KCNC4 (NM_153763) Human Untagged Clone

CAT#: SC309151

KCNC4 (untagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 4 (KCNC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNC4
• Synonyms: HKSHIIIC; KSHIIIC; KV3.4; MGC126818
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153763, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCAGCTCGGTGTGTGTCTCCTCCTACCGCGGGCGCAAGTCGGGGAACAAGCCTCCGTCCAAAACAT
  GTCTGAAGGAGGAGATGGCCAAGGGCGAGGCGTCGGAGAAGATCATCATCAACGTGGGCGGCACGCGACA
  TGAGACCTACCGCAGCACCCTGCGCACCCTACCGGGAACCCGCCTCGCCTGGCTGGCCGACCCCGACGGC
  GGGGGCCGGCCCGAGACCGATGGCGGCGGTGTGGGTAGCAGCGGCAGCAGCGGCGGCGGGGGCTGCGAGT
  TCTTCTTCGACAGGCACCCGGGCGTCTTCGCCTACGTGCTCAACTACTACCGCACCGGCAAGCTGCACTG
  CCCCGCGGACGTGTGCGGGCCGCTCTTCGAAGAGGAGCTCACCTTCTGGGGCATCGACGAGACCGACGTG
  GAACCCTGCTGCTGGATGACCTACCGGCAGCACCGCGACGCCGAGGAGGCGCTCGACATCTTCGAGAGCC
  CGGACGGAGGCGGCAGCGGCGCGGGGCCCAGCGACGAGGCCGGCGACGATGAGCGGGAGCTGGCCCTGCA
  GCGACTGGGCCCCCACGAGGGAGGCGCGGGCCATGGCGCCGGGTCTGGGGGCTGCCGCGGCTGGCAGCCC
  CGCATGTGGGCGCTCTTCGAGGATCCCTACTCCTCCCGGGCCGCTAGGGTAGTGGCCTTTGCCTCTCTCT
  TCTTCATCCTGGTCTCCATCACCACTTTCTGCCTGGAGACCCATGAGGCCTTTAATATCGACCGCAACGT
  GACAGAGATCCTCCGCGTAGGGAACATCACCAGCGTGCACTTCCGGCGGGAGGTAGAGACAGAGCCCATC
  CTGACCTACATCGAGGGCGTATGTGTGCTGTGGTTCACACTGGAGTTCCTGGTGCGCATCGTGTGCTGCC
  CCGACACGCTGGACTTCGTCAAGAACCTGCTCAACATCATCGACTTTGTGGCCATCCTGCCCTTCTACCT
  GGAGGTGGGGCTGAGCGGCCTGTCATCCAAGGCGGCCCGCGACGTGCTGGGCTTCCTGCGCGTGGTGCGC
  TTCGTGCGCATCCTGCGTATCTTCAAGCTCACACGCCACTTCGTGGGGCTACGCGTGCTGGGCCACACCC
  TGAGGGCCAGCACCAATGAGTTCCTGCTGCTTATCATCTTCCTGGCCCTGGGTGTGCTCATCTTTGCCAC
  CATGATCTACTACGCTGAGCGCATTGGGGCCAGGCCCTCCGACCCTCGGGGTAATGACCACACCGACTTC
  AAGAACATCCCCATTGGCTTCTGGTGGGCTGTGGTCACCATGACGACACTGGGCTACGGAGACATGTACC
  CCAAGACGTGGTCAGGCATGCTGGTAGGGGCACTGTGTGCACTGGCTGGCGTGCTCACCATCGCCATGCC
  GGTGCCTGTCATCGTCAACAACTTCGGCATGTACTACTCCCTGGCCATGGCCAAGCAGAAGCTGCCCAAG
  AAACGGAAGAAGCACGTGCCACGGCCGGCGCAGCTGGAGTCACCCATGTACTGCAAGTCTGAGGAGACTT
  CCCCCCGGGACAGCACCTGCAGTGATACCAGCCCCCCTGCCCGGGAAGAGGGTATGATCGAGAGGAAACG
  GGCAGGTGAGATTAGGGGTTGGGAAGGAAAATCCCTTTTCCCCCAGTGGCCTAGGGAGTTTCCAAATGGA
  CCTCAGACCTTGGGATTTGGCATGTGTTTTGTGTGGGGCTTCCCTAAGCATAAAGATGTGCCTTTATGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_153763
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153763.2, NP_720198.1
• RefSeq Size: 1855 bp
• RefSeq ORF: 1749 bp
• Locus ID: 3749
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. It generates atypical voltage-dependent transient current that may be important for neuronal excitability. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (2) is missing two 3' end exons found in transcript variant 1, resulting in an isoform (b) that is shorter with a distinct C-terminus compared to isoform a.