGCCCCAGAACCG
  GGACAGCATCCTGAACCCCAGTGACAAGGAGGAGGTGCCCACCCCTACTCTGGGCAGCATCACCATCCCC
  CGGCCCCAAGGCAGGAAGACCCCAGAGCTGGGCATCGTGCCTCCACCGCCCATTCCCCGCCCGGCCAAGC
  TCCAGGCTGCCGGCGCCGCACTTGGTGACGTCTCAGAGCGGCTGCAGACGGATCGGGACAGGCGAGCTGC
  CCTGAGTCCAGGGCTCCTGCCTGGTGTTGTCCCCCAAGGCCCCACTGAACTGCTCCAGCCGCTCAGCCCT
  GGCCCCGGGGCTGCAGGCACGAGCAGTGACGCCCTGCTCGCCCTCCTGGACCCGCTCAGCACAGCCTGGT
  CAGGCAGCACCCTCCCGTCACGCCCCGCCACCCCGAATGTAGCCACCCCATTCACCCCCCAATTCAGCTT
  CCCCCCTGCAGGGACACCCACCCCATTCCCACAGCCACCACTCAACCCCTTTGTCCCATCCATGCCAGCA
  GCCCCACCCACCCTGCCCCTGGTCTCCACACCAGCCGGGCCTTTCGGGGCCCCTCCAGCTTCCCTGGGGC
  CGGCTTTTGCGTCCGGCCTCCTGCTGTCCAGTGCTGGCTTCTGTGCCCCTCACAGGTCTCAGCCCAACCT
  CTCCGCCCTCTCCATGCCCAACCTCTTTGGCCAGATGCCCATGGGCACCCACACGAGCCCCCTACAGCCG
  CTGGGTCCCCCAGCAGTTGCCCCGTCGAGGATCCGAACGTTGCCCCTGGCCCGCTCAAGTGCCAGGGCTG
  CTGAGACCAAGCAGGGGCTGGCCCTGAGGCCTGGAGACCCCCCGCTTCTGCCTCCCAGGCCCCCTCAAGG
  CCTGGAGCCAACACTGCAGCCCTCTGCTCCTCAACAGGCCAGAGACCCCTTTGAGGATTTGTTACAGAAA
  ACCAAGCAAGACGTGAGCCCGAGTCCGGCCCTGGCCCCGGCCCCAGACTCGGTGGAGCAGCTCAGGAAGC
  AGTGGGAGACCTTCGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: Please inquire
• ACCN: NM_020946
• ORF Size: 3030 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_020946.1, NP_065997.1
• RefSeq Size: 5041
• RefSeq ORF: 3030
• Locus ID: 57706
• Gene Summary: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]). [supplied by OMIM, Nov 2010]
  Transcript Variant: This variant (1) encodes isoform 1.