IMPDH1 (NM_183243) Human Untagged Clone

CAT#: SC309338

IMPDH1 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPDH1
• Synonyms: IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_183243, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGGCCACTCACTCCACCACCGCTGCAGGGAGGCGGAGCCGCCGCTGTTCCGGAGCCCGGAGCCC
  GGCAACACCCGGGACACGAGACGGCGGCGCAGCGGTACAGCGCCCGACTGCTGCAGGCCGGCTACGAGCC
  CGAGAGCATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTGCCCGAGGATGGGCTCACCGCGCAG
  CAGCTCTTCGCCAGCGCCGACGGCCTCACCTACAACGACTTCCTGATTCTCCCAGGATTCATAGACTTCA
  TAGCTGATGAGGTGGACCTGACCTCAGCCCTGACCCGGAAGATCACGCTGAAGACGCCACTGATCTCCTC
  CCCCATGGACACTGTGACAGAGGCTGACATGGCCATTGCCATGGCTCTGATGGGAGGTATTGGTTTCATT
  CACCACAACTGCACCCCAGAGTTCCAGGCCAACGAGGTGCGGAAGGTCAAGAAGTTTGAACAGGGCTTCA
  TCACGGACCCTGTGGTGCTGAGCCCCTCGCACACTGTGGGCGATGTGCTGGAGGCCAAGATGCGGCATGG
  CTTCTCTGGCATCCCCATCACTGAGACGGGCACCATGGGCAGCAAGCTGGTGGGCATCGTCACCTCCCGA
  GACATCGACTTTCTTGCTGAGAAGGACCACACCACCCTCCTCAGTGAGGTGATGACGCCAAGGATTGAAC
  TGGTGGTGGCTCCAGCAGGTGTGACGTTGAAAGAGGCAAATGAGATCCTGCAGCGTAGCAAGAAAGGGAA
  GCTGCCTATCGTCAATGATTGCGATGAGCTGGTGGCCATCATCGCCCGCACCGACCTGAAGAAGAACCGA
  GACTACCCTCTGGCCTCCAAGGATTCCCAGAAGCAGCTGCTCTGTGGGGCAGCTGTGGGCACCCGTGAGG
  ATGACAAATACCGTCTGGACCTGCTCACCCAGGCGGGCGTCGACGTCATAGTCTTGGACTCGTCCCAAGG
  GAATTCGGTGTATCAGATCGCCATGGTGCATTACATCAAACAGAAGTACCCCCACCTCCAGGTGATTGGG
  GGGAACGTGGTGACAGCAGCCCAGGCCAAGAACCTGATTGATGCTGGTGTGGACGGGCTGCGCGTGGGCA
  TGGGCTGCGGCTCCATCTGCATCACCCAGGAAGTGATGGCCTGTGGTCGGCCCCAGGGCACTGCTGTGTA
  CAAGGTGGCTGAGTATGCCCGGCGCTTTGGTGTGCCCATCATAGCCGATGGCGGCATCCAGACCGTGGGA
  CACGTGGTCAAGGCCCTGGCCCTTGGAGCCTCCACAGTGATGATGGGCTCCCTGCTGGCCGCCACTACGG
  AGGCCCCTGGCGAGTACTTCTTCTCAGACGGGGTGCGGCTCAAGAAGTACCGGGGCATGGGCTCACTGGA
  TGCCATGGAGAAGAGCAGCAGCAGCCAGAAACGATACTTCAGCGAGGGGGATAAAGTGAAGATCGCGCAG
  GGTGTCTCGGGCTCCATCCAGGACAAAGGATCCATTCAGAAGTTCGTGCCCTACCTCATAGCAGGCATCC
  AACACGGCTGCCAGGATATCGGGGCCCGCAGCCTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCT
  CAAGTTTGAGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAAAG
  CGGCTGTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_183243
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183243.2, NP_899066.1
• RefSeq Size: 2517 bp
• RefSeq ORF: 1692 bp
• Locus ID: 3614
• Cytogenetics: 7q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.