BRDT (NM_001726) Human Untagged Clone

CAT#: SC314970

BRDT (untagged)-Human bromodomain, testis-specific (BRDT), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRDT
• Synonyms: BRD6; CT9; SPGF21
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001726, the custom clone sequence may differ by one or more nucleotides
  ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATA
  AATACTAAGAAAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTA
  AAGGATTTATGGAAGCATAGTTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAA
  CTACAGTTGCCTGATTATTATACCATTATAAAAAACCCAATGGATTTAAATACAATTAAG
  AAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCAGAATGTATAGAAGACTTCAATACA
  ATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATTGTTCTTATGGCACAA
  GCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTG
  GGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAA
  GAAAAATCATCACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTA
  TTTCCTAAGACATCTATTTCTCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGT
  TCACAAACTGCGGCCCAAGTTACAAAAGGTGTGAAGAGGAAAGCAGATACAACAACTCCT
  GCAACTTCAGCAGTTAAAGCAAGTAGTGAATTTTCTCCAACATTCACAGAAAAATCAGTG
  GCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTTTTGCCAGATTCTCAGCAACAA
  TATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGTAGTGAGATTCTT
  AAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTTGAC
  GTTAATGCTTTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGA
  ACTATTAAGGAGAAAATGGATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGAT
  GTTAGATTAATGTTCATGAATTGCTACAAGTACAATCCTCCAGATCACGAAGTTGTGACA
  ATGGCAAGAATGCTTCAGGATGTTTTCGAAACGCATTTTTCAAAGATCCCGATTGAACCT
  GTTGAGAGTATGCCTTTATGTTACATCAAAACAGATATCACAGAAACCACTGGTAGAGAG
  AACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGATTCTGAAGATGAGCGAGTT
  AAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTCCAGGTTTTG
  TCCCAAGTACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAA
  AAAGAAAAGGTTAATAACAGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTA
  AAGGAAAAGTCCAAGAGAAATCAGCCAAAGAAAAGGAAACAACAGTTCATTGGTCTAAAA
  TCTGAAGATGAAGATAATGCTAAACCTATGAACTATGATGAGAAAAGGCAGTTAAGTCTG
  AATATAAACAAACTCCCTGGAGATAAACTTGGGCGAGTAGTTCACATAATACAATCAAGA
  GAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATAGAGATAGACTTTGAAACACTGAAA
  GCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTAAGAAAGAGACCATTA
  AAACCTCCTGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAAAAACAG
  GAATTGGAAAAGCGGTTACTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACA
  AAATCTGATAAAACGCAACCATCCAAAGCTGTTGAAAATGTTTCCCGACTGAGTGAGAGC
  AGCAGCAGCAGCAGCAGCTCATCAGAGTCTGAAAGTAGCAGCAGTGACTTAAGCTCTTCA
  GACAGCAGTGATTCTGAATCAGAAATGTTCCCTAAGTTTACAGAAGTAAAACCAAATGAT
  TCTCCTTCTAAAGAGAATGTAAAGAAAATGAAGAATGAATGCATACTGCCTGAAGGAAGA
  ACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACAACCTCTGCCAATACTACCCTT
  GTTCATCAGACCACACCTTCACATGTAATGCCACCAAATCACCACCAATTAGCATTTAAT
  TATCAAGAATTAGAACATTTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTGCCT
  CCCTCAGGTGATTCTGAACAGCTCTCAAATGGCATAACTGTGATGCATCCATCTGGTGAT
  AGTGACACAACGATGTTAGAATCTGAATGTCAAGCTCCTGTACAGAAGGATATAAAGATT
  AAGAATGCAGATTCATGGAAAAGTTTAGGCAAACCAGTGAAACCATCAGGTGTAATGAAA
  TCCTCAGATGAGCTCTTCAACCAATTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAGCT
  CGGACACAGGAACTCATACGGAAGCATTTGGAACAAAATACAAAGGAACTAAAAGCATCT
  CAAGAAAATCAGAGGGATCTTGGGAATGGATTGACTGTAGAATCTTTTTCAAATAAAATA
  CAAAACAAGTGCTCTGGAGAAGAGCAGAAAGAACATCAGCAGTCATCAGAAGCTCAAGAT
  AAATCCAAACTCTGGCTTCTCAAAGACCGTGATTTAGCAAGGCAGAAAGAACAAGAGAGG
  AGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGACCCTTCAAAGTGACATTATGACA
  ATGTTTGAAAACAACTTTGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001726
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001726.2, NP_001717.2
• RefSeq Size: 3119 bp
• RefSeq ORF: 2844 bp
• Locus ID: 676
• Cytogenetics: 1p22.1
• Protein Families: Protein Kinase, Transcription Factors
• Gene Summary: 'BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 4. The resulting isoform (b) lacks a short internal segment compared to isoform a. Variants 1, 2, and 3 all encode the same isoform (b).