CHD4 (NM_001273) Human Untagged Clone

CAT#: SC309882

CHD4 (untagged)-Human chromodomain helicase DNA binding protein 4 (CHD4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHD4
• Synonyms: CHD-4; Mi-2b; Mi2-BETA; SIHIWES
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001273, the custom clone sequence may differ by one or more nucleotides
  ATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGCTCGGCGGGCAGTGAGGAGGAGGATATG
  GATGCACTTTTGAACAACAGCCTGCCCCCACCCCACCCAGAAAATGAAGAGGACCCAGAA
  GAGGATTTGTCAGAAACAGAGACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAAGAAACCT
  CGGGACCCTAAAATCCCTAAGAGCAAGCGCCAAAAAAAGGAGCGTATGCTCTTATGCCGG
  CAGCTGGGGGACAGCTCTGGGGAGGGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCT
  CTGCGCTCAGACAGTGAGGGCAGCGACTATACTCCTGGCAAGAAGAAGAAGAAGAAGCTT
  GGACCTAAGAAAGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGAGGAGGAGGAGGAGGAT
  GATGATGATGATTCAAAGGAGCCTAAATCATCTGCTCAGCTCCTGGAAGACTGGGGCATG
  GAAGACATTGACCACGTGTTCTCAGAGGAGGATTATCGAACCCTCACCAACTACAAGGCC
  TTCAGCCAGTTTGTCAGACCCCTCATTGCTGCCAAAAATCCCAAGATTGCTGTCTCCAAG
  ATGATGATGGTTTTGGGTGCAAAATGGCGGGAGTTCAGTACCAATAACCCCTTCAAAGGC
  AGTTCTGGGGCATCAGTGGCAGCTGCGGCAGCAGCAGCGGTAGCTGTGGTGGAGAGCATG
  GTGACAGCCACTGAGGTTGCACCACCACCTCCCCCTGTGGAGGTGCCTATCCGCAAGGCC
  AAGACCAAGGAGGGCAAAGGTCCCAATGCTCGGAGGAAGCCCAAGGGCAGCCCTCGTGTA
  CCTGATGCCAAGAAGCCTAAACCCAAGAAAGTAGCTCCCCTGAAAATCAAGCTGGGAGGT
  TTTGGTTCCAAGCGTAAGAGATCCTCGAGTGAGGATGATGACTTAGATGTGGAATCTGAC
  TTCGATGATGCCAGTATCAATAGCTATTCTGTTTCTGATGGTTCCACCAGCCGTAGTAGC
  CGCAGCCGCAAGAAACTCCGAACCACTAAAAAGAAAAAGAAAGGCGAGGAGGAGGTGACT
  GCTGTGGATGGTTATGAGACAGACCACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGT
  GAGATCATCCTGTGTGATACCTGTCCCCGTGCTTACCACATGGTCTGCCTGGATCCCGAC
  ATGGAGAAGGCTCCCGAGGGCAAGTGGAGCTGCCCACACTGCGAGAAGGAAGGCATCCAG
  TGGGAAGCTAAAGAGGACAATTCGGAGGGTGAGGAGATCCTGGAAGAGGTTGGGGGAGAC
  CTCGAAGAGGAGGATGACCACCATATGGAATTCTGTCGGGTCTGCAAGGATGGTGGGGAA
  CTGCTCTGCTGTGATACCTGTCCTTCTTCCTACCACATCCACTGCCTGAATCCCCCACTT
  CCAGAGATCCCCAACGGTGAATGGCTCTGTCCCCGTTGTACGTGTCCAGCTCTGAAGGGC
  AAAGTGCAGAAGATCCTAATCTGGAAGTGGGGTCAGCCACCATCTCCCACACCAGTGCCT
  CGGCCTCCAGATGCTGATCCCAACACGCCCTCCCCAAAGCCCTTGGAGGGGCGGCCAGAG
  CGGCAGTTCTTTGTGAAATGGCAAGGCATGTCTTACTGGCACTGCTCCTGGGTTTCTGAA
  CTGCAGCTGGAGCTGCACTGTCAGGTGATGTTCCGAAACTATCAGCGGAAGAATGATATG
