Cortactin (CTTN) (NM_005231) Human Untagged Clone

CAT#: SC313961

CTTN (untagged)-Human cortactin (CTTN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTTN
• Synonyms: EMS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005231, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATGACTGGGAGA
  CCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGCTC
  CGGGCACCAGGAGCATATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTTAAG
  GAGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAAGACC
  GAATGGATAAGTCAGCTGTCGGCCACGAATATCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTGGACTC
  GGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATCAGTCTGCTGTAGGCTTTGAA
  TACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCCAGTGGTTTTGGCGGCAAGTATGGCG
  TGCAGGCCGACCGAGTAGACAAGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGAAGCACGAGTC
  ACAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGTATCGACAAGGACAAAGTGGATAAGAGCGCC
  GTTGGCTTTGAGTATCAAGGCAAAACGGAGAAGCACGAGTCCCAGAAAGACTATGTGAAAGGGTTTGGAG
  GAAAATTTGGTGTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGGGATCACCAGGAGAAATTGCA
  GCTGCATGAATCCCAAAAAGATTATAAGACTGGTTTTGGAGGCAAATTCGGTGTTCAGTCGGAGAGGCAG
  GACTCCGCTGCTGTGGGGTTTGATTACAAGGAGAAGCTGGCCAAGCACGAGTCCCAGCAAGACTACTCCA
  AAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATGGATAAGAATGCGTCAACCTTTGAGGATGT
  CACCCAGGTGTCCTCTGCCTACCAGAAGACAGTACCTGTCGAAGCTGTGACCAGCAAAACAAGTAACATC
  AGAGCTAACTTTGAAAACCTCGCTAAGGAGAAAGAGCAGGAGGACAGGCGGAAGGCGGAGGCGGAGAGAG
  CCCAGCGGATGGCCAAGGAGCGGCAGGAGCAGGAAGAGGCCAGGAGGAAGCTGGAGGAGCAAGCCAGAGC
  CAAAACGCAAACGCCCCCTGTGTCGCCCGCACCTCAGCCAACCGAGGAGAGGCTGCCCTCGAGCCCCGTC
  TATGAGGATGCGGCTTCCTTCAAGGCAGAGCTGAGCTACAGAGGCCCTGTGAGTGGGACGGAGCCGGAGC
  CCGTGTACAGCATGGAGGCCGCTGACTACCGAGAGGCCAGCAGCCAGCAGGGCCTGGCCTATGCCACAGA
  GGCTGTCTATGAAAGCGCAGAGGCCCCGGGCCACTATCCCGCAGAGGACAGCACCTACGATGAGTACGAG
  AACGATCTGGGGATCACAGCCGTCGCCCTGTACGACTACCAGGCTGCGGGCGATGATGAGATCTCATTTG
  ACCCTGATGACATCATCACCAACATCGAGATGATTGACGACGGCTGGTGGCGCGGGGTGTGCAAGGGCCG
  GTACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005231
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005231.3, NP_005222.2
• RefSeq Size: 3319 bp
• RefSeq ORF: 1653 bp
• Locus ID: 2017
• Cytogenetics: 11q13.3
• Domains: SH3, HS1_rep
• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• Gene Summary: 'This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) is the longest transcript.