OSBPL9 (NM_148906) Human Untagged Clone

CAT#: SC310259

OSBPL9 (untagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OSBPL9
• Synonyms: ORP-9; ORP9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_148906, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGGT
  TCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGGGAGCTGTGATTGGTATAGACGATGAGGA
  CGACAGCACCTTCACAATAACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGA
  GAGAAGTGGATCCATGCCTTAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAGGTTTGGATTCAG
  GATTTGTTCCTAGTGTCCAAGATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGAT
  TGAACAATTAAAGCTTTTTGATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAA
  ACTCTCAAAGAGACAACAAATAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCA
  AAGACCAGAGTAATGCGGAGAAGCACGCAGATGGAATGATAAGTACTATTAATCCCGTAGATGCAATATA
  TCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCTCCAGAACCTGTT
  CAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTACCTTGGGACATC
  ATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCTCACTTCTCCAAG
  CCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATGAATTTTATGAT
  GCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGATCTGCCTCAGTCC
  TGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAATTCTTCCTTGTC
  CAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGACAGAGATGATGATGCGGAGGCAGGGTCT
  GTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGGATCTTACTAAGG
  TAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTTTTTTGCACATCC
  GGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTTGTGAAATGGTAC
  CTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCATTTTGGGCGAGA
  TTTTTCAGTGTCATTGGACATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTCAGAAGGACCAGT
  TCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCACCCATTTCAGCC
  TTTTATGCTGAGTGTTTTAACAAGAAGATACAATTCAATGCTCATATCTGGACCAAATCAAAATTCCTTG
  GGATGTCAATTGGGGTGCACAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGATGAACATTACAT
  TCTCACATTCCCCAATGGCTATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTAGGAGGAGAATGC
  AATATTAATTGTTCCAAAACAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCTTCTATGGGGGCA
  AGAAGCACAGAATTACTGCCGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTCAATTGAAGGGGA
  ATGGAATGGTGTGATGTATGCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGATACCAAGAAGTTG
  CCTATAATCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCAGCCTTTGGAAGG
  ATGTCACTTTCAACTTAAAAATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAGGCTTGAAGAAAG
  ACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTATTTCATGAAGAT
  GGAGAATGCTGGGTTTATGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_148906
• ORF Size: 2160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_148906.2, NP_683704.2
• RefSeq Size: 3844
• RefSeq ORF: 2160
• Locus ID: 114883
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, uses an alternate start codon, and includes an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) has a longer and distinct N-terminus, compared to isoform a. Isoform b contains both a pleckstrin homology domain and a sterol-binding domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. This transcript contains a uAUG that would result in an 18 aa N-terminal extension, but it has a weak Kozak signal, is not supported by the vast majority of human and homologous transcripts, and dAUG use is supported by data in PMID:19413330, which selectively purifies acetylated protein N-termini and shows that Ala-2 is acetylated following cleavage of Met-1 as encoded by the dAUG.