OSBPL9 (NM_148909) Human Untagged Clone

CAT#: SC314600

OSBPL9 (untagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OSBPL9
• Synonyms: ORP-9; ORP9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_148909, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGGT
  TCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAAAATGATGAGAGGCTCTCG
  CAGAGGATGTGTTAGACTCAGAGGAGCTGTGATTGGTATAGACGATGAGGACGACAGCACCTTCACAATA
  ACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGAGAGAAGTGGATCCATGCCT
  TAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAATCAGTACCACACTTGCTTTTTTCCAGTCTTC
  TGGTATCTCTCCAGTTCTTGAATTTTCAAAAATAATTGGTTTGGATTCAGGATTTGTTCCTAGTGTCCAA
  GATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGATTGAACAATTAAAGCTTTTTG
  ATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAGACAACAAA
  TAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATTAATCCCGTA
  GATGCAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCTC
  CAGAACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTAC
  CTTGGGACATCATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCTC
  ACTTCTCCAAGCCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATG
  AATTTTATGATGCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGATC
  TGCCTCAGTCCTGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAAT
  TCTTCCTTGTCCAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGACAGAGATGATGATGCGG
  AGGCAGGGTCTGTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGGA
  TCTTACTAAGGTAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTTT
  TTTGCACATCCGGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTTG
  TGAAATGGTACCTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCAT
  TTTGGGCGAGATTTTTCAGTGTCATTGGACATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTCA
  GAAGGACCAGTTCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCAC
  CCATTTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACAATTCAATGCTCATATCTGGACCAAATC
  AAAATTCCTTGGGATGTCAATTGGGGTGCACAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGAT
  GAACATTACATTCTCACATTCCCCAATGGCTATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTAG
  GAGGAGAATGCAATATTAATTGTTCCAAAACAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCTT
  CTATGGGGGCAAGAAGCACAGAATTACTGCCGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTCA
  ATTGAAGGGGAATGGAATGGTGTGATGTATGCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGATA
  CCAAGAAGTTGCCTATAATCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCAG
  CCTTTGGAAGGATGTCACTTTCAACTTAAAAATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAGG
  CTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTAT
  TTCATGAAGATGGAGAATGCTGGGTTTATGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCATTA
  G
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_148909
• ORF Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_148909.3, NP_683707.3
• RefSeq Size: 3925
• RefSeq ORF: 2241
• Locus ID: 114883
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (7) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (f) has a longer and distinct N-terminus, compared to isoform a. Isoform f contains both a pleckstrin homology domain and a sterol-binding domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. This transcript contains a uAUG that would result in an 18 aa N-terminal extension, but it has a weak Kozak signal, is not supported by the vast majority of human and homologous transcripts, and dAUG use is supported by data in PMID:19413330, which selectively purifies acetylated protein N-termini and shows that Ala-2 is acetylated following cleavage of Met-1 as encoded by the dAUG. CCDS Note: This CCDS representation uses a downstream start codon but includes a potential upstream start codon that would result in a protein that is 18 aa longer at the N-terminus. However, the upstream start codon has a weak Kozak signal, the vast majority of transcripts from human and homologous species start downstream of it, and data in PMID:19413330 indicate that the N-terminus begins at an N-alpha acetylated Ala-2 residue (nASIMEGPLSK...) following cleavage of the initiating Met-1 derived from the second start codon. The second start codon is therefore represented in this CCDS.