CACNB1 (NM_199247) Human Untagged Clone

CAT#: SC307910

CACNB1 (untagged)-Human calcium channel, voltage-dependent, beta 1 subunit (CACNB1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNB1
• Synonyms: CAB1; CACNLB1; CCHLB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_199247 edited
  GGCAGCCGAAGGCCGAGCTGGGTGGCTGGACCGGGTGCTGGCTGCGCCGCGCTGCTTTCG
  GCTCCCACGGCCTCTCCCATGCGCTGAGGGAGCCCGGCTGGGCCGGGCCGGCGGCGGGAG
  GGGAGGCTCCTCTCCATGGTCCAGAAGACCAGCATGTCCCGGGGCCCTTACCCACCCTCC
  CAGGAGATCCCCATGGAGGTCTTCGACCCCAGCCCGCAGGGCAAATACAGCAAGAGGAAA
  GGGCGATTCAAACGGTCAGATGGGAGCACGTCCTCGGATACCACATCCAACAGCTTTGTC
  CGCCAGGGCTCAGCGGAGTCCTACACCAGCCGTCCATCAGACTCTGATGTATCTCTGGAG
  GAGGACCGGGAAGCCTTAAGGAAGGAAGCAGAGCGCCAGGCATTAGCGCAGCTCGAGAAG
  GCCAAGACCAAGCCAGTGGCATTTGCTGTGCGGACAAATGTTGGCTACAATCCGTCTCCA
  GGGGATGAGGTGCCTGTGCAGGGAGTGGCCATCACCTTCGAGCCCAAAGACTTCCTGCAC
  ATCAAGGAGAAATACAATAATGACTGGTGGATCGGGCGGCTGGTGAAGGAGGGCTGTGAG
  GTTGGCTTCATTCCCAGCCCCGTCAAACTGGACAGCCTTCGCCTGCTGCAGGAACAGAAG
  CTGCGCCAGAACCGCCTCGGCTCCAGCAAATCAGGCGATAACTCCAGTTCCAGTCTGGGA
  GATGTGGTGACTGGCACCCGCCGCCCCACACCCCCTGCCAGTGGTAATGAAATGACTAAC
  TTAGCCTTTGAACTAGACCCCCTAGAGTTAGAGGAGGAAGAGGCTGAGCTTGGTGAGCAG
  AGTGGCTCTGCCAAGACTAGTGTTAGCAGTGTCACCACCCCGCCACCCCATGGCAAACGC
  ATCCCCTTCTTTAAGAAGACAGAGCATGTGCCCCCCTATGACGTGGTGCCTTCCATGAGG
  CCCATCATCCTGGTGGGACCGTCGCTCAAGGGCTACGAGGTTACAGACATGATGCAGAAA
  GCTTTATTTGACTTCTTGAAGCATCGGTTTGATGGCAGGATCTCCATCACTCGTGTGACG
  GCAGATATTTCCCTGGCTAAGCGCTCAGTTCTCAACAACCCCAGCAAACACATCATCATT
  GAGCGCTCCAACACACGCTCCAGCCTGGCTGAGGTGCAGAGTGAAATCGAGCGAATCTTC
  GAGCTGGCCCGGACCCTTCAGTTGGTCGCTCTGGATGCTGACACCATCAATCACCCAGCC
  CAGCTGTCCAAGACCTCGCTGGCCCCCATCATTGTTTACATCAAGATCACCTCTCCCAAG
  GTACTTCAAAGGCTCATCAAGTCCCGAGGAAAGTCTCAGTCCAAACACCTCAATGTCCAA
  ATAGCGGCCTCGGAAAAGCTGGCACAGTGCCCCCCTGAAATGTTTGACATCATCCTGGAT
  GAGAACCAATTGGAGGATGCCTGCGAGCATCTGGCGGAGTACTTGGAAGCCTATTGGAAG
  GCCACACACCCGCCCAGCAGCACGCCACCCAATCCGCTGCTGAACCGCACCATGGCTACC
  GCAGCCCTGGCTGCCAGCCCTGCCCCTGTCTCCAACCTCCAGGTACAGGTGCTCACCTCG
  CTCAGGAGAAACCTCGGCTTCTGGGGCGGGCTGGAGTCCTCACAGCGGGGCAGTGTGGTG
  CCCCAGGAGCAGGAACATGCCATGTAGTGGGCGCCCTGCCCGTCTTCCCTCCTGCTCTGG
  GGTCGGAACTGGAGTGCAGGGAACATGGAGGAGGAAGGGAAGAGCTTTATTTTGTAAAAA
  AATAAGATGAGCGGCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199247 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCAGCCGAAGGCCGA
  GCTGGGTGGCTGGACCGGGTGCTGGCTGCGCCGCGCTGCTTTCGGCTCCCACGGCCTCTC
  CCATGCGCTGAGGGAGCCCGGCTGGGCCGGGCCGGCGGCGGGAGGGGAGGCTCCTCTCCA
