NEK1 (NM_012224) Human Untagged Clone
CAT#: SC309937
NEK1 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2
"NM_012224" in other vectors (7)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | NEK1 |
Synonyms | ALS24; NY-REN-55; SRPS2; SRPS2A; SRTD6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NCBI ORF sequence for NM_012224, the custom clone sequence may differ by one or more nucleotides
ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCCATTCTTGTTAAATCTA CAGAAGATGGCAGACAGTATGTTATCAAGGAAATTAACATCTCAAGAATGTCCAGTAAAGAAAGAGAAGA ATCAAGGAGAGAAGTTGCAGTATTGGCAAACATGAAGCATCCAAATATTGTCCAGTATAGAGAATCATTT GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAGGGAGGGGATCTGTTTAAGCGAATAAATG CTCAGAAAGGCGTTTTGTTTCAAGAGGATCAGATTTTGGACTGGTTTGTACAGATATGTTTGGCCCTGAA ACATGTACATGATAGAAAAATTCTTCATCGAGACATTAAATCTCAGAACATATTTTTAACTAAAGATGGA ACAGTACAACTTGGAGATTTTGGAATTGCTAGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGGACCCCATACTACTTGTCACCTGAAATCTGTGAAAACAAACCTTACAATAATAAAAGTGACATTTG GGCTCTGGGGTGTGTCCTTTATGAGCTGTGTACACTTAAACATGCTTTTGAAGCTGGCAGTATGAAAAAC CTGGTACTGAAGATAATATCTGGATCTTTTCCACCTGTGTCTTTGCATTATTCCTATGATCTCCGCAGTT TGGTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTT TATAGCCAAACGCATTGAAAAGTTTCTCTCTCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACATTT TCGAAGTTTGGATCACAGCCTATACCAGCTAAAAGACCAGCTTCAGGACAAAACTCGATTTCTGTTATGC CTGCTCAGAAAATTACAAAGCCTGCCGCTAAATATGGAATACCTTTAGCATATAAGAAATATGGAGATAA AAAATTACACGAAAAGAAACCACTGCAAAAACATAAACAGGCCCATCAAACTCCAGAGAAGAGAGTGAAT ACTGGAGAAGAAAGGAGGAAAATATCTGAGGAAGCAGCAAGAAAGAGAAGGCTGGAATTTATTGAAAAAG AAAAGAAACAAAAGGATCAGATTATTAGTTTAATGAAGGCTGAACAAATGAAAAGGCAAGAAAAGGAAAG GTTGGAAAGAATAAATAGGGCCAGGGAACAAGGATGGAGAAATGTGCTAAGTGCTGGTGGAAGTGGTGAA GTAAAGGCTCCTTTTCTGGGCAGTGGAGGGACTATAGCTCCATCATCTTTTTCTTCTCGAGGACAGTATG AACATTACCATGCCATTTTTGACCAAATGCAGCAACAAAGAGCAGAAGATAATGAAGCTAAATGGAAAAG AGAAATATATGGTCGAGGTCTTCCAGAAAGAGGAATTCTGCCTGGAGTTCGTCCAGGATTTCCTTATGGG GCTGCAGGTCATCACCATTTTCCTGATGCTGATGATATTAGAAAAACTTTGAAAAGATTGAAGGCGGTGT CTAAACAAGCCAATGCAAACAGGCAAAAAGGGCAGCTAGCTGTAGAAAGAGCTAAACAAGTAGAAGAGTT CCTGCAGCGAAAACGGGAAGCTATGCAGAATAAAGCTCGAGCCGAAGGACATATGGTTTATCTGGCAAGA CTGAGGCAAATAAGACTACAGAATTTCAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGAAAAGA AAGAAGCTAATCATTCTGAAGGACAAGAAGGAAGTGAAGAGGCTGACATGAGGCGCAAAAAAATCGAATC ACTGAAGGCCCATGCAAATGCACGTGCTGCTGTACTAAAAGAACAACTAGAACGAAAGAGAAAGGAGGCT TATGAGAGAGAAAAAAAAGTGTGGGAAGAGCATTTGGTGGCTAAAGGAGTTAAGAGTTCTGATGTTTCTC CACCTTTGGGACAGCATGAAACAGGTGGCTCTCCATCAAAGCAACAGATGAGATCTGTTATTTCTGTAAC TTCAGCTTTGAAAGAAGTTGGCGTGGACAGTAGTTTAACTGATACCCGGGAAACTTCAGAAGAGATGCAA AAGACCAACAATGCTATTTCAAGTAAGCGAGAAATACTTCGTAGATTAAATGAAAATCTTAAAGCTCAAG