Ankyrin G (ANK3) (NM_001149) Human Untagged Clone

CAT#: SC310013

ANK3 (untagged)-Human ankyrin 3, node of Ranvier (ankyrin G) (ANK3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANK3
• Synonyms: ANKYRIN-G; MRT37
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001149, the custom clone sequence may differ by one or more nucleotides
  ATGGCTTTACCGCAGAGTGAAGATGCAATGACCGGGGACACAGACAAATATCTTGGGCCA
  CAGGACCTTAAGGAATTGGGTGATGATTCCCTGCCTGCAGAGGGTTACATGGGCTTTAGT
  CTCGGAGCGCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCTTACACCTTGAAC
  AGAAGCTCCTATGCACGGGACAGCATGATGATTGAAGAACTCCTTGTGCCATCCAAAGAG
  CAGCATCTAACATTCACAAGGGAATTTGATTCAGATTCTCTTAGACATTACAGCTGGGCT
  GCAGACACCTTAGACAATGTCAATCTTGTTTCAAGCCCCATTCATTCTGGGTTTCTGGTT
  AGCTTTATGGTGGACGCGAGAGGGGGCTCCATGAGAGGAAGCCGTCATCACGGGATGAGA
  ATCATCATTCCTCCACGCAAGTGTACGGCCCCCACTCGAATCACCTGCCGTTTGGTAAAG
  AGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAGCCAGTAGGCTG
  GTAGAAATGGGTCCTGCAGGGGCACAATTTTTAGGCCCTGTCATAGTGGAAATCCCTCAC
  TTTGGGTCCATGAGAGGAAAAGAGAGAGAACTCATTGTTCTTCGAAGTGAAAATGGTGAA
  ACTTGGAAGGAGCATCAGTTTGACAGCAAAAATGAAGATTTAACCGAGTTACTTAATGGC
  ATGGATGAAGAACTTGATAGCCCAGAAGAGTTAGGGAAAAAGCGTATCTGCAGGATTATC
  ACGAAAGATTTCCCCCAGTATTTTGCAGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAG
  ATTGGTCCTGAAGGTGGAATTCTGAGCAGCACCACAGTGCCCCTTGTTCAAGCATCTTTC
  CCAGAGGGTGCCCTAACTAAAAGAATTCGAGTGGGCCTCCAGGCCCAGCCTGTTCCAGAT
  GAAATTGTGAAAAAGATCCTTGGAAACAAAGCAACTTTTAGCCCAATTGTCACTGTGGAA
  CCAAGAAGACGGAAATTCCATAAACCAATCACAATGACCATTCCGGTGCCCCCGCCCTCA
  GGAGAAGGTGTATCCAATGGATACAAAGGGGACACTACACCCAATCTGCGTCTTCTCTGT
  AGCATTACAGGGGGCACTTCGCCTGCTCAGTGGGAAGACATCACAGGAACAACTCCTTTG
  ACGTTTATAAAAGATTGTGTCTCCTTTACAACCAATGTTTCAGCCAGATTTTGGCTTGCA
  GACTGCCATCAAGTTTTAGAAACTGTGGGGTTAGCCACGCAACTGTACAGAGAATTGATA
  TGTGTTCCATATATGGCCAAGTTTGTTGTTTTTGCCAAAATGAATGATCCCGTAGAATCT
  TCCTTGCGATGTTTCTGCATGACAGATGACAAAGTGGACAAAACTTTAGAGCAACAAGAG
  AATTTTGAGGAAGTCGCAAGAAGCAAAGATATTGAGGTTCTGGAAGGAAAACCTATTTAT
  GTTGATTGTTATGGAAATTTGGCCCCACTTACCAAAGGAGGACAGCAACTTGTTTTTAAC
  TTTTATTCTTTCAAAGAAAATAGACTGCCATTTTCCATCAAGATTAGAGACACCAGCCAA
  GAGCCCTGTGGTCGTCTGTCTTTTCTGAAAGAACCAAAGACAACAAAAGGACTGCCTCAA
  ACAGCGGTTTGCAACTTAAATATCACTCTGCCAGCACATAAAAAGATTGAGAAAACAGAT
  AGACGACAGAGCTTCGCATCCTTAGCTTTACGTAAGCGCTACAGCTACTTGACTGAGCCT
  GGAATGAGTCCACAGAGTCCATGTGAACGGACAGATATCAGGATGGCAATAGTAGCCGAT
  CACCTGGGACTTAGTTGGACAGAACTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATC
  AATCAAATACGTGTGGAAAATCCAAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAA
  AAATGGGTTACCAGAGACGGAAAAAATGCCACAACTGATGCCTTAACTTCGGTCTTGACA
  AAAATTAATCGAATAGATATAGTGACACTGCTAGAAGGACCAATATTTGATTATGGAAAT
  ATTTCAGGCACCAGAAGTTTTGCAGATGAGAACAATGTTTTCCATGACCCTGTTGATGGT
  TATCCTTCCCTTCAAGTGGAACTGGAAACCCCCACAGGGTTGCACTACACACCACCTACC
  CCTTTCCAGCAAGATGATTATTTTAGTGATATCTCTAGCATAGAATCTCCCCTTAGAACC
  CCTAGTAGACTGAGTGATGGGCTAGTGCCTTCCCAGGGGAACATAGAGCATTCCGCAGAT
  GGACCTCCAGTCGTAACTGCAGAAGACGCTTCCTTAGAAGACAGCAAACTGGAAGACTCA
  GTGCCTTTAACAGAAATGCCTGAAGCAGTGGATGTAGATGAGAGCCAGTTGGAGAATGTA
  TGTCTGAGTTGGCAGAATGAGACATCAAGTGGAAACCTAGAGTCCTGCGCTCAAGCTCGA
  AGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTGACCAGTGTAGAGATTCC
  ATTACCTCATATCTCAAAGGAGAAGCTGGCAAATTTGAAGCAAATGGAAGCCATACAGAA
  ATCACTCCAGAAGCAAAGACAAAATCTTACTTTCCAGAATCCCAAAATGATGTAGGAAAA
  CAGAGTACCAAGGAAACTCTGAAACCAAAAATACATGGATCTGGTCATGTTGAAGAACCA
  GCATCACCACTAGCAGCATATCAGAAATCTCTAGAAGAAACCAGCAAGCTTATAATAGAA
  GAGACTAAACCCTGTGTGCCTGTCAGTATGAAAAAGATGAGTAGGACTTCTCCAGCAGAT
  GGCAAGCCAAGGCTTAGCCTCCATGAAGAAGAGGGGTCCAGTGGGTCTGAGCAAAAGCAG
  GGAGAAGGTTTTAAGGTGAAAACGAAGAAAGAAATCCGGCATGTGGAAAAGAAGAGCCAC
  TCGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001149
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001149.2, NP_001140.2
• RefSeq Size: 5072 bp
• RefSeq ORF: 3006 bp
• Locus ID: 288
• Cytogenetics: 10q21.2
• Domains: ZU5, ANK
• Protein Families: Druggable Genome
• Gene Summary: 'Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) has multiple differences in the 5' UTR and coding sequence compared to variant 1, resulting in an isoform (2) which is considerably shorter and different compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.