EYA1 (NM_172058) Human Untagged Clone

CAT#: SC306709

EYA1 (untagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA1
• Synonyms: BOP; BOR; BOS1; OFC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172058, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAATGCAGGATCTAACCAGCCCGCATAGCCGTCTGAGTGGTAGTAGTGAATCCCCCAGTGGCCCCA
  AACTCGGTAACTCTCATATAAATAGTAATTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAAT
  GAGCAGCAGTGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGG
  AGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCAT
  ACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGG
  AATGCAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGT
  GCATTGTGGGCAGGCATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCTGGACAGACAGGATTTC
  TCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCACCATACAGCTACCAGATGCAAGGTAG
  CAGTTTTACAACATCATCAGGAATATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGT
  TCACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCAC
  CGTATCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCA
  GCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGCACAATC
  CACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATCAC
  GTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTG
  GGACTTGGATGAGACAATCATTGTTTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGG
  GATCCACCCACTTCAGTTTCCCTTGGACTGCGAATGGAAGAAATGATTTTCAACTTGGCAGACACACATT
  TATTTTTTAATGACTTAGAAGAATGTGACCAAGTCCATATAGATGATGTTTCTTCAGATGATAACGGACA
  GGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAGTGCTAACTTATGTTTG
  GCAACTGGTGTACGGGGCGGTGTGGACTGGATGAGAAAGTTGGCCTTCCGCTACAGACGGGTAAAAGAGA
  TCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTT
  GAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGTTGACACTGGCCCTGAAAGCACTCTCGCTCATTCAC
  TCCCGGACAAACTGTGTGAATATTTTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGC
  TGTATGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTG
  TTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGGAGATGGTGTAGAAGAA
  GAACAAGGAGCAAAAAAGCACGCGATGCCCTTCTGGAGGATCTCCAGCCACTCGGACCTCATGGCCCTGC
  ACCATGCCTTGGAACTGGAGTACCTGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_172058
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172058.3, NP_742055.1
• RefSeq Size: 3933 bp
• RefSeq ORF: 1779 bp
• Locus ID: 2138
• Cytogenetics: 8q13.3
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (EYA1B) differs in the 5' UTR compared to variant EYA1C. Variants EYA1B and EYA1C encode the same isoform (1).