ACCN1 (ASIC2) (NM_183377) Human Untagged Clone

CAT#: SC307569

ASIC2 (untagged)-Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASIC2
• Synonyms: ACCN; ACCN1; ASIC2a; BNaC1; BNC1; hBNaC1; MDEG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_183377, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCGGATTGGCGGAGCCGGGCTGCCCGCAGCCGCGCTCACCGGCCCGGGACGCTTCCGCATGGCCC
  GCGAGGAGCCGGCGCCCGCGGCGTTGGCGGCTGCCGGGCAGCCCGGGGGCGGCAGAGGCGGCGAGCGGGC
  GCTGCAGGGGCCAGGGGTCGCCCGCAGGGGGCGGCCATCGCTGAGCCGCGCTAAACTGCACGGGCTGCGG
  CACATGTGTGCCGGGCGCACGGCGGCTGGGGGCTCCTTCCAGCGGCGGGCGCTGTGGGTGCTGGCCTTCT
  GTACATCCTTCGGCTTGCTGCTGTCCTGGTCCTCGAACCGCTTGCTCTACTGGCTCAGCTTCCCGTCACA
  CACGCGGGTGCACCGCGAGTGGAGCCGCCAGTTACCCTTCCCCGCCGTCACTGTGTGCAACAACAACCCG
  CTGCGCTTCCCGCGCCTCTCCAAGGGGGACCTCTACTATGCCGGCCACTGGCTCGGGCTGCTGCTGCCCA
  ACCGCACCGCGCGCCCGCTTGTCAGCGAGCTGCTGCGGGGCGACGAGCCGCGCCGCCAGTGGTTCCGCAA
  GCTGGCGGACTTCCGCCTCTTCCTGCCTCCGCGCCACTTCGAGGGAATCAGCGCCGCCTTCATGGACCGC
  CTGGGCCACCAGCTGGAGGACATGCTGCTCTCCTGCAAGTACCGCGGCGAGCTCTGCGGGCCGCACAACT
  TCTCCTCCGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAACCTCTGCT
  CACCACGGTCAAGGGGGGGACAGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAGGATGAGTACCTG
  CCCATCTGGGGAGAGACAGAGGAAACGACATTTGAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTG
  AGCCACCTTTCATCCAAGAGCTGGGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGA
  GCAGAGGCTCACATACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTT
  CCTGTTTACAGCATCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGCAACTGCC
  GCATGGTTCACATGCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGC
  CCTAGGTCTGTTGGCGGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTAACCCGCTAC
  AACAAAGAGCTCTCCATGGTGAAGATCCCCAGCAAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACA
  AATCAGAAAAATATATCTCAGAGAACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGAC
  AATTGAACAGAAGAAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTC
  ATTGGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTAT
  TAGACCTGCTTGGCAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGTACTTGTGACACAATGCC
  AAACCACTCTGAAACCATCAGTCACACTGTGAACGTGCCCCTGCAGACGACCCTGGGGACCTTGGAGGAG
  ATTGCCTGCTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_183377
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: A TrueClone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183377.1, NP_899233.1
• RefSeq Size: 3470 bp
• RefSeq ORF: 1692 bp
• Locus ID: 40
• Cytogenetics: 17q11.2-q12
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Taste transduction
• Gene Summary: 'This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]'
  Transcript Variant: This variant (MDEG2) represents the longer transcript and encodes the longer isoform (MDEG2).