Synapsin I (SYN1) (NM_133499) Human Untagged Clone

CAT#: SC314376

SYN1 (untagged)-Human synapsin I (SYN1), transcript variant Ib

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SYN1
• Synonyms: SYN1a; SYN1b; SYNI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133499, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACTACCTGCGGCGCCGCCTGTCGGACAGCAACTTTATGGCCAATCTGCCAAATGGGTACATGACAG
  ACCTGCAGCGTCCGCAGCCGCCCCCACCGCCGCCCGGTGCCCACAGCCCCGGAGCCACGCCCGGTCCCGG
  GACCGCCACTGCCGAGAGGTCCTCCGGGGTCGCCCCAGCGGCCTCTCCGGCCGCCCCTAGCCCCGGGTCC
  TCGGGGGGCGGTGGCTTCTTCTCGTCGCTGTCCAACGCGGTCAAGCAGACCACGGCGGCGGCAGCTGCCA
  CCTTCAGCGAGCAGGTGGGCGGCGGCTCTGGGGGCGCAGGCCGCGGGGGAGCCGCCTCCAGGGTGCTGCT
  GGTCATCGACGAGCCGCACACCGACTGGGCAAAATACTTCAAAGGGAAAAAGATCCATGGAGAAATTGAC
  ATTAAAGTAGAACAGGCCGAATTCTCTGATCTCAACCTTGTGGCCCATGCCAATGGTGGATTCTCTGTGG
  ATATGGAAGTTCTTCGGAATGGGGTGAAGGTCGTGCGGTCTCTGAAGCCGGATTTTGTGCTGATCCGCCA
  GCACGCCTTCAGCATGGCACGCAACGGAGACTACCGCAGTTTGGTCATTGGGCTGCAGTATGCTGGAATC
  CCCAGTGTTAACTCCTTGCATTCTGTCTACAACTTCTGTGACAAGCCCTGGGTGTTTGCCCAGATGGTTC
  GACTGCATAAGAAACTGGGGACAGAAGAATTCCCTCTAATTGATCAGACCTTCTACCCCAATCACAAAGA
  AATGCTCAGCAGTACAACGTACCCCGTGGTTGTGAAGATGGGGCACGCACACTCTGGGATGGGCAAGGTC
  AAGGTTGACAACCAGCATGACTTCCAGGACATCGCAAGTGTCGTGGCACTGACCAAGACGTATGCCACTG
  CCGAGCCCTTCATCGATGCCAAATATGACGTGCGTGTCCAGAAGATTGGGCAGAACTACAAGGCCTACAT
  GAGGACGTCAGTGTCAGGGAACTGGAAGACCAATACTGGCTCTGCGATGCTGGAGCAAATTGCCATGTCT
  GACAGATACAAGCTGTGGGTGGACACGTGCTCAGAGATTTTTGGGGGACTGGACATCTGCGCAGTGGAAG
  CGCTACATGGCAAGGACGGAAGGGATCACATCATTGAGGTGGTGGGTTCCTCCATGCCGCTCATTGGTGA
  CCACCAGGATGAAGACAAACAGCTCATCGTAGAGCTCGTGGTCAACAAGATGGCTCAGGCCCTGCCCCGG
  CAGCGACAGCGGGATGCCTCCCCTGGCAGGGGCTCCCATGGCCAGACTCCGTCCCCAGGGGCCCTGCCCT
  TGGGCCGCCAGACCTCCCAGCAGCCCGCAGGGCCCCCGGCTCAGCAGCGACCCCCACCACAGGGCGGCCC
  TCCACAGCCGGGTCCAGGCCCCCAGCGCCAGGGACCCCCATTGCAGCAGCGCCCGCCCCCGCAGGGCCAG
  CAGCACCTTTCAGGCCTTGGACCCCCAGCTGGCAGCCCCCTGCCCCAGCGCCTTCCAAGTCCCACCTCAG
  CGCCCCAGCAGCCCGCGTCCCAGGCCGCGCCGCCGACCCAGGGTCAAGGCCGCCAATCCCGGCCAGTGGC
  GGGAGGCCCCGGGGCGCCTCCAGCAGCCCGCCCGCCCGCCTCTCCGTCTCCCCAGCGCCAGGCGGGCCCC
  CCACAGGCTACCCGTCAGACATCCGTCTCTGGCCCGGCTCCGCCAAAGGCCTCTGGGGCCCCACCGGGCG
  GGCAGCAGCGCCAGGGCCCGCCCCAGAAACCCCCAGGCCCAGCCGGCCCCACACGCCAGGCCAGCCAGGC
  GGGTCCCGTGCCCCGCACTGGGCCACCCACCACGCAGCAGCCTCGGCCCAGCGGCCCGGGCCCCGCTGGA
  CGTCCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGACGTGCCGCCACCCGCCACCGCCGCTGCAGGGG
  GACCTCCGCACCCCCAGCTCAAAGCCAGCCCCGCCCAGGCCCAGCCTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_133499
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133499.2, NP_598006.1
• RefSeq Size: 3172 bp
• RefSeq ORF: 2010 bp
• Locus ID: 6853
• Cytogenetics: Xp11.3-p11.23
• Gene Summary: 'This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (Ib) utilizes an alternative splice acceptor site in terminal exon compared to variant Ia, resulting in the lack of an internal segment and a frameshift. This variant encodes isoform Ib, which contains a shorter carboxyl terminus and a distinct domain F, as compared to isoform Ia.