GRLF1 (ARHGAP35) (NM_004491) Human Untagged Clone

CAT#: SC308989

ARHGAP35 (untagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP35
• Synonyms: GRF-1; GRLF1; P190-A; P190A; p190ARhoGAP; p190RhoGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004491, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGATGGCAAGAAAGCAAGATGTCCGAATTCCCACCTACAACATCAGTGTGGTGGGATTATCTGGGA
  CCGAGAAGGAAAAGGGCCAGTGTGGGATTGGAAAGTCTTGTTTGTGCAACCGCTTCGTGCGCCCGAGTGC
  TGACGAGTTTCACTTGGACCATACCTCCGTCCTCAGCACCAGTGACTTTGGAGGGCGAGTGGTCAATAAT
  GACCACTTTCTCTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG
  TGGAGCAGACTGAATTTATTGATGATCAGACTTTTCAACCTCATCGAAGCACGGCCCTGCAGCCCTATAT
  CAAGAGAGCTGCTGCGACCAAGCTTGCATCAGCTGAAAAACTCATGTACTTTTGCACTGACCAGCTGGGG
  CTGGAGCAGGACTTTGAGCAGAAACAAATGCCAGACGGAAAGCTGCTGGTTGATGGTTTTCTTCTTGGTA
  TTGATGTTAGCAGGGGCATGAATAGGAACTTTGATGACCAGCTCAAGTTTGTCTCCAATCTCTACAATCA
  GCTTGCAAAAACAAAAAAGCCCATAGTGGTGGTCCTGACTAAGTGTGACGAAGGTGTTGAGCGGTACATT
  AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTGGAGACCTCAGCGAGATCCAATG
  TAAACGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAAAGTCGGGGAAAGACAAAAATCAT
  TCCTTATTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAAGACAAGTATGAGTGGCTG
  GTGAGTCGCATTGTGAAAAACCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATGCAGGCCTCTCCAG
  AATACCAGGACTATGTCTACCTGGAAGGGACTCAGAAAGCCAAGAAGCTGTTTCTACAGCACATCCACCG
  CCTCAAGCATGAGCATATCGAGCGTAGGAGAAAGCTGTACCTGGCAGCCCTGCCATTAGCTTTTGAAGCT
  CTTATACCTAATCTAGATGAAATAGACCACCTAAGCTGCATAAAAGCCAAAAAGCTCTTAGAAACCAAGC
  CAGAATTCTTGAAGTGGTTTGTTGTGCTTGAAGAGACCCCATGGGATGCCACCAGTCACATTGACAACAT
  GGAAAACGAACGGATTCCCTTTGATTTAATGGATACCGTCCCTGCAGAGCAGCTATACGAGGCCCACTTA
  GAGAAGCTGAGGAACGAAAGGAAAAGAGTTGAGATGCGAAGGGCGTTTAAAGAAAACCTGGAGACTTCTC
  CTTTCATAACTCCCGGAAAGCCTTGGGAAGAGGCCCGTAGTTTTATTATGAATGAGGATTTCTACCAGTG
  GCTGGAGGAATCTGTATACATGGATATTTATGGCAAACACCAAAAGCAAATTATAGATAAAGCAAAGGAA
  GAATTTCAGGAGTTGCTTTTGGAATATTCAGAATTGTTTTATGAACTGGAGCTGGATGCTAAGCCCAGCA
  AGGAGAAGATGGGTGTTATTCAGGATGTTCTGGGAGAGGAACAGCGATTTAAAGCATTACAAAAGCTCCA
  AGCAGAGCGTGATGCCCTTATTCTGAAACACATTCATTTTGTGTACCACCCAACAAAGGAGACATGCCCC
  AGCTGCCCAGCTTGTGTGGACGCTAAGATTGAGCACTTGATTAGTTCTCGGTTTATCCGGCCGTCTGACC
  GGAATCAGAAAAATTCACTCTCTGACCCTAACATTGATAGAATCAACTTGGTTATATTGGGCAAAGACGG
  CCTTGCCCGAGAGTTGGCCAATGAGATTCGAGCTCTTTGTACAAATGATGACAAGTATGTGATAGATGGT
  AAAATGTATGAGCTTTCCCTGAGGCCAATAGAGGGGAATGTCAGGCTTCCTGTGAACTCTTTCCAGACGC
  CAACATTTCAGCCCCACGGCTGTCTCTGCCTTTACAATTCAAAGGAATCGCTATCCTATGTAGTGGAAAG
  TATAGAGAAGAGTAGAGAGTCCACGCTGGGCCGGCGGGATAATCATTTAGTCCATCTCCCCCTTACATTA
  ATTTTGGTTAACAAGAGAGGAGACACCAGTGGAGAGACTCTGCATAGCTTAATACAGCAAGGTCAACAAA
  TTGCTAGCAAACTTCAGTGTGTCTTTCTCGACCCTGCTTCTGCTGGCATTGGTTACGGACGCAACATTAA
  TGAAAAGCAAATCAGTCAAGTTTTGAAGGGACTCCTGGACTCTAAGCGTAACTTAAACCTGGTCAGTTCT
