PTGER3 (NM_198715) Human Untagged Clone

CAT#: SC309547

PTGER3 (untagged)-Human prostaglandin E receptor 3 (subtype EP3) (PTGER3), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTGER3
• Synonyms: EP3; EP3-I; EP3-II; EP3-III; EP3-IV; EP3-VI; EP3e; PGE2-R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198715 edited
  AGGAAGGCGTGGCTCCCTCCCGGGCCAGTGAGCCCTGGCGCCGCCGCGGCCGCGGTCCCA
  GCAGCGGAGTAGGGCGGCGGCTGCGCCCCGCACCATGGGGGGCAGCCCAGCCCCAGCCGC
  GGTAAACGCCGACCTCCGCCGCCGCCCGCGCCGCGTCTGCCCCCTCCCGCTGCGGCTCTC
  TGGACGCCATCCCCTCCTCACCTCGAAGCCAACATGAAGGAGACCCGGGGCTACGGAGGG
  GATGCCCCCTTCTGCACCCGCCTCAACCACTCCTACACAGGCATGTGGGCGCCCGAGCGT
  TCCGCCGAGGCGCGGGGCAACCTCACGCGCCCTCCAGGGTCTGGCGAGGATTGCGGATCG
  GTGTCCGTGGCCTTCCCGATCACCATGCTGCTCACTGGTTTCGTGGGCAACGCACTGGCC
  ATGCTGCTCGTGTCGCGCAGCTACCGGCGCCGGGAGAGCAAGCGCAAGAAGTCCTTCCTG
  CTGTGCATCGGCTGGCTGGCGCTCACCGACCTGGTCGGGCAGCTTCTCACCACCCCGGTC
  GTCATCGTCGTGTACCTGTCCAAGCAGCGTTGGGAGCACATCGACCCGTCGGGGCGGCTC
  TGCACCTTTTTCGGGCTGACCATGACTGTTTTCGGGCTCTCCTCGTTGTTCATCGCCAGC
  GCCATGGCCGTCGAGCGGGCGCT:GGCCATCAGGGCGCCGCACTGGTATGCGAGCCACAT
  GAAGACGCGTGCCACCCGCGCTGTGCTGCTCGGCGTGTGGCTGGCCGTGCTCGCCTTCGC
  CCTGCTGCCGGTGCTGGGCGTGGGCCAGTACACCGTCCAGTGGCCCGGGACGTGGTGCTT
  CATCAGCACCGGGCGAGGGGGCAACGGGACTAGCTCTTCGCATAACTGGGGCAACCTTTT
  CTTCGCCTCTGCCTTTGCCTTCCTGGGGCTCTTGGCGCTGACAGTCACCTTTTCCTGCAA
  CCTGGCCACCATTAAGGCCCTGGTGTCCCGCTGCCGGGCCAAGGCCACGGCATCTCAGTC
  CAGTGCCCAGTGGGGCCGCATCACGACCGAGACGGCCATTCAGCTTATGGGGATCATGTG
  CGTGCTGTCGGTCTGCTGGTCTCCGCTCCTGATAATGATGTTGAAAATGATCTTCAATCA
  GACATCAGTTGAGCACTGCAAGACACACACGGAGAAGCAGAAAGAATGCAACTTCTTCTT
  AATAGCTGTTCGCCTGGCTTCACTGAACCAGATCTTGGATCCTTGGGTTTACCTGCTGTT
  AAGAAAGATCCTTCTTCGAAAGTTTTGCCAGGTAGCAAATGCTGTCTCCAGCTGCTCTAA
  TGATGGACAGAAAGGGCAGCCTATCTCATTATCTAATGAAATAATACAGACAGAAGCATG
  A
  
• Restriction Sites: Please inquire
• ACCN: NM_198715
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198715.1, NP_942008.1
• RefSeq Size: 2468 bp
• RefSeq ORF: 1167 bp
• Locus ID: 5733
• Cytogenetics: 1p31.1
• Protein Families: Druggable Genome, GPCR, Transcription Factors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]'
  Transcript Variant: This variant (5) lacks multiple 3' exons and has an unique 3' end region when compared to variant 1. The resulting protein (isoform 5) has a distinct and shorter C-terminus, as compared to isoform 1. Other names for this transcript are EP3-II, EP3C, and EP3D.