VR1 (TRPV1) (NM_080706) Human Untagged Clone

CAT#: SC309081

TRPV1 (untagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPV1
• Synonyms: VR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080706, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGAAATGGAGCAGCACAGACTTGGGGGCAGCTGCGGACCCACTCCAAAAGGACACCTGCCCAGACC
  CCCTGGATGGAGACCCTAACTCCAGGCCACCTCCAGCCAAGCCCCAGCTCTCCACGGCCAAGAGCCGCAC
  CCGGCTCTTTGGGAAGGGTGACTCGGAGGAGGCTTTCCCGGTGGATTGCCCTCACGAGGAAGGTGAGCTG
  GACTCCTGCCCGACCATCACAGTCAGCCCTGTTATCACCATCCAGAGGCCAGGAGACGGCCCCACCGGTG
  CCAGGCTGCTGTCCCAGGACTCTGTCGCCGCCAGCACCGAGAAGACCCTCAGGCTCTATGATCGCAGGAG
  TATCTTTGAAGCCGTTGCTCAGAATAACTGCCAGGATCTGGAGAGCCTGCTGCTCTTCCTGCAGAAGAGC
  AAGAAGCACCTCACAGACAACGAGTTCAAAGACCCTGAGACAGGGAAGACCTGTCTGCTGAAAGCCATGC
  TCAACCTGCACGACGGACAGAACACCACCATCCCCCTGCTCCTGGAGATCGCGCGGCAAACGGACAGCCT
  GAAGGAGCTTGTCAACGCCAGCTACACGGACAGCTACTACAAGGGCCAGACAGCACTGCACATCGCCATC
  GAGAGACGCAACATGGCCCTGGTGACCCTCCTGGTGGAGAACGGAGCAGACGTCCAGGCTGCGGCCCATG
  GGGACTTCTTTAAGAAAACCAAAGGGCGGCCTGGATTCTACTTCGGTGAACTGCCCCTGTCCCTGGCCGC
  GTGCACCAACCAGCTGGGCATCGTGAAGTTCCTGCTGCAGAACTCCTGGCAGACGGCCGACATCAGCGCC
  AGGGACTCGGTGGGCAACACGGTGCTGCACGCCCTGGTGGAGGTGGCCGACAACACGGCCGACAACACGA
  AGTTTGTGACGAGCATGTACAATGAGATTCTGATGCTGGGGGCCAAACTGCACCCGACGCTGAAGCTGGA
  GGAGCTCACCAACAAGAAGGGAATGACGCCGCTGGCTCTGGCAGCTGGGACCGGGAAGATCGGGGTCTTG
  GCCTATATTCTCCAGCGGGAGATCCAGGAGCCCGAGTGCAGGCACCTGTCCAGGAAGTTCACCGAGTGGG
  CCTACGGGCCCGTGCACTCCTCGCTGTACGACCTGTCCTGCATCGACACCTGCGAGAAGAACTCGGTGCT
  GGAGGTGATCGCCTACAGCAGCAGCGAGACCCCTAATCGCCACGACATGCTCTTGGTGGAGCCGCTGAAC
  CGACTCCTGCAGGACAAGTGGGACAGATTCGTCAAGCGCATCTTCTACTTCAACTTCCTGGTCTACTGCC
  TGTACATGATCATCTTCACCATGGCTGCCTACTACAGGCCCGTGGATGGCTTGCCTCCCTTTAAGATGGA
  AAAAACTGGAGACTATTTCCGAGTTACTGGAGAGATCCTGTCTGTGTTAGGAGGAGTCTACTTCTTTTTC
  CGAGGGATTCAGTATTTCCTGCAGAGGCGGCCGTCGATGAAGACCCTGTTTGTGGACAGCTACAGTGAGA
  TGCTTTTCTTTCTGCAGTCACTGTTCATGCTGGCCACCGTGGTGCTGTACTTCAGCCACCTCAAGGAGTA
  TGTGGCTTCCATGGTATTCTCCCTGGCCTTGGGCTGGACCAACATGCTCTACTACACCCGCGGTTTCCAG
  CAGATGGGCATCTATGCCGTCATGATAGAGAAGATGATCCTGAGAGACCTGTGCCGTTTCATGTTTGTCT
  ACATCGTCTTCTTGTTCGGGTTTTCCACAGCGGTGGTGACGCTGATTGAAGACGGGAAGAATGACTCCCT
  GCCGTCTGAGTCCACGTCGCACAGGTGGCGGGGGCCTGCCTGCAGGCCCCCCGATAGCTCCTACAACAGC
  CTGTACTCCACCTGCCTGGAGCTGTTCAAGTTCACCATCGGCATGGGCGACCTGGAGTTCACTGAGAACT
  ATGACTTCAAGGCTGTCTTCATCATCCTGCTGCTGGCCTATGTAATTCTCACCTACATCCTCCTGCTCAA
  CATGCTCATCGCCCTCATGGGTGAGACTGTCAACAAGATCGCACAGGAGAGCAAGAACATCTGGAAGCTG
  CAGAGAGCCATCACCATCCTGGACACGGAGAAGAGCTTCCTTAAGTGCATGAGGAAGGCCTTCCGCTCAG
  GCAAGCTGCTGCAGGTGGGGTACACACCTGATGGCAAGGACGACTACCGGTGGTGCTTCAGGGTGGACGA
  GGTGAACTGGACCACCTGGAACACCAACGTGGGCATCATCAACGAAGACCCGGGCAACTGTGAGGGCGTC
  AAGCGCACCCTGAGCTTCTCCCTGCGGTCAAGCAGAGTTTCAGGCAGACACTGGAAGAACTTTGCCCTGG
  TCCCCCTTTTAAGAGAGGCAAGTGCTCGAGATAGGCAGTCTGCTCAGCCCGAGGAAGTTTATCTGCGACA
  GTTTTCAGGGTCTCTGAAGCCAGAGGACGCTGAGGTCTTCAAGAGTCCTGCCGCTTCCGGGGAGAAGTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_080706
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The full-length cDNA was sequenced, and the ORF matches with that of NM_080706. There are 3 SNPs were detected in ORF, and two of these encode different amino acids compared to reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080706.2, NP_542437.2
• RefSeq Size: 4415 bp
• RefSeq ORF: 2520 bp
• Locus ID: 7442
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) represents the longest transcript. Variants 1-4 all encode the same protein.