GLEPP1 (PTPRO) (NM_030668) Human Untagged Clone

CAT#: SC313211

PTPRO (untagged)-Human protein tyrosine phosphatase, receptor type, O (PTPRO), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRO
• Synonyms: GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT; PTPU2; R-PTP-O
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030668, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTTACAGAGATGAATCCCAATGTGGTAGTGATCTCCGTGCTGGCCATCCTTAGCACACTTTTAATTG
  GACTGTTGCTTGTTACCCTCATTATTCTTAGGAAAAAGCATCTGCAGATGGCTAGGGAGTGTGGAGCTGG
  TACATTTGTCAATTTTGCATCCTTAGAGAGGGATGGAAAGCTTCCATACAACTGGAGTAAAAATGGTTTA
  AAGAAGAGGAAACTGACAAACCCGGTTCAACTGGATGACTTTGATGCCTATATTAAGGATATGGCCAAAG
  ACTCTGACTATAAATTTTCTCTTCAGTTTGAGGAGTTGAAATTGATTGGACTGGATATCCCACACTTTGC
  TGCAGATCTTCCACTGAATCGATGTAAAAACCGTTACACAAACATCCTACCATATGACTTCAGCCGTGTG
  AGATTAGTCTCCATGAATGAAGAGGAAGGTGCAGACTACATCAATGCCAACTATATTCCTGGATACAACT
  CACCCCAGGAGTATATTGCCACCCAGGGGCCACTGCCTGAAACCAGAAATGACTTCTGGAAGATGGTCCT
  GCAACAAAAGTCTCAGATTATTGTCATGCTCACTCAGTGTAATGAGAAAAGGAGGGTGAAATGTGACCAT
  TACTGGCCATTCACGGAAGAACCTATAGCCTATGGAGACATCACTGTGGAGATGATTTCAGAGGAAGAGC
  AGGACGACTGGGCCTGTAGACACTTCCGGATCAACTATGCTGACGAGATGCAGGATGTGATGCATTTTAA
  CTACACTGCATGGCCTGATCATGGTGTGCCCACAGCAAATGCTGCAGAAAGTATCCTGCAGTTTGTACAC
  ATGGTCCGACAGCAAGCTACCAAGAGCAAAGGTCCCATGATCATTCACTGCAGTGCTGGCGTGGGACGGA
  CAGGAACATTCATTGCCCTGGACAGGCTCTTGCAGCACATTCGGGATCATGAGTTTGTTGACATCTTAGG
  GCTGGTGTCAGAAATGAGGTCATACCGGATGTCTATGGTACAGACAGAGGAGCAGTACATTTTTATCCAT
  CAGTGTGTGCAACTGATGTGGATGAAGAAGAAGCAGCAGTTCTGCATCAGTGATGTCATATACGAGAATG
  TTAGCAAGTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_030668
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030668.2, NP_109593.1
• RefSeq Size: 3886 bp
• RefSeq ORF: 1134 bp
• Locus ID: 5800
• Cytogenetics: 12p13-p12
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Phosphatase, Transmembrane
• Gene Summary: 'This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters, and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (4) differs in the 5' UTR and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. Variants 4 and 6 encode the same isoform (d), which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.