  ACTGCTAGCATCAAAGATTTGGCTGATGTTGATCTGCGAATTGTTATGTGTCTGATGTGTGGAGATCCTT
  TTAGTGCAGATGACATACTTTTTCCTGTCCTTCAGTCCCAAACCTGTAAATCTTCCCATTGTGGAAGCAA
  CAACTCTGTTTTACTTGAACTACCAATCGGACTGCACAAGAAGCGGATTGAACTGTCTGTTCTTTCATAC
  CATTCCTCCTTTAGCATCAGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA
  AGGCCTCTTTGGCTATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCTATTCAGCTTGTAGC
  ACTCACTGATGGCGCTGTAGATGTCCTGGACAATGACTTAAGTAGGGAACAGCTAACTGAGGGGGAGGAG
  ATTGCTCAAGAAATTGACGGAAGGTTCACAAGCATCCCCTGTAGCCAACCCCAGCATAAACTTGAGATCT
  TTCACCCATTTTTTAAAGATGTGGTGGAAAAAAAGAACATAATCGAGGCTACTCATATGTACGATAATGC
  TGCCGAGGCCTGTAGCACCACCGAAGAGGTGTTTAACTCCCCCCGGGCAGGATCACCGCTCTGCAACTCA
  AACCTGCAGGATTCAGAAGAAGATATCGAGCCATCTTACAGCCTGTTTCGAGAAGACACATCACTGCCTT
  CTCTGTCCAAAGACCATTCTAAGCTCTCTATGGAACTGGAGGGAAATGATGGGCTGTCTTTCATTATGAG
  CAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCGCCAGTCAAACCAAAGCCTCCTGTCCATTTTGAA
  ATTACAAAGGGGGATCTATCTTATTTAGACCAAGGCCATAGGGATGGACAGAGGAAGTCTGTGTCTTCTA
  GCCCCTGGCTGCCTCAGGATGGGTTTGATCCTTCTGACTATGCTGAACCCATGGATGCTGTGGTGAAGCC
  AAGGAATGAAGAAGAAAACATATACTCCGTGCCCCATGACAGCACCCAAGGCAAAATCATCACCATTCGG
  AATATCAACAAAGCCCAGTCCAACGGCAGCGGGAATGGTTCTGACAGTGAAATGGACACCAGCTCTCTAG
  AGCGAGGGCGCAAGGTTTCCATCGTGAGCAAGCCAGTGCTGTACAGGACGAGATGCACCCGGCTGGGGCG
  GTTTGCTAGTTACCGGACCAGCTTCAGCGTGGGGAGTGATGATGAGCTGGGGCCCATCCGGAAGAAAGAG
  GAGGATCAGGCATCCCAGGGTTATAAAGGGGACAATGCTGTCATTCCATACGAAACAGACGAAGACCCGC
  GGAGGAGGAATATTCTTCGCAGCCTAAGGAGGAACACTAAGAAACCAAAGCCCAAACCCCGGCCATCCAT
  CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACTGTCGTGACTCCAGAGAAGCCG
  ATCCCCATTTTTATTGAAAGATGTATTGAGTACATTGAAGCCACAGGACTGAGCACGGAAGGCATCTACC
  GGGTCAGCGGGAACAAGTCTGAGATGGAGAGTCTGCAGAGACAGTTTGATCAAGACCACAACCTGGACCT
  GGCAGAGAAAGACTTTACGGTGAATACCGTGGCTGGTGCCATGAAGAGCTTTTTCTCAGAACTGCCTGAC
  CCCCTGGTCCCGTATAACATGCAGATCGACTTGGTGGAAGCACACAAAATCAACGACCGGGAGCAGAAGT
  TGCATGCCCTTAAGGAGGTATTAAAGAAATTTCCAAAGGAAAACCACGAAGTCTTCAAGTATGTCATCTC
  TCACCTAAACAAGGTCAGCCACAACAACAAGGTGAATCTCATGACCAGCGAGAACCTCTCCATCTGCTTC
  TGGCCCACCTTGATGAGACCTGATTTCAGCACTATGGACGCCCTCACAGCCACGCGCACCTACCAGACAA
  TCATTGAACTCTTTATCCAGCAGTGCCCCTTCTTCTTCTACAATCGGCCCATCACCGAGCCCCCCGGCGC
  CAGGCCCAGCTCCCCCTCTGCCGTGGCTTCCACCGTCCCCTTCCTCACTTCCACGCCTGTCACAAGTCAG
  CCGTCGCCCCCACAGTCGCCTCCACCCACCCCCCAGTCCCCAATGCAGCCACTGCTTCCCTCCCAGCTTC
  AAGCCGAACACACGCTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004491
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004491.4, NP_004482.4
• RefSeq Size: 8904 bp
• RefSeq ORF: 4500 bp
• Locus ID: 2909
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: 'The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate segment in the coding region compared to variant 1, resulting in a longer isoform (b) that has a distinct C-terminus compared to isoform a.