CtIP (RBBP8) (NM_203292) Human Untagged Clone

CAT#: SC308103

RBBP8 (untagged)-Human retinoblastoma binding protein 8 (RBBP8), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBBP8
• Synonyms: COM1; CTIP; JWDS; RIM; SAE2; SCKL2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_203292, the custom clone sequence may differ by one or more nucleotides
  ATGAACATCTCGGGAAGCAGCTGTGGAAGCCCTAACTCTGCAGATACATCTAGTGACTTT
  AAGGACCTTTGGACAAAACTAAAAGAATGTCATGATAGAGAAGTACAAGGTTTACAAGTA
  AAAGTAACCAAGCTAAAACAGGAACGAATCTTAGATGCACAAAGACTAGAAGAATTCTTC
  ACCAAAAATCAACAGCTGAGGGAACAGCAGAAAGTCCTTCATGAAACCATTAAAGTTTTA
  GAAGATCGGTTAAGAGCAGGCTTATGTGATCGCTGTGCAGTAACTGAAGAACATATGCGG
  AAAAAACAGCAAGAGTTTGAAAATATCCGGCAGCAGAATCTTAAACTTATTACAGAACTT
  ATGAATGAAAGGAATACTCTACAGGAAGAAAATAAAAAGCTTTCTGAACAACTCCAGCAG
  AAAATTGAGAATGATCAACAGCATCAAGCAGCTGAGCTTGAATGTGAGGAAGACGTTATT
  CCAGATTCACCGATAACAGCCTTCTCATTTTCTGGCGTTAACCGGCTACGAAGAAAGGAG
  AACCCCCATGTCCGATACATAGAACAAACACATACTAAATTGGAGCACTCTGTGTGTGCA
  AATGAAATGAGAAAAGTTTCCAAGTCTTCAACTCATCCACAACATAATCCTAATGAAAAT
  GAAATTCTAGTAGCTGACACTTATGACCAAAGTCAATCTCCAATGGCCAAAGCACATGGA
  ACAAGCAGCTATACCCCTGATAAGTCATCTTTTAATTTAGCTACAGTTGTTGCTGAAACA
  CTTGGACTTGGTGTTCAAGAAGAATCTGAAACTCAAGGTCCCATGAGCCCCCTTGGTGAT
  GAGCTCTACCACTGTCTGGAAGGAAATCACAAGAAACAGCCTTTTGAGGAATCTACAAGA
  AATACTGAAGATAGTTTAAGATTTTCAGATTCTACTTCAAAGACTCCTCCTCAAGAAGAA
  TTACCTACTCGAGTGTCATCTCCTGTATTTGGAGCTACCTCTAGTATCAAAAGTGGTTTA
  GATTTGAATACAAGTTTGTCCCCTTCTCTTTTACAGCCTGGGAAAAAAAAACATCTGAAA
  ACACTCCCTTTTAGCAACACTTGTATATCTAGATTAGAAAAAACTAGATCAAAATCTGAA
  GATAGTGCCCTTTTCACACATCACAGTCTTGGGTCTGAAGTGAACAAGATCATTATCCAG
  TCATCTAATAAACAGATACTTATAAATAAAAATATAAGTGAATCCCTAGGTGAACAGAAT
  AGGACTGAGTACGGTAAAGATTCTAACACTGATAAACATTTGGAGCCCCTGAAATCATTG
  GGAGGCCGAACATCCAAAAGGAAGAAAACTGAGGAAGAAAGTGAACATGAAGTAAGCTGC
  CCCCAAGCTTCTTTTGATAAAGAAAATGCTTTCCCTTTTCCAATGGATAATCAGTTTTCC
  ATGAATGGAGACTGTGTGATGGATAAACCTCTGGATCTGTCTGATCGATTTTCAGCTATT
  CAGCGTCAAGAGAAAAGCCAAGGAAGTGAGACTTCTAAAAACAAATTTAGGCAAGTGACT
  CTTTATGAGGCTTTGAAGACCATTCCAAAGGGCTTTTCCTCAAGCCGTAAGGCCTCAGAT
  GGCAACTGCACGTTGCCCAAAGATTCCCCAGGGGAGCCCTGTTCACAGGAATGCATCATC
  CTTCAGCCCTTGAATAAATGCTCTCCAGACAATAAACCATCATTACAAATAAAAGAAGAA
  AATGCTGTCTTTAAAATTCCTCTACGTCCACGTGAAAGTTTGGAGACTGAGAATGTTTTA
  GATGACATAAAGAGTGCTGGTTCTCATGAGCCAATAAAAATACAAACCAGGTCAGACCAT
  GGAGGATGTGAACTTGCATCAGTTCTTCAGTTAAATCCATGTAGAACTGGTAAAATAAAG
  TCTCTACAAAACAACCAAGATGTATCCTTTGAAAATATCCAGTGGAGTATAGATCCGGGA
  GCAGACCTTTCTCAGTATAAAATGGATGTTACTGTAATAGATACAAAGGATGGCAGTCAG
  TCAAAATTAGGAGGAGAGACAGTGGACATGGACTGTACATTGGTTAGTGAAACCGTTCTC
  TTAAAAATGAAGAAGCAAGAGCAGAAGGGAGAAAAAAGTTCAAATGAAGAAAGAAAAATG
  AATGATAGCTTGGAAGATATGTTTGATCGGACAACACATGAAGAGTATGAATCCTGTTTG
  GCAGACAGTTTCTCCCAAGCAGCAGATGAAGAGGAGGAATTGTCTACTGCCACAAAGAAA
  CTACACACTCATGGTGATAAACAAGACAAAGTCAAGCAGAAAGCGTTTGTGGAGCCGTAT
  TTTAAAGGTGATGAAAGTATTATGCAGATATGCCAGCAGAAGAAAGAGAAAAGAAATTGG
  CTTCCTGCTCAAGACACCGATTCCGCTACATTCCACCCAACACACCAGAGAATTTTTGGG
  AAGTTGGTTTTCCTTCCACTCAGACTTGTATGGAAAGAGGTTATATTAAGGAAGATCTTG
  ATCCTTGTCCTCGTCCAAAAAGACGTCAGCCTTACAACGCAATATTTTCTCCAAAAGGCA
  AGGAGCAGAAGACATAGACGTTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_203292
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203292.1, NP_976037.1
• RefSeq Size: 3233 bp
• RefSeq ORF: 2604 bp
• Locus ID: 5932
• Cytogenetics: 18q11.2
• Gene Summary: 'The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.