ERCC8 (NM_000082) Human Untagged Clone

CAT#: SC310441

ERCC8 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC8
• Synonyms: CKN1; CSA; UVSS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000082, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGGGTTTTTGTCCGCACGCCAAACGGGTTTGGAGGACCCTCTTCGCCTTCGGAGAGCAGAGTCAA
  CACGGAGAGTTTTGGGACTGGAATTAAATAAAGACAGAGATGTTGAAAGAATCCACGGCGGTGGAATTAA
  CACCCTTGACATTGAACCTGTTGAAGGGAGATACATGTTATCAGGTGGTTCAGATGGTGTGATTGTACTT
  TATGACCTTGAGAACTCCAGCAGACAATCTTATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAGATC
  ATCCTGATGTTCACAGATACAGTGTGGAGACTGTACAGTGGTATCCTCATGACACTGGCATGTTCACATC
  AAGCTCATTTGATAAAACTCTGAAAGTATGGGATACAAATACATTACAAACTGCAGATGTATTTAATTTT
  GAGGAAACAGTTTATAGTCATCATATGTCTCCAGTCTCCACCAAGCACTGTTTGGTAGCAGTTGGTACTA
  GAGGACCCAAAGTACAACTTTGTGACTTGAAGTCTGGATCCTGTTCTCACATTCTACAGGGTCACAGACA
  AGAAATATTAGCAGTTTCCTGGTCTCCACGTTATGACTATATCTTGGCAACAGCAAGTGCTGACAGTAGA
  GTAAAATTATGGGATGTGAGAAGAGCATCAGGATGTTTGATTACTCTTGATCAACATAATGGGAAAAAGT
  CACAAGCTGTTGAATCAGCAAACACTGCTCATAATGGGAAAGTTAATGGCTTATGTTTTACAAGTGATGG
  ACTTCACCTCCTCACTGTTGGTACAGATAATCGAATGAGGCTCTGGAATAGTTCCAATGGAGAAAACACA
  CTTGTGAACTATGGAAAAGTTTGTAATAACAGTAAAAAAGGATTGAAATTCACTGTCTCCTGTGGCTGCA
  GTTCAGAATTTGTTTTTGTACCATATGGTAGCACCATTGCTGTTTATACAGTTTACTCAGGAGAACAGAT
  AACTATGCTTAAGGGACATTATAAAACTGTTGACTGCTGTGTATTTCAGTCAAATTTCCAGGAACTTTAT
  AGTGGTAGCAGAGACTGCAACATTCTGGCTTGGGTTCCATCCTTATATGAACCAGTTCCTGATGATGATG
  AGACTACAACAAAATCACAATTAAATCCGGCCTTTGAAGATGCCTGGAGCAGCAGTGATGAAGAAGGATG
  A
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000082
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000082.3, NP_000073.1
• RefSeq Size: 2044 bp
• RefSeq ORF: 1191 bp
• Locus ID: 1161
• Cytogenetics: 5q12.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).