ASTN1 (NM_207108) Human Untagged Clone

CAT#: SC308385

ASTN1 (untagged)-Human astrotactin 1 (ASTN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASTN1
• Synonyms: ASTN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_207108, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTTTAGCCGGGCTCTGCGCCCTGCTCGCCTGCTGCTGGGGGCCGGCGGCGGTGCTGGCCACGGCCG
  CCGGCGACGTGGATCCATCCAAGGAGCTGGAGTGCAAGCTCAAAAGCATCACGGTGTCGGCACTGCCCTT
  CCTGCGCGAGAACGACCTGAGCATCATGCACAGCCCCTCGGCCTCGGAGCCCAAGCTCCTCTTCTCGGTG
  CGCAACGACTTCCCGGGAGAAATGGTCGTGGTGGACGACCTGGAGAACACGGAGCTGCCCTACTTCGTGC
  TGGAGATCTCAGGGAACACAGAGGATATCCCTTTGGTGCGCTGGAGGCAGCAGTGGCTGGAGAATGGCAC
  TTTGCTTTTTCACATTCATCACCAAGATGGTGCCCCAAGCCTTCCTGGACAAGACCCCACTGAAGAACCC
  CAACATGAGTCGGCAGAAGAGGAGCTGAGGATCCTCCACATCTCAGTCATGGGTGGCATGATCGCTCTGC
  TGCTGTCCATCTTGTGCCTGGTGATGATCCTGTATACTCGCCGGCGCTGGTGCAAACGCCGCCGGGTCCC
  GCAGCCCCAGAAGAGTGCCAGTGCTGAGGCAGCCAATGAGATTCACTACATTCCTTCTGTGCTGATCGGC
  GGGCACGGACGGGAGAGCCTGCGCAATGCCCGCGTGCAGGGCCACAACTCCAGTGGCACCCTGAGCATCC
  GGGAGACACCTATCCTGGACGGCTATGAGTATGACATCACTGATCTGCGCCACCATCTGCAGAGGGAGTG
  CATGAACGGAGGGGAGGACTTTGCCAGCCAGGTCACGCGCACCCTCGACTCCCTGCAGGGCTGCAATGAA
  AAGTCGGGGATGGACCTCACACCAGGAAGTGACAATGCCAAGCTGTCACTGATGAACAAGTATAAAGATA
  ATATTATAGCCACTAGCCCTGTGGACTCCAACCACCAGCAAGCCACCCTTCTCTCTCACACCTCCAGCAG
  CCAGAGAAAGCGGATCAACAACAAAGCAAGAGCTGGTTCCGCCTTCTTGAACCCTGAAGGGGATTCTGGC
  ACAGAGGCAGAAAACGACCCCCAGCTGACCTTTTACACGGATCCTTCAAGGAGCAGGAGGCGTAGTAGAG
  TGGGTTCTCCCCGAAGTCCTGTGAATAAGACCACCTTGACCCTGATCAGCATCACCAGCTGTGTGATTGG
  CCTCGTGTGCTCCTCTCACGTCAACTGCCCTCTCGTTGTCAAGATCACCCTGCATGTCCCTGAGCACCTG
  ATTGCTGATGGGAGCCGCTTCATCTTGCTGGAGGGGAGCCAGCTGGATGCCAGTGACTGGCTGAACCCTG
  CCCAAGTGGTTCTCTTCTCTCAGCAGAACTCCAGCGGACCCTGGGCCATGGACCTCTGTGCCCGGCGGCT
  CCTGGACCCCTGTGAACACCAATGTGACCCCGAAACTGGGGAATGCCTCTGCTATGAAGGCTACATGAAG
  GATCCAGTACATAAGCACCTTTGCATTCGGAACGAATGGGGGACAAACCAGGGGCCATGGCCTTACACAA
  TATTTCAGCGAGGCTTTGACCTGGTTTTGGGAGAGCAGCCCTCTGATAAAATATTTAGATTCACCTACAC
  TCTTGGGGAGGGCATGTGGTTGCCCCTCAGCAAGAGCTTTGTGATTCCACCAGCCGAACTGGCCATCAAT
  CCATCAGCAAAGTGCAAGACGGACATGACTGTGATGGAGGATGCTGTGGAGGTCAGAGAGGAGCTGATGA
  CTTCATCCTCCTTCGACAGCCTGGAGGTTCTCTTAGATTCCTTTGGGCCGGTGCGCGACTGCAGCAAAGA
  TAACGGGGGCTGCAGTAAGAATTTCCGCTGTATTTCAGATCGCAAGCTGGACTCCACTGGTTGCGTGTGC
  CCATCTGGACTCAGTCCCATGAAGGACAGCTCTGGCTGCTATGACCGCCACATCGGGGTGGACTGTTCCG
