Amphiphysin (AMPH) (NM_139316) Human Untagged Clone

CAT#: SC310194

AMPH (untagged)-Human amphiphysin (AMPH), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMPH
• Synonyms: AMPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139316, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACATCAAGACGGGCATCTTCGCCAAGAACGTCCAGAAGCGACTCAACCGCGCGCAGGAAAAGG
  TCCTCCAAAAGCTGGGGAAAGCTGATGAGACAAAAGACGAACAGTTCGAAGAATATGTCCAGAACTTCAA
  ACGGCAAGAAGCAGAGGGTACCAGACTTCAGCGAGAACTCCGAGGATATTTAGCAGCAATCAAAGGCATG
  CAGGAGGCCTCCATGAAGCTCACAGAGTCGCTGCATGAAGTCTATGAGCCTGACTGGTATGGGCGGGAAG
  ATGTGAAAATGGTTGGTGAGAAATGTGATGTGCTGTGGGAAGACTTCCATCAAAAACTCGTGGATGGGTC
  CTTGCTAACACTGGATACCTACCTGGGGCAATTTCCTGACATAAAGAATCGCATCGCCAAGCGCAGCAGG
  AAGCTAGTGGACTATGACAGTGCCCGCCACCATCTGGAAGCTCTGCAGAGCTCCAAGAGGAAGGATGAGA
  GTCGAATCTCTAAGGCAGAAGAAGAATTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTTAACGTTGACTT
  ACAAGAAGAGTTACCATCATTATGGTCAAGACGAGTTGGATTTTATGTTAATACTTTCAAAAACGTCTCC
  AGCCTTGAAGCCAAGTTTCATAAGGAAATTGCGGTGCTTTGCCACAAACTGTATGAAGTGATGACAAAAC
  TGGGTGACCAGCACGCCGACAAGGCCTTCACCATCCAAGGAGCGCCCAGTGATTCGGGTCCTCTCCGCAT
  TGCAAAGACACCATCACCGCCTGAGGAGCCTTCACCCCTCCCGAGCCCGACAGCAAGTCCAAATCATACA
  TTAGCACCTGCGTCTCCCGCACCAGCACGGCCTCGGTCACCTTCACAGACAAGGAAAGGGCCTCCTGTCC
  CACCTCTACCTAAAGTCACCCCGACAAAGGAACTGCAGCAGGAGAACATCATCAGTTTCTTTGAGGACAA
  CTTTGTTCCAGAAATCAGTGTGACAACACCTTCCCAGAATGAAGTCCCTGAGGTGAAGAAAGAGGAGACT
  TTGCTGGATCTGGACTTTGATCCTTTCAAGCCCGAGGTGACACCTGCAGGTTCTGCTGGAGTGACCCACT
  CACCCATGTCTCAGACATTGCCCTGGGACCTATGGACGACAAGCACTGATTTGGTACAGCCGGCTTCTGG
  TGGTTCATTTAATGGATTCACACAGCCCCAGGATACTTCATTATTCACAATGCAGACAGACCAGAGTATG
  ATCTGCAACTTGATCATACCTGGAGCTGATGCTGATGCAGCTGTTGGAACCTTGGTGTCAGCAGCTGAGG
  GGGCCCCAGGAGAGGAAGCAGAGGCGGAGAAGGCCACTGTCCCTGCCGGGGAAGGAGTAAGTTTAGAGGA
  GGCCAAAATTGGAACTGAAACCACTGAGGGTGCAGAGAGTGCCCAACCTGAAGCAGAGGAGCTCGAAGCA
  ACAGTGCCTCAGGAGAAGGTCATTCCTTCGGTGGTCATAGAGCCTGCCTCCAACCATGAAGAGGAAGGAG
  AAAACGAAATAACTATAGGTGCAGAGCCCAAGGAGACCACCGAGGACGCGGCTCCTCCGGGCCCCACCAG
  CGAGACACCGGAGCTGGCTACGGAGCAGAAGCCTATCCAGGACCCTCAGCCCACGCCTTCTGCACCAGCC
  ATGGGGGCTGCTGACCAGCTAGCATCTGCAAGGGAGGCCTCTCAGGAATTGCCTCCTGGCTTTCTCTACA
  AGGTGGAAACACTGCATGATTTTGAGGCAGCAAATTCTGATGAACTTACCTTACAAAGGGGTGATGTGGT
  GCTGGTGGTCCCCTCAGATTCAGAAGCTGATCAGGATGCAGGCTGGCTGGTGGGAGTGAAGGAATCAGAC
  TGGCTTCAGTACAGAGACCTTGCCACCTACAAAGGCCTCTTTCCAGAGAACTTCACCCGACGCTTAGATT
  AG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_139316
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139316.2, NP_647477.1
• RefSeq Size: 3300 bp
• RefSeq ORF: 1962 bp
• Locus ID: 273
• Cytogenetics: 7p14.1
• Domains: SH3, BAR
• Protein Families: Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis
• Gene Summary: 'This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (2) lacks an in-frame exon within the coding region compared to variant 1, resulting in a shorter protein (isoform 2).