KIF25 (NM_030615) Human Untagged Clone

CAT#: SC305269

KIF25 (untagged)-Human kinesin family member 25 (KIF25), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIF25
• Synonyms: KNSL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030615, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACATGGACCTCAGGTCAGCTTCAGCGTGAGAAGCAGGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCT
  TCCCAGATGACAAGGACCTCAGGGTTTATGGTCCAGCAGAGTCTCAGAGCGCGGTCTTTGGAGATGTGTG
  CCCCCTACTCACTTCTCTCTTGGATGGGTACAATGTTTGTGTTATGGCGTATGGACAGACGGGCAGCGGA
  AAGAGCTATACCATGCTGGGACGCCATTCGGACGACGGCCCTGTTCTGCCGCTTGACCCACAGAGTGACT
  TAGGAATTATCCCTAGAGTGGCTGAGGAGCTCTTCAGGCTCATTTTGGAAAATACCTCAAGAAGCCCAAA
  GGTTGAAGTCTCCATAGTGGAAGTTTACAATAATGACATTTTTGACCTTCTGGCCAAAGACAGCATTGCA
  GCAGTGTCGGGGGTCAAGCGTGAGGTGGTGACAGCCAAGGATGGACGGACAGAGGTTGCGCTGCTGGCCT
  CTGAGGCTGTCGGCAGCGCCTCGAAACTGATGGAGCTCGTTCATGGAGGTCTGCAGCTCAGGGCGAAGCA
  CCCCACCCTGGTGCACGCGGATTCCTCCAGGTCTCACCTGATAATTACGGTGACTCTAACCACAGCCTCC
  TGCTCTGACAGCACTGCAGACCAAGCCTGCAGTGCCACCCTCCCCAGGGAGCAAACAGAGGCAGGAAGGG
  CAGGAAGGAGCCGCAGAGCTTCTCAAGGGGCCTTGGCTCCACAGCTGGTTCCTGGGAACCCCGCAGGGCA
  TGCGGAGCAGGTGCAGGCTCGACTACAGCTCGTGGACTCGGCCGGCAGCGAGTGCGTTGGTGTGTCTGGA
  GTGACCGGGTTGGCCCTGAGGGAGATGGCGTGCATCAGCCGCAGCCTTGCGGCCCTGGCAGGCGTCCTGG
  GGGCTTTGTTGGAGCACCGTGGCCATGCCCCGTACCGGAACAGCAGGCTCACCCACCTCCTTCAGGACTG
  CCTCGGAGGCGATGCGAAGTTACTGGTGATTCTCTGCATTTCTCCCAGCCAGAGGCACCTGGCACAGACG
  TTGCAGGGCCTGGGTTTCGGGATCCGAGCTCGGCAAGTCCAGCGAGGCCCTGCCCGAAAGAAGCCGCCCA
  GCTCCCAAACGGAGGGGAAGAGGAGGCCGGATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_030615
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030615.2, NP_085118.2
• RefSeq Size: 1510 bp
• RefSeq ORF: 1155 bp
• Locus ID: 3834
• Cytogenetics: 6q27
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This transcript (1) contains exon 8, and encodes the full length isoform.