  ACGGCTTCAACGGCGGCTGTGAGCAGCTGTGCCTCCAGCAGATGGCGCCCTTCCCGGACGACCCCACCTT
  GTATAACATCCTCATGTTCTGTGGGTGCATCGAGGACTACAAGCTTGGTGTGGATGGACGCTCTTGCCAA
  CTCATCACGGAGACCTGTCCAGAGGGAAGTGACTGTGGGGAAAGCAGGGAGCTTCCCATGAACCAGACCC
  TCTTTGGGGAGATGTTCTTTGGTTACAACAACCATTCCAAGGAAGTGGCTGCCGGACAGGTGCTGAAAGG
  AACATTCAGGCAAAACAACTTTGCTCGTGGTTTAGACCAGCAACTGCCAGATGGTCTTGTGGTGGCCACT
  GTGCCCCTGGAGAATCAATGCCTAGAGGAGATCTCGGAGCCCACCCCTGACCCTGACTTCCTGACTGGGA
  TGGTGAACTTCAGTGAAGTGTCTGGGTACCCTGTGCTGCAGCACTGGAAGGTCCGGTCTGTGATGTACCA
  CATCAAACTCAACCAAGTGGCCATCTCTCAGGCCCTCAGCAATGCTCTCCACTCGCTGGATGGGGCTACA
  TCTCGTGCAGATTTTGTGGCGCTGTTGGACCAGTTCGGCAACCATTACATCCAGGAAGCTATCTACGGCT
  TTGAGGAGTCCTGTTCTATCTGGTACCCAAACAAGCAGGTCCAGCGGCGACTCTGGCTGGAGTATGAAGA
  CATCAGTAAAGGCAACTCCCCATCAGATGAGTCTGAGGAGCGGGAAAGAGACCCCAAGGTGCTGACATTC
  CCAGAATACATCACCAGCTTGTCAGACTCCGGCACCAAGCACATGGCGGCTGGAGTCCGCATGGAGTGCC
  ACAGCAAGGGACGATGCCCCTCGTCCTGCCCCCTGTGTCATGTGACATCCAGCCCTGACACCCCTGCTGA
  GCCGGTTCTGCTGGAGGTGACCAAAGCAGCCCCCATCTATGAACTAGTGACCAACAACCAGACCCAGAGG
  CTCTTGCAGGAGGCTACCATGAGCTCTCTCTGGTGCTCAGGGACTGGAGATGTCATCGAGGACTGGTGTC
  GATGTGACTCCACTGCTTTTGGAGCTGATGGACTCCCCACCTGTGCGCCTCTCCCACAGCCTGTGCTGAG
  ACTCTCCACGGTTCACGAGCCCAGCAGCACTCTTGTGGTCCTGGAGTGGGAACACTCAGAGCCACCAATC
  GGGGTGCAGATTGTAGATTACCTCCTCCGTCAAGAGAAAGTCACTGACAGGATGGACCACTCCAAAGTGG
  AGACAGAAACAGTGCTGAGCTTTGTGGACGACATCATCTCTGGAGCAAAGTCTCCTTGTGCAATGCCATC
  TCAGGTGCCGGACAAGCAGCTCACCACCATCTCTCTGATCATACGATGCCTGGAACCTGACACCATTTAC
  ATGTTCACGCTGTGGGGAGTGGACAACACAGGACGGCGCTCCAGGCCAAGCGACGTGATCGTGAAGACCC
  CATGCCCCGTGGTGGATGATGTCAAGGCTCAAGAAATAGCAGACAAGATCTACAATCTCTTCAATGGCTA
  CACTAGTGGGAAGGAGCAGCAGACCGCCTACAACACCCTCCTGGATCTGGGTTCCCCCACCTTACACCGG
  GTCCTCTACCACTATAACCAGCACTATGAGAGTTTTGGGGAATTCACCTGGCGATGTGAGGATGAGTTAG
  GTCCCAGGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_207108
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207108.2, NP_996991.1
• RefSeq Size: 3974 bp
• RefSeq ORF: 3651 bp
• Locus ID: 460
• Cytogenetics: 1q25.2
• Protein Families: Transmembrane
• Gene Summary: 'Astrotactin is a neuronal adhesion molecule required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb (Fink et al., 1995).[supplied by OMIM, Jun 2009]'
  Transcript Variant: This variant (2) uses a different splice site in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.