CLCN6 (NM_021736) Human Untagged Clone

CAT#: SC308816

CLCN6 (untagged)-Human chloride channel 6 (CLCN6), transcript variant ClC-6c

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN6
• Synonyms: CLC-6; KIAA0046
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021736, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCC
  GCACCCCCGAGGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGA
  CTATGAGAGTTTGGATTATGATCGCTGTATCAATGACCCTTACCTGGAAGTTTTGGAGACCATGGATAAT
  AAGAAAGGTCGAAGATATGAGGCGGTGAAGTGGATGGTGGTGTTTGCCATTGGAGTCTGCACTGGCCTGG
  TGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCAACTCAAGTTCGGAGTGGTACAGACATCGGT
  GGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCCTGGGTTTTAACCTCACCTTT
  GTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCCGAGGTCAAAT
  GCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGT
  GCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTG
  GGAGCTGGCCTCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTATTTCCGAA
  GCGACAGAGACAAGAGAGACTTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCC
  AATCGGGGGTACCTTGTTCAGTCTAGAGGAGGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTG
  CTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCTTCCGTTCTGGGATTCAGTTTGGAAGCTGGG
  GTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGAGTCTTAGAGAGCCTCCTTGTGTCTC
  TGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGTTAGGAGAATGCCGACAGATGTCCTCTTCGAG
  TCAAATCGGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_021736
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021736.2, NP_068504.1
• RefSeq Size: 1175 bp
• RefSeq ORF: 1062 bp
• Locus ID: 1185
• Cytogenetics: 1p36.22
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: 'This gene encodes a member of the voltage-dependent chloride channel protein family. Members of this family can function as either chloride channels or antiporters. This protein is primarily localized to late endosomes and functions as a chloride/proton antiporter. Alternate splicing results in both coding and non-coding variants. Additional alternately spliced variants have been described but their full-length structure is unknown. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (ClC-6c) lacks an internal coding exon and multiple 3' coding exons, as compared to variant ClC-6a. The resulting isoform ClC-6c has a shorter and distinct C-terminus, as compared to isoform ClC-6a.