GIT2 (NM_057170) Human Untagged Clone

CAT#: SC314271

GIT2 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GIT2
• Synonyms: CAT-2; CAT2; PKL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_057170, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAG
  TAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCCCA
  AGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATAAC
  GGTGCTAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTA
  ATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGT
  CCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC
  GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATC
  CTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATTTTACAGGCTGAATTATTGGC
  AGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCAAGGCAAGGA
  GGGCACCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATC
  TCTGTGGCAGGAAACCAGATCACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGCAGCCT
  GGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTTGAAGAA
  CTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTTGCCACGCAAAACC
  ACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAGTACTCATCAACACG
  AAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCCACGCTGGTCATTGACATTCTC
  AGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGA
  AAACCATCAATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATC
  AGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGAGCCTAGATTCAGATTTA
  TCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTGGCTTCTGAGGCCAAGA
  TACAGCAGCTAATGAAGGCATCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAGTGACTACGACAACAC
  TCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGGACGGCAAAGAAGTATGGTGTGGCCA
  GGGGATGGCTTGGTACCAGACACAGCAGAACCCCATGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAG
  ATGTCATCAGGAAGACTGAACAGATCACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAA
  ACATGACAGTTATATTCCCTGCTCAGAGAGGATACACGTAGCTGTTACAGAAATGGCAGCATTATTCCCC
  AAAAAACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGACTGCAGT
  CAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGCTGGTCACGCAGCAGGT
  CATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGGTTACCATCACCACCAAAGAGAACAAC
  AACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_057170
• ORF Size: 1896 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_057170.3, NP_476511.1
• RefSeq Size: 5259
• RefSeq ORF: 1896
• Locus ID: 9815
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (2) lacks two exons and a portion of a third exon in the coding region, as compared to variant 1. The reading frame is maintained. Isoform 2 is missing internal 128 aa but has the same N- and C-termini as isoform 1.