NBR1 (NM_005899) Human Untagged Clone

CAT#: SC315009

NBR1 (untagged)-Human neighbor of BRCA1 gene 1 (NBR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NBR1
• Synonyms: 1A1-3B; IAI3B; M17S2; MIG19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005899, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAACCACAGGTTACTCTAAATGTGACTTTTAAAAATGAAATTCAAAGCTTTCTGGTTTCTGATCCAG
  AAAATACAACTTGGGCTGATATCGAAGCTATGGTAAAAGTTTCATTTGATCTGAATACTATTCAAATAAA
  ATACCTGGATGAGGAAAATGAAGAGGTATCCATCAACAGTCAAGGAGAATATGAAGAAGCGCTTAAGATG
  GCAGTTAAACAGGGAAACCAACTGCAGATGCAAGTCCACGAAGGGCACCATGTCGTTGATGAAGCCCCAC
  CCCCAGTTGTAGGAGCAAAACGACTAGCTGCCAGGGCAGGGAAGAAGCCACTTGCACATTACTCTTCACT
  GGTGAGAGTCTTGGGATCAGACATGAAGACCCCAGAGGATCCTGCAGTGCAGTCGTTTCCACTTGTTCCA
  TGTGACACAGACCAGCCTCAAGACAAGCCCCCAGACTGGTTCACAAGCTACCTGGAGACGTTCAGAGAAC
  AAGTGGTTAACGAAACGGTTGAGAAGCTTGAACAGAAATTACATGAAAAGCTTGTCCTCCAGAACCCATC
  CTTGGGTTCTTGTCCCTCAGAAGTCTCAATGCCTACTTCAGAAGAAACATTGTTTTTGCCAGAAAACCAG
  TTCAGCTGGCATATTGCTTGCAACAACTGCCAAAGAAGGATTGTTGGTGTCCGCTACCAGTGTAGCCTAT
  GCCCATCCTACAATATCTGTGAAGATTGTGAAGCAGGGCCATATGGCCATGACACTAACCACGTCCTGCT
  GAAGTTGCGGAGACCTGTTGTGGGCTCCTCTGAACCGTTCTGTCACTCAAAGTACTCTACTCCTCGTCTT
  CCTGCTGCTCTGGAACAAGTCAGGCTCCAGAAACAGGTTGATAAGAACTTTCTTAAAGCAGAAAAGCAAA
  GGTTGCGAGCTGAGAAGAAACAACGTAAAGCAGAGGTCAAGGAACTTAAAAAGCAGCTTAAACTCCATAG
  GAAAATTCACCTGTGGAATTCAATCCATGGACTCCAGAGCCCCAAGTCTCCTTTAGGCCGACCTGAGAGC
  TTGCTCCAGTCTAATACCCTGATGCTCCCTTTGCAGCCCTGTACCTCCGTTATGCCAATGCTCAGTGCAG
  CATTTGTGGATGAGAATTTGCCTGATGGGACTCACCTTCAGCCAGGAACCAAGTTTATCAAACACTGGAG
  GATGAAAAATACAGGAAATGTAAAGTGGAGTGCAGACACAAAGCTCAAGTTCATGTGGGGAAACCTGACT
  TTGGCTTCCACAGAAAAGAAGGATGTTTTGGTTCCCTGCCTCAAGGCCGGCCATGTGGGAGTTGTATCTG
  TGGAGTTCATTGCCCCAGCCTTGGAGGGAACGTATACTTCCCATTGGCGTCTTTCTCACAAAGGCCAGCA
  ATTTGGGCCTCGGGTCTGGTGCAGTATCATAGTAGATCCTTTCCCCTCCGAAGAGAGCCCTGATAACATT
  GAAAAGGGCATGATCAGCTCAAGCAAAACTGATGATCTCACCTGCCAGCAAGAGGAAACTTTTCTTCTGG
  CTAAAGAAGAAAGACAGCTTGGTGAAGTGACTGAGCAGACAGAAGGGACAGCAGCCTGCATCCCACAGAA
  GGCAAAAAATGTTGCCAGTGAGAGGGAGCTCTACATCCCATCTGTGGATCTTCTGACTGCCCAGGACCTG
  CTGTCCTTTGAGCTGTTGGATATAAACATTGTTCAAGAGTTGGAGAGAGTGCCCCACAACACCCCTGTGG
  ATGTGACTCCCTGCATGTCTCCTCTGCCACATGACAGTCCTTTAATAGAGAAGCCAGGCTTGGGGCAGAT
  AGAGGAAGAGAATGAAGGGGCAGGATTTAAAGCACTTCCTGATTCTATGGTGTCAGTAAAGAGGAAGGCT
  GAGAACATTGCTTCTGTGGAGGAAGCAGAAGAAGACCTGAGTGGGACCCAGTTTGTGTGTGAGACAGTAA
  TCCGATCCCTTACCTTGGATGCTGCCCCAGACCACAACCCTCCTTGCAGACAGAAGTCCTTGCAGATGAC
  ATTTGCCTTGCCTGAAGGACCACTTGGAAATGAGAAGGAGGAGATTATCCATATCGCTGAGGAAGAAGCT
  GTCATGGAGGAGGAGGAGGATGAGGAGGATGAGGAGGAGGAGGATGAGCTCAAAGATGAAGTTCAAAGTC
  AGTCCTCTGCTTCCTCAGAGGATTACATCATCATCCTGCCTGAGTGCTTTGATACCAGCCGCCCCCTGGG
  GGATTCTATGTACAGCTCTGCGCTCTCACAGCCAGGCCTGGAGCGAGGTGCTGAAGGCAAGCCTGGGGTT
  GAGGCTGGGCAGGAACCAGCTGAGGCTGGGGAAAGACTCCCTGGAGGGGAGAACCAGCCACAGGAGCACA
  GCATAAGTGACATCCTCACGACCTCACAGACTCTGGAAACAGTGCCCCTAATCCCAGAGGTAGTGGAGCT
  TCCACCGTCACTGCCCAGGAGCTCTCCTTGTGTACATCATCATGGTTCCCCAGGAGTGGATTTACCAGTT
  ACCATACCAGAAGTTTCTTCAGTCCCTGATCAