RFX2 (NM_134433) Human Untagged Clone

CAT#: SC314460

RFX2 (untagged)-Human regulatory factor X, 2 (influences HLA class II expression) (RFX2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX2
• Synonyms: FLJ14226
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_134433, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGAATTCCGAGGGTGGAGCGGATTCGCCAGCGTCCGTGGCTCTGCGTCCCTCGGCGGCAGCCCCGC
  CTGTGCCAGCCTCCCCGCAGAGGGTGTTGGTCCAGGCAGCCAGCTCCAATCCCAAAGGGGCCCAGATGCA
  GCCGATCTCCCTCCCCAGAGTTCAGCAGGTACCCCAGCAGGTGCAGCCGGTGCAGCACGTGTATCCTGCC
  CAGGTGCAGTACGTGGAAGGGGGAGACGCCGTCTACACCAATGGAGCCATACGAACAGCCTACACCTACA
  ACCCCGAGCCTCAGATGTACGCCCCCAGCAGCACGGCTTCTTACTTCGAGGCCCCAGGCGGTGCCCAGGT
  GACCGTGGCAGCCTCGTCCCCGCCAGCGGTCCCCTCCCACAGCATGGTGGGCATCACCATGGATGTCGGG
  GGGAGCCCCATCGTCTCCAGCGCGGGAGCCTATCTCATCCACGGGGGGATGGACAGCACCAGACACTCCC
  TGGCCCACACCTCCCGCTCATCGCCCGCCACGCTCCAGTGGCTGTTGGATAATTATGAAACAGCGGAAGG
  TGTGAGTCTCCCCAGAAGTTCTCTTTACAACCACTACCTTCGGCACTGCCAGGAGCACAAGCTAGACCCA
  GTGAACGCCGCCTCCTTCGGGAAACTGATCCGTTCTGTGTTTATGGGGCTGAGAACGCGGCGGCTGGGCA
  CCAGGGGCAACTCGAAGTACCATTACTATGGGATTCGTCTGAAGCCGGACTCACCACTGAACCGGCTGCA
  GGAGGACACGCAGTACATGGCCATGCGGCAGCAGCCCATGCACCAGAAGCCCAGGTACCGGCCAGCCCAG
  AAGACGGACAGCCTCGGGGACAGCGGCTCCCACAGCGGCCTGCACAGCACTCCGGAACAGACCATGGCCG
  TGCAGAGCCAGCACCACCAGCAGTACATAGATGTCTCCCACGTCTTCCCCGAGTTCCCAGCGCCCGACCT
  GGGCAGCTTCCTGCTGCAGGACGGCGTCACACTGCACGACGTCAAGGCCCTGCAGCTGGTGTACAGACGG
  CACTGCGAGGCAACTGTAGATGTGGTGATGAACCTCCAGTTCCACTACATCGAGAAGCTGTGGCTCTCCT
  TCTGGAACTCTAAGGCCTCCTCCAGCGACGGCCCCACCTCTCTTCCTGCCAGTGACGAAGACCCCGAGGG
  CGCCGTCCTGCCCAAGGACAAGCTTATCTCCCTGTGTCAGTGCGACCCCATCCTCAGGTGGATGAGGAGC
  TGCGACCACATCCTCTACCAGGCGCTGGTGGAGATTCTCATCCCCGACGTGCTGAGGCCGGTCCCCAGTA
  CCTTGACACAGGCCATCCGTAACTTTGCCAAGAGCTTGGAAGGCTGGTTGACAAATGCCATGAGTGACTT
  CCCACAACAGGTCATCCAGACCAAGGTGGGCGTCGTCAGTGCCTTCGCCCAGACGCTGCGGCGCTACACG
  TCCCTCAACCACCTGGCGCAGGCGGCCCGGGCGGTGCTGCAGAACACGTCCCAGATCAACCAGATGCTCA
  GCGACCTCAACCGCGTGGACTTTGCCAACGTGCAGGAGCAGGCCTCGTGGGTGTGCCAGTGCGAGGAGAG
  TGTGGTGCAGCGGCTGGAGCAGGATTTCAAGCTGACCCTGCAGCAGCAGAGCTCCCTGGACCAGTGGGCC
  AGCTGGCTGGACAGTGTGGTCACCCAGGTCCTGAAGCAGCATGCCGGCAGCCCCAGCTTCCCCAAGGCCG
  CCCGGCAGTTCTTGCTGAAATGGTCCTTTTACAGCTCCATGGTGATCCGGGACCTGACCCTGCGCAGCGC
  TGCCAGCTTCGGCTCCTTCCACCTCATCCGCCTGCTCTACGACGAGTACATGTTCTACCTGGTGGAGCAC
  CGCGTCGCGGAGGCCACCGGAGAGACGCCGATCGCTGTGATGGGAGAGTTCAACGATCTCGCCTCTCTGT
  CGCTGACGCTGCTCGACAAAGATGACATGGGCGATGAGCAGCGTGGCAGCGAGGCGGGCCCAGACGCCCG
  CAGCCTGGGTGAGCCCCTGGTAAAGCGGGAGCGCAGTGACCCCAACCACTCCCTGCAGGGCATCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_134433
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134433.2, NP_602309.1
• RefSeq Size: 4039 bp
• RefSeq ORF: 2097 bp
• Locus ID: 5990
• Cytogenetics: 19p13.3
• Domains: RFX_DNA_binding, RFX1_trans_act
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) includes an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) when it is compared to isoform a.