G CSF (CSF3) (NM_172220) Human Untagged Clone

CAT#: SC306745

CSF3 (untagged)-Human colony stimulating factor 3 (granulocyte) (CSF3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSF3
• Synonyms: C17orf33; CSF3OS; GCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172220 edited
  AATGGGGATTAAAGGCACCCAGTGTCCCCGAGAGGGCCTCAGGTGGTAGGGAACAGCATG
  TCTCCTGAGCCCGCTCTGTCCCCAGCCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGG
  ACAGTGCAGGAAGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTC
  AAGTGCTTAGAGCAAGTGAGGAAGATCCAGGGCGATGGCGCAGCGCTCCAGGAGAAGCTG
  TGTGCCACCTACAAGCTGTGCCACCCCGAGGAGCTGGTGCTGCTCGGACACTCTCTGGGC
  ATCCCCTGGGCTCCCCTGAGCAGCTGCCCCAGCCAGGCCCTGCAGCTGGCAGGCTGCTTG
  AGCCAACTCCATAGCGGCCTTTTCCTCTACCAGGGGCTCCTGCAGGCCCTGGAAGGGATC
  TCCCCCGAGTTGGGTCCCACCTTGGACACACTGCAGCTGGACGTCGCCGACTTTGCCACC
  ACCATCTGGCAGCAGATGGAAGAACTGGGAATGGCCCCTGCCCTGCAGCCCACCCAGGGT
  GCCATGCCGGCCTTCGCCTCTGCTTTCCAGCGCCGGGCAGGAGGGGTCCTGGTTGCCTCC
  CATCTGCAGAGCTTCCTGGAGGTGTCGTACCGCGTTCTACGCCACCTTGCCCAGCCCTGA
  GCCAAGCCCTCCCCATCCCATGTATTTATCTCTATTTAATATTTATGTCTATTTAAGCCT
  CATATTTAAAGACAGGGAAGAGCAGAACGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172220 unedited
  GTCAGTCAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATCGCCCTTAAGGGGATTA
  AAGGCACCCAGTGTCCCCGAGNAGGCCTCAGGTGGTAGGGAACAGCATGTCTCCTGAGCC
  CGCTCTGTCCCCAGCCCTGCAGCTGCTGCTGTGGCACAGTGCACTCTGGACAGTGCAGGA
  AGCCACCCCCCTGGGCCCTGCCAGCTCCCTGCCCCAGAGCTTCCTGCTCAAGTGCTTAGA
  GCAAGTGAGGAAGATCCAGGGCGATGGCGCAGCGCTCCAGGAGAAGCTGTGTGCCACCTA
  CAAGCTGTGCCACCCCGAGGAGCTGGTGCTGCTCGGACACTCTCTGGGCATCCCCTGGGC
  TCCCCTGAGCAGCTGCCCCAGCCAGGCCCTGCAGCTGGCAGGCTGCTTGAGCCAACTCCA
  TAGCGGCCTTTTCCTCTACCAGGGGCTCCTGCAGGCCCTGGAAGGGATCTCCCCCGAGTT
  GGGTCCCACCTTGGACACACTGCAGCTGGACGTCGCCGACTTTGCCACCACCATCTGGCA
  GCAGATGGAAGAACTGGGAATGGCCCCTGCCCTGCAGCCCACCCAGGGTGCCATGCCGGC
  CTTCGCCTCTGCTTTCCAGCGCCGGGCNAGGAGGGTCCTGGTTGCCTCCCATCTGCAGAG
  CTTCCTGGAGGTGTCGTACCGCGTTCTACGCCACCTTGCCCAGCCCTGAGCCAAGCCCTC
  CCCATCCCATGTATTTATCTCTATTTAATATTATGTCTATTTAAGCCTCATATTTTAAGA
  CAGGNAAGAGCAGAACGGGAGGGCGAATTCAGATCTTGTACCGATATTCAGCTTGTCGAC
  TCTAGATTGCGGCCGCGTCATAGCTGGTTCCTGAACAGATCCCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_172220
• Insert Size: 750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172220.1, NP_757374.1
• RefSeq Size: 1703 bp
• RefSeq ORF: 603 bp
• Locus ID: 1440
• Cytogenetics: 17q21.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
• Gene Summary: 'This gene encodes a member of the IL-6 superfamily of cytokines. The encoded cytokine controls the production, differentiation, and function of granulocytes. Granulocytes are a type of white blood cell that are part of the innate immune response. A modified form of this protein is commonly administered to manage chemotherapy-induced neutropenia. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2020]'
  Transcript Variant: This variant (3) lacks an in-frame exon in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a.