TJP1 (NM_175610) Human Untagged Clone
CAT#: SC309896
TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 2
"NM_175610" in other vectors (2)
SC309896 is the updated version of SC120781.
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | TJP1 |
Synonyms | ZO-1 |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_175610, the custom clone sequence may differ by one or more nucleotides
ATGTCCGCCAGAGCTGCGGCCGCCAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAA CAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGT GGACGAGATAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTG AAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTTGCAATGGTTAACGGA GTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGGAAAAGTGGGAAA AATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTAAGTCGTCCTGAT CCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGATCCAAGA AGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGT GCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAG CCTGCTAAACCTACTAAAGTCACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTT CGATTGGCAAGCCATATATTTGTTAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGAT GGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATGGTACTGTGACAGAAAATATG TCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTAAAAATGGTAGTT CAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGACAGCATCCACTCT GCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATTCT GGTCGATCACACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCA GAGCCTTCTGATCATTCCAGGCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGT AGAGATGAAGAGAGAATTTCTAAACCTGGGGCTGTCTCAACTCCTGTAAAGCATGCTGAT GATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAAGAAATGAGAAACAAACACCT TCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGATGTGGATTTACCT GTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGGATTCTTCGGCCC AGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTGGA AATGATGTTGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGC TTAGAGGAAGGTGATCAAATTCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGA GAAGAAGCCGTCCTTTTCCTGCTTGACCTCCCTAAAGGAGAAGAAGTGACCATATTGGCT CAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAGATGTAGGAGATTCTTTCTAT ATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGTTTTAACAAAGGA GAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCTTGGCTTGCTATT CGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCT GAGCAGCTAGCCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGAC TTCTGGAGATTCAGAGGTCTTCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGAT TTGTCCGCTCAGCCTGTTCAAACAAAGTTTCCAGCTTATGAAAGAGTGGTTCTTCGAGAA GCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAGCTGATGTTGCCAGAGAAAAG CTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCACGAGACGCTGGA ACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAAATCATAGATCAA GACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCCAG TGGTATCCAATTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGA ATGAGGTTATGTCCAGAATCTCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAA CTTCGTAAAAATAATCACCATCTTTTTACAACTACAATTAACTTAAATTCAATGAATGAT GGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGCAAAACCAGCTGGTATGGGTT TCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCTG TCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGACAGTAGACACACT TCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAA ACTCTTAATGATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCT GTAAGAGAGGACTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTCACCA CAAGCGCAGCCACAACCAATTCATAGAATAGACTCCCCTGGATTTAAGCCAGCCTCTCAA CAGGTGTATAGAAAGGATCCATATCCCGAGGAAATGATGAGGCAGAACCATGTTTTGAAA CAGCCAGCCGTTAGTCACCCAGGGCACAGGCCAGACAAAGAGCCTAATCTGACCTATGAA CCCCAACTCCCATACGTAGAGAAACAAGCCAGCAGAGACCTCGAGCAGCCCACATACAGA TACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACTATGAACATCGTCTGCGATAC GAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAAACAGCCCTAC CCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCC GAAGAGTCCTCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTAC GACAGCAGACCACGTTACGAACAGGCACCTAGAGCATCCGCCCTGCGGCACGAAGAGCAG CCAGCTCCTGGGTATGACACACATGGTAGACTCAGACCGGAAGCCCAGCCCCACCCTTCA GCAGGGCCCAAGCCTGCAGAGTCCAAGCAGTATTTTGAGCAATATTCACGCAGTTACGAG CAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATTTTGAGCCTCTCCATGGTGCT GCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCTGCCTTCAAAC ACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAG CCACAGTCTGTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAG ACCAAGAAGGATGTAAATGACACTGGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCT TCAGGTGCTCCCATCATTGGTCCCAAACCCACTTCTCAGAATCAATTCAGTGAACATGAC AAAACTCTGTACAGGATCCCAGAACCTCAAAAACCTCAACTGAAGCCACCTGAAGATATT GTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAATATTATCGAAAACAGCTGTCA TACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGCCAGCCATCTC TCCGAGCCTGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCA AAGGGAAAGCCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAA CCCATCCAGGCCACTCCCCCTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAG CCTGTCACCAGCGCGTCTCTCCACATACATTCTAAGGGAGCACATGGTGAAGGTAATTCA GTGTCATTGGATTTTCAGAATTCCTTAGTGTCCAAACCAGACCCACCTCCATCTCAGAAT AAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTGCTCAGGCAGCTTTCTATCCC CAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCAGAAAACAGTC ACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAA CGCAAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAA CCTGACTTGTCTTCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTG GCACAGCCTCCTGAGTTTGACAGTGGAGTTGAAACTTTCTCTATCCATGCAGAGAAGCCT AAATATCAAATAAATAATATCAGCACAGTGCCTAAAGCTATTCCTGTGAGTCCTTCAGCT GTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGGCCACAGCCCGAGGCATATTT AACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAATTATCCCTCAA GGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGC ATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGT GGTCCCCATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGATCCT AAAACCTGGCAAAACAAGTGTCTTCCCGGAGATCCAAATTATCTCGTTGGAGCAAACTGT GTTTCTGTCCTTATTGACCACTTTTAA |
Restriction Sites | Please inquire |
ACCN | NM_175610 |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175610.1, NP_783297.1 |
RefSeq Size | 6931 bp |
RefSeq ORF | 5007 bp |
Locus ID | 7082 |
Cytogenetics | 15q13.1 |
Protein Families | Druggable Genome |
Protein Pathways | Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection |
Gene Summary | 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]' Transcript Variant: This variant (2, also known as ZO-1alpha-) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Isoform b is found in both endothelial cells and the highly specialized epithelial junctions of renal glomeruli and sertoli cells of the seminiferous tubules. The exon combination of this variant is inferred based on partial transcript alignments and data in PMID:1590354. |
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