delta Sarcoglycan (SGCD) (NM_172244) Human Untagged Clone

CAT#: SC309561

SGCD (untagged)-Human sarcoglycan, delta (35kDa dystrophin-associated glycoprotein) (SGCD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGCD
• Synonyms: 35DAG; CMD1L; DAGD; LGMDR6; SG-delta; SGCDP; SGD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172244 edited
  GACAAGAGAGAGACATTACTGCCGGGAGTGTTGAGTGAAGGGACCAGGTGGAGATGATGC
  CTCAGGAGCAGTACACTCACCACCGGAGCACCATGCCTGGCTCTGTGGGGCCACAGGTAT
  ACAAGGTGGGGATTTATGGCTGGCGGAAACGATGCCTGTATTTCTTTGTCCTGCTCCTCA
  TGATTTTAATACTGGTGAACTTGGCCATGACCATCTGGATTCTCAAAGTCATGAACTTCA
  CAATTGATGGAATGGGAAACCTGAGGATCACAGAAAAAGGTCTAAAGCTAGAAGGAGACT
  CTGAATTCTTACAACCTCTCTACGCCAAAGAAATCCAGTCCCAACCAGGTAATGCCCTGT
  ACTTCAAGTCTGCCAGAAATGTTACAGTGAACATTCTCAATGACCAGACTAAAGTGCTAA
  CTCAGCTTATAACAGGTCCAAAAGCCGTAGAAGCTTATGGTAAAAAATTTGAGGTAAAAA
  CTGTTTCTGGAAAATTGCTCTTCTCTGCAGACAATAATGAAGTGGTAGTAGGAGCTGAAA
  GATTACGAGTTTTAGGAGCGGAGGGCACAGTGTTCCCTAAATCTATAGAAACACCTAATG
  TCAGGGCAGACCCCTTCAAAGAACTAAGGTTGGAGTCCCCAACCCGGTCTCTAGTGATGG
  AGGCCCCAAAAGGAGTGGAAATCAATGCAGAAGCTGGCAATATGGAAGCCACCTGCAGGA
  CAGAGCTGAGACTGGAATCCAAAGATGGAGAGGTGAGGGATGAGAAGGACAGAAGTTCAA
  AGAGCTACAGCTTCAACAGGCCAACCCTTCCCATAACTGGTTGACCTCGGAGTTGGATCC
  TACAGTGTATCAACAAAAGGAGCCAAGCAGGTTTTATTTCTGAAACAATTAATTGAGCAG
  CATGATTATAAGCCAAACCCACAATCCATCAAAGTGATGATTTCTTATTTGTAAAATGCA
  GAGATAATGGCATGTATTCCAAGTACAGAATTATATGACCATGAAAATGAATGCTATTTT
  CAAATTCTCTCTTGTCACCTTAAAATAAGATTTTGTTAGCCAACATAATTAAGCTGTATA
  TATTATACACATCTGGCTCAAGATGAACTTAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172244
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_172244.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172244.2, NP_758447.1
• RefSeq Size: 1576 bp
• RefSeq ORF: 771 bp
• Locus ID: 6444
• Cytogenetics: 5q33.2-q33.3
• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
• Gene Summary: 'The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2, also known as SGCD2), contains an alternate 3' terminal exon compared to transcript variant 1. This results in a shorter isoform (2) with a distinct C-terminus compared to isoform 1.