  GATGAGCCACCTTCTGGGGACTTTGGTGGTGATGAAGAGAAAAGCCGAAAGCGAAAGAAC
  AAGGACCCTAAATTTGCAGAGATGGAGGAACGCTTCTATCGCTATGGGATAAAACCCGAG
  TGGATGATGATCCACCGAATCCTCAACCACAGTGTGGACAAGAAGGGCCACGTCCACTAC
  TTGATCAAGTGGCGGGACTTACCTTACGATCAGGCTTCTTGGGAGAGTGAGGATGTGGAG
  ATCCAGGATTACGACCTGTTCAAGCAGAGCTATTGGAATCACAGGGAGTTAATGAGGGGT
  GAGGAAGGCCGACCAGGCAAGAAGCTCAAGAAGGTGAAGCTTCGGAAGTTGGAGAGGCCT
  CCAGAAACGCCAACAGTTGATCCAACAGTGAAGTATGAGCGACAGCCAGAGTACCTGGAT
  GCTACAGGTGGAACCCTGCACCCCTATCAAATGGAGGGCCTGAATTGGTTGCGCTTCTCC
  TGGGCTCAGGGCACTGACACCATCTTGGCTGATGAGATGGGCCTTGGGAAAACTGTACAG
  ACAGCAGTCTTCCTGTATTCCCTTTACAAGGAGGGTCATTCCAAAGGCCCCTTCCTAGTG
  AGCGCCCCTCTTTCTACCATCATCAACTGGGAGCGGGAGTTTGAAATGTGGGCTCCAGAC
  ATGTATGTCGTAACCTATGTGGGTGACAAGGACAGCCGTGCCATCATCCGAGAGAATGAG
  TTCTCCTTTGAAGACAATGCCATTCGTGGTGGCAAGAAGGCCTCCCGCATGAAGAAAGAG
  GCATCTGTGAAATTCCATGTGCTGCTGACATCCTATGAATTGATCACCATTGACATGGCT
  ATTTTGGGCTCTATTGATTGGGCCTGCCTCATCGTGGATGAAGCCCATCGGCTGAAGAAC
  AATCAGTCTAAGTTCTTCCGGGTATTGAATGGTTACTCACTCCAGCACAAGCTGTTGCTG
  ACTGGGACACCATTACAAAACAATCTGGAAGAGTTGTTTCATCTGCTCAACTTTCTCACC
  CCCGAGAGGTTCCACAATTTGGAAGGTTTTTTGGAGGAGTTTGCTGACATTGCCAAGGAG
  GACCAGATAAAAAAACTGCATGACATGCTGGGGCCGCACATGTTGCGGCGGCTCAAAGCC
  GATGTGTTCAAGAACATGCCCTCCAAGACAGAACTAATTGTGCGTGTGGAGCTGAGCCCT
  ATGCAGAAGAAATACTACAAGTACATCCTCACTCGAAATTTTGAAGCACTCAATGCCCGA
  GGTGGTGGCAACCAGGTGTCTCTGCTGAATGTGGTGATGGATCTTAAGAAGTGCTGCAAC
  CATCCATACCTCTTCCCTGTGGCTGCAATGGAAGCTCCTAAGATGCCTAATGGCATGTAT
  GATGGCAGTGCCCTAATCAGAGCATCTGGGAAATTATTGCTGCTGCAGAAAATGCTCAAG
  AACCTTAAGGAGGGTGGGCATCGTGTACTCATCTTTTCCCAGATGACCAAGATGCTAGAC
  CTGCTAGAGGATTTCTTGGAACATGAAGGTTATAAATACGAACGCATCGATGGTGGAATC
  ACTGGGAACATGCGGCAAGAGGCCATTGACCGCTTCAATGCACCGGGTGCTCAGCAGTTC
  TGCTTCTTGCTTTCCACTCGAGCTGGGGGCCTTGGAATCAATCTGGCCACTGCTGACACA
  GTTATTATCTATGACTCTGACTGGAACCCCCATAATGACATTCAGGCCTTTAGCAGAGCT
  CACCGGATTGGGCAAAATAAAAAGGTAATGATCTACCGGTTTGTGACCCGTGCGTCAGTG
  GAGGAGCGCATCACGCAGGTGGCAAAGAAGAAAATGATGCTGACGCATCTAGTGGTGCGG
  CCTGGGCTGGGCTCCAAGACTGGATCTATGTCCAAACAGGAGCTTGATGATATCCTCAAA
  TTTGGCACTGAGGAACTATTCAAGGATGAAGCCACTGATGGAGGAGGAGACAACAAAGAG
  GGAGAAGATAGCAGTGTTATCCACTACGATGATAAGGCCATTGAACGGCTGCTAGACCGT
  AACCAGGATGAGACTGAAGACACAGAATTGCAGGGCATGAATGAATATTTGAGCTCATTC
  AAAGTGGCCCAGTATGTGGTACGGGAAGAAGAAATGGGGGAGGAAGAGGAGGTAGAACGG
