IMPDH1 (NM_000883) Human Untagged Clone

CAT#: SC314151

IMPDH1 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IMPDH1
• Synonyms: IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000883 edited
  ATGGAGGGGCCACTCACTCCACCACCGCTGCAGGGAGGCGGAGCCGCCGCTGTTCCGGAG
  CCCGGAGCCCGGCAACACCCGGGACACGAGACGGCGGCGCAGCGGTACAGCGCCCGACTG
  CTGCAGGCCGGCTACGAGCCCGAGAGCCCTAGATTGGACCTCGCTACACACCCGACGACA
  CCCCGTTCAGAACTATCTTCAGTGGTCTTACTGGCAGGTGTTGGTGTCCAGATGGATCGC
  CTTCGCAGGGCTAGCATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTGCCCGAG
  GATGGGCTCACCGCGCAGCAGCTCTTCGCCAGCGCCGACGGCCTCACCTACAACGACTTC
  CTGATTCTCCCAGGATTCATAGACTTCATAGCTGATGAGGTGGACCTGACCTCAGCCCTG
  ACCCGGAAGATCACGCTGAAGACGCCACTGATCTCCTCCCCCATGGACACTGTGACAGAG
  GCTGACATGGCCATTGCCATGGCTCTGATGGGAGGTATTGGTTTCATTCACCACAACTGC
  ACCCCAGAGTTCCAGGCCAACGAGGTGCGGAAGGTCAAGAAGTTTGAACAGGGCTTCATC
  ACGGACCCTGTGGTGCTGAGCCCCTCGCACACTGTGGGCGATGTGCTGGAGGCCAAGATG
  CGGCATGGCTTCTCTGGCATCCCCATCACTGAGACGGGCACCATGGGCAGCAAGCTGGTG
  GGCATCGTCACCTCCCGAGACATCGACTTTCTTGCTGAGAAGGACCACACCACCCTCCTC
  AGTGAGGTGATGACGCCAAGGATTGAACTGGTGGTGGCTCCAGCAGGTGTGACGTTGAAA
  GAGGCAAATGAGATCCTGCAGCGTAGCAAGAAAGGGAAGCTGCCTATCGTCAATGATTGC
  GATGAGCTGGTGGCCATCATCGCCCGCACCGACCTGAAGAAGAACCGAGACTACCCTCTG
  GCCTCCAAGGATTCCCAGAAGCAGCTGCTCTGTGGGGCAGCTGTGGGCACCCGTGAGGAT
  GACAAATACCGTCTGGACCTGCTCACCCAGGCGGGCGTCGACGTCATAGTCTTGGACTCG
  TCCCAAGGGAATTCGGTGTATCAGATCGCCATGGTGCATTACATCAAACAGAAGTACCCC
  CACCTCCAGGTGATTGGGGGGAACGTGGTGACAGCAGCCCAGGCCAAGAACCTGATTGAT
  GCTGGTGTGGACGGGCTGCGCGTGGGCATGGGCTGCGGCTCCATCTGCATCACCCAGGAA
  GTGATGGCCTGTGGTCGGCCCCAGGGCACTGCTGTGTACAAGGTGGCTGAGTATGCCCGG
  CGCTTTGGTGTGCCCATCATAGCCGATGGCGGCATCCAGACCGTGGGACACGTGGTCAAG
  GCCCTGGCCCTTGGAGCCTCCACAGTGATGATGGGCTCCCTGCTGGCCGCCACTACGGAG
  GCCCCTGGCGAGTACTTCTTCTCAGACGGGGTGCGGCTCAAGAAGTACCGGGGCATGGGC
  TCACTGGATGCCATGGAGAAGAGCAGCAGCAGCCAGAAACGATACTTCAGCGAGGGGGAT
  AAAGTGAAGATCGCRCAGGGTGTCTCGGGCTCCATCCAGGACAAAGGATCCATTCAGAAG
  TTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATATCGGGGCCCGCAGC
  CTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATG
  TCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAAAGCGGCTGTACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000883 unedited
  TCGTACATTCGGAGTTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATA
  TCGGGGCCCGCAGCCTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTG
  AGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAA
  AGCGGCTGTACTGAGGACAGCGGTGGAGGCCGAGGTGGTGGAGGGGATGCACCCCAGTGT
  CCACTTTTGGGCACAGCCTCCCTCCATAACTGAGTGGTCCACAGATTTGCACTACGGGTT
  CTCCAGCTCCTTTCCAGGCAGAGAGGAGGGGAGGTCCTGAGGGGACTGCTGCCCCTCACT
  CGGCATCCCCTGCAGAGTCAGGACTGCTCCCGGGGCCAGGCTGCCCTGGGAGCCCCCCTC
  CGAGCCCAGCCAGCCAGGCTCTCAGGCCCTGCGCCTGCCTCAGGTCTTTCTTGCTGCAGC
  CTGCTCCAGCCTGGCCCCCACCCCAGGGGCAGGCGGCCCCTCCTGGCTTCTCCTGTAGGG
  CACCTCCCTGCCCCTAGCCTCCCAGGAAATGGTGCTCTCCTGGCCCTGCCTCTGGCCCTT
  CCCGGGCCGCTGCCCCTCAGCCATGTGGCACTTCTGAGCTCCTGACCTAGCCAAGGGGAG
  GTCTCTGCCCCCTTCCCCGGCCCTGGGCTACCCTTGGGTCCTGCTCCTCATGCCGCTCCC
  CTGTCCCTGGCCATGGGTAGGAGACTGCCCTGGTCATGGCCCGCCTGCCTGTCATTCCTG
  ACTCACCACCGTCCCCAGGTGAACCATTCCTCCCTTCTCCTCAGCTGCAGTCGAAGCTTT
  AACTTTGCACACTTGGATCACAGTTGCGTCATTGTGTATTAAAATACTTGGAATAAATCA
  ACCACGTTCTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_000883
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000883.2, NP_000874.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 1800 bp
• Locus ID: 3614
• Cytogenetics: 7q32.1
• Domains: CBS, IMPDH
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: 'The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).