  TGGTCCAGAAGACCAGCATGTCCCGGGGCCCTTACCCACCCTCCCAGGAGATCCCCATGG
  AGGTCTTCGACCCCAGCCCGCAGGGCAAATACAGCAAGAGGAAAGGGCGATTCAAACGGT
  CAGATGGGAGCACGTCCTCGGATACCACATCCAACAGCTTTGTCCGCCAGGGCTCAGCGG
  AGTCCTACACCAGCCGTCCATCAGACTCTGATGTATCTCTGGAGGAGGACCGGGAAGCCT
  TAAGGAAGGAAGCAGAGCGCCAGGCATTAGCGCAGCTCGAGAAGGCCAAGACCAAGCCAG
  TGGCATTTGCTGTGCGGACAAATGTTGGCTACAATCCGTCTCCAGGGGATGAGGTGCCTG
  TGCAGGGAGTGGCCATCACCTTCGAGCCCAAAGACTTCCTGCACATCAAGGAGAAATACA
  ATAATGACTGGTGGATCGGGCGGCTGGTGAAGGAGGGCTGTGAGGTTGGCTTCATTCCCA
  GCCCCGTCAAACTGGACAGCCTTCGCCTGCTGCAGGAACAGAAGCTGCGCCAGAACCGCC
  TCGGCTCCAGCANATCAGGGCGATACTCCAGNTCCAGTCTGGGAGATGTGGTGACTGGCA
  CCCGCCGCCCCACACCCCCTGCCAGTGGTAATGAAATGACTAACTTAGCCTNTGAACTAN
  ACCCCCTANATTAGAAGAGGAAGAGGCTGACCTGGTGAGCANAGTGGCTCTGCCAGACTA
  TGTTAGCAGTGGACCACCCCGCACCCATGGNAACGCATCCCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_199247 unedited
  GGCCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCCGCTCATCTTATTTTTTTAC
  AAAATAAAGCTCTTCCCTTCCTCCTCCATGTTCCCTGCACTCCAGTTCCGACCCCAGAGC
  AGGAGGGAAGACGGGCAGGGCGCCCACTACATGGCATGTTCCTGCTCCTGGGGCACCACA
  CTGCCCCGCTGTGAGGACTCCAGCCCGCCCCAGAAGCCGAGGTTTCTCCTGAGCGAGGTG
  AGCACCTGTACCTGGAGGTTGGAGACAGGGGCAGGGCTGGCAGCCAGGGCTGCGGTAGCC
  ATGGTGCGGTTCAACAGCGGATTGGGTGGCGTGCTGCTGGGCGGGTGTGTGGCCTTCCAA
  TAGGCTTCCAAGTACTCCGCCAGATGCTCGCAGGCATCCTCCAATTGGTTCTCATCCAGG
  ATGATGTCAAACATTTCAGGGGGGCACTGTGCCAGCTTTTCCGAGGCCGCTATTTGGACA
  TTGAGGTGTTTGGACTGAGACTTTCCTCGGGACTTGATGAGCCTTTGAAGTACCTTGGGA
  GAGGTGATCTTGATGTAAACAATGATGGGGGCCAGCGAGGTCTTGGACAGCTGGGCTGGG
  TGATTGATGGTGTCAGCATCCAGAGCGACCAACTGAAGGGTCCGGGCCAGCTCGAAGATT
  CGCTCGATTTCACTCTGCACCCTCACCAGGCTGGAGCGTGTGTTGGAGCGCTCAATGATG
  ATGTGTTTGCTGGGGGTTGTTGAAAACTGAGCGCTTATCCAAGGAAATATCTGCCGCCCA
  ACGAGTGATGGAGATCCCGCCATCAACCGATCCTTCAAGAGTCAAAAAAGCTTCTGCCTC
  AGTCTGGAACCTCGACCCTTGACCCACGCCCACCCAGAGATGGGGCCCATGGAAGGACCA
  CGTATAAGGGGGCCATGCCTCTGCCCTTAAAAAAGGGAACCCTTCCCTGGGGGGCGCGGG
  GGGACCCGTCACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199247
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199247.1, NP_954855.1
• RefSeq Size: 1847 bp
• RefSeq ORF: 1572 bp
• Locus ID: 782
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the calcium channel beta subunit family. It plays an important role in the calcium channel by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2), also known as beta-1M, betaC, and beta-1a, differs in the 3' UTR and has multiple coding region differences, compared to variant 1. It encodes isoform 2 which has a distinct C-terminus, compared to isoform 1.