  GAAATCATTAAACAGGAAGAAAGTGTGGATCCTGACTACTGGGAGAAATTGCTGCGGCAC
  CATTATGAGCAGCAGCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAAAGAATCCGT
  AAACAGGTCAACTACAATGATGGCTCCCAGGAGGACCGAGATTGGCAGGACGACCAGTCC
  GACAACCAGTCCGATTACTCAGTGGCTTCAGAGGAAGGTGATGAAGACTTTGATGAACGT
  TCAGAAGCTCCCCGTAGGCCCAGTCGTAAGGGCCTGCGGAATGATAAAGATAAGCCATTG
  CCTCCTCTGTTGGCCCGTGTTGGTGGGAATATTGAAGTACTTGGTTTTAATGCTCGTCAG
  CGAAAAGCCTTTCTTAATGCAATTATGCGATATGGTATGCCACCTCAGGATGCTTTTACT
  ACCCAGTGGCTTGTAAGAGACCTGCGAGGCAAATCAGAGAAAGAGTTCAAGGCATATGTC
  TCTCTTTTCATGCGGCATTTATGTGAGCCGGGGGCAGATGGGGCTGAGACCTTTGCTGAT
  GGTGTCCCCCGAGAAGGCCTGTCTCGCCAGCATGTCCTTACTAGAATTGGTGTTATGTCT
  TTGATTCGCAAGAAGGTTCAGGAGTTTGAACATGTTAATGGGCGCTGGAGCATGCCTGAA
  CTGGCTGAGGTGGAGGAAAACAAGAAGATGTCCCAGCCAGGGTCACCCTCCCCAAAAACT
  CCTACACCCTCCACTCCAGGGGACACGCAGCCCAACACTCCTGCACCTGTCCCACCTGCT
  GAAGATGGGATAAAAATAGAGGAAAATAGCCTCAAAGAAGAAGAGAGCATAGAAGGAGAA
  AAGGAGGTTAAATCTACAGCCCCTGAGACTGCCATTGAGTGTACACAGGCCCCTGCCCCT
  GCCTCAGAGGATGAAAAGGTCGTTGTTGAACCCCCTGAGGGAGAGGAGAAAGTGGAAAAG
  GCAGAGGTGAAGGAGAGAACAGAGGAACCTATGGAGACAGAGCCCAAAGGTGCTGCTGAT
  GTAGAGAAGGTGGAGGAAAAGTCAGCAATAGATCTGACCCCTATTGTGGTAGAAGACAAA
  GAAGAGAAGAAAGAAGAAGAAGAGAAAAAAGAGGTGATGCTTCAGAATGGAGAGACCCCC
  AAGGACCTGAATGATGAGAAACAGAAGAAAAATATTAAACAACGTTTCATGTTTAACATT
  GCAGATGGTGGTTTTACTGAGTTGCACTCCCTTTGGCAGAATGAAGAGCGGGCAGCCACA
  GTTACCAAGAAGACTTATGAGATCTGGCATCGACGGCATGACTACTGGCTGCTAGCCGGC
  ATTATAAACCATGGCTATGCCCGGTGGCAAGACATCCAGAATGACCCACGCTATGCCATC
  CTCAATGAGCCTTTCAAGGGTGAAATGAACCGTGGCAATTTCTTAGAGATCAAGAATAAA
  TTTCTAGCTCGAAGGTTTAAGCTCTTAGAACAAGCTCTGGTGATTGAGGAACAGCTGCGC
  CGGGCTGCTTACTTGAACATGTCAGAAGACCCTTCTCACCCTTCCATGGCCCTCAACACC
  CGCTTTGCTGAGGTGGAGTGTTTGGCGGAAAGTCATCAGCACCTGTCCAAGGAGTCAATG
  GCAGGAAACAAGCCAGCCAATGCAGTCCTGCACAAAGTTCTGAAACAGCTGGAAGAACTG
  CTGAGTGACATGAAAGCTGATGTGACTCGACTCCCAGCTACCATTGCCCGAATTCCCCCA
  GTTGCTGTGAGGTTACAGATGTCAGAGCGTAACATTCTCAGCCGCCTGGCAAACCGGGCA
  CCCGAACCTACCCCACAGCAGGTAGCCCAGCAGCAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001273
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001273.2, NP_001264.2
• RefSeq Size: 6511 bp
• RefSeq ORF: 5739 bp
• Locus ID: 1108
• Cytogenetics: 12p13.31
• Domains: SNF2_N, CHROMO, DEAD, helicase_C, PHD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).