alpha Adducin (ADD1) (NM_014190) Human Untagged Clone

CAT#: SC310209

ADD1 (untagged)-Human adducin 1 (alpha) (ADD1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD1
• Synonyms: ADDA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014190, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATGGTGATTCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGT
  ACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGGAGAGGAACATGGCACCAGACCTTCGCCA
  GGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAA
  TTGGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGA
  TTGCAGATTTTATGACCACGAATGTACCAAATGTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTT
  AAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGTATGACAAA
  GGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGC
  TTATCTACAATCATATCACAACCAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCT
  TCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGAGATATAGTAGATCGTGGA
  AGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACG
  TGAAGTGCGTCGTGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCC
  AATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCATGACTACCATGGCATTCTGGTTGATGAA
  GAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGC
  TCGTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGAT
  CCAGGTTCGAACTCTGGCCAGTGCAGGAGGACCAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAA
  GCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCAGATTGGTG
  AGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATA
  CCCTGCTCTGAGAGAGAAGTCTAAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCC
  TTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTCAGAAGCAGCAGCGGGAGA
  AGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAA
  GTCGAAGACTAAGTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTT
  CCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATCCGAGAGCAGAATTTACAGGACA
  TTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATGGACAGGAGCCTCGTCCAGGGAGA
  GCTGGTGACGGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCCACGTCATTGTGAGCACCACGGGC
  CCCAACCCCTTCACCACACTCACAGACCGTGAGCTGGAGGAGTACCGCAGGGAGGTGGAGAGGAAGCAGA
  AGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAGAGTCCTCCAGACCAGCCTGCGGT
  CCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGGAGACGGATGCGCTAGAGAGTACCTGTTA
  CCCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014190
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014190.3, NP_054909.2
• RefSeq Size: 4004 bp
• RefSeq ORF: 1896 bp
• Locus ID: 118
• Cytogenetics: 4p16.3
• Domains: Aldolase_II
• Protein Families: Druggable Genome
• Gene Summary: 'Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (3) includes an additional exon in the 3' coding region, which results in a frameshift, compared to variant 2. The encoded protein (isoform c) contains an early stop codon and shorter C-terminus, compared to isoform b. Variants 3, 9, and 10 all encode the same isoform (c).