AAGATGAAAAAGGAAAGCAGAATCTCTCTGATACTTTTGAGATAAATGTTCATGAAGATGCCAAAGAGCA TGAAAAAGAAAAATCAGTTTCATCTGATCGCAAGAAGTGGGAGGCAGGAGGTCAACTTGTGATTCCTCTG GATGAGTTAACACTAGATACATCCTTCTCTACAACTGAAAGACATACAGTGGGAGAAGTTATTAAATTAG GTCCTAATGGATCTCCAAGAAGAGCCTGGGGGAAAAGTCCGACAGATTCTGTTCTAAAGATACTTGGAGA AGCTGAACTACAACTTCAGACAGAACTATTAGAAAATACAACTATTAGAAGTGAGATTTCTCCCGAAGGG GAAAAGTACAAACCCTTAATTACTGGAGAAAAAAAAGTACAATGTATTTCACATGAAATAAACCCATCAG CTATTGTTGATTCTCCTGTTGAGACAAAAAGTCCCGAGTTCAGTGAGGCATCTCCACAGATGTCATTGAA ACTGGAAGGAAATTTAGAAGAACCTGATGATTTGGAAACAGAAATTCTACAAGAGCCAAGTGGAACAAAC AAAGATGAGAGCTTGCCATGCACTATTACTGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTC AGTCGGCAGATAGGATCACCATTCAGGAAAATGAAGTTTCTGAAGATGGAGTCTCGAGTACTGTGGACCA ACTTAGTGACATTCATATAGAGCCTGGAACCAATGATTCTCAGCACTCTAAATGTGATGTAGATAAGTCT GTGCAACCGGAACCATTTTTCCATAAGGTGGTTCATTCTGAACACTTGAACTTAGTCCCTCAAGTTCAAT CAGTTCAGTGTTCACCAGAAGAATCCTTTGCATTTCGATCTCACTCGCATTTACCACCAAAAAATAAAAA CAAGAATTCCTTGCTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAACCCAAAGATGTTAAGGACA TGTTCACTTCCAGATCTCTCAAAGCTGTTCAGAACCCTTATGGATGTTCCCACCGTAGGAGATGTTCGTC AAGACAATCTTGAAATAGATGAAATTGAAGATGAAAACATTAAAGAAGGACCTTCTGATTCTGAAGACAT TGTGTTTGAAGAAACTGACACAGATTTACAAGAGCTGCAGGCCTCGATGGAACAGTTACTTAGGGAACAA CCTGGTGAAGAATACAGTGAAGAAGAAGAGTCAGTCTTGAAGAACAGTGATGTGGAGCCAACTGCAAATG GGACAGATGTGGCAGATGAAGATGACAATCCCAGCAGTGAAAGTGCCCTGAACGAAGAATGGCACTCAGA TAACAGTGATGGTGAAATTGCTAGTGAATGTGAATGCGATAGTGTCTTTAACCATTTAGAGGAACTGAGA CTTCATCTGGAGCAGGAAATGGGCTTTGAAAAATTCTTTGAGGTTTATGAGAAAATAAAGGCTATTCATG AAGATGAAGATGAAAATATTGAAATTTGTTCAAAAATAGTTCAAAATATTTTGGGAAATGAACATCAGCA TCTTTATGCCAAGATTCTTCATTTAGTCATGGCAGATGGAGCCTACCAAGAAGATAATGATGAATAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_012224 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012224.2, NP_036356.1 |
RefSeq Size | 5689 bp |
RefSeq ORF | 3777 bp |
Locus ID | 4750 |
Cytogenetics | 4q33 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | 'The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]' Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC323341 | NEK1 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1) |
USD 2,550.00 |
|
RC221729 | NEK1 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
USD 1,010.00 |
|
RG221729 | NEK1 (GFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2 |
USD 1,110.00 |
|
RC221729L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
USD 1,500.00 |
|
RC221729L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
USD 1,210.00 |
|
RC221729L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, Myc-DDK-tagged |
USD 1,210.00 |
|
RC221729L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2, mGFP tagged |
USD 1,210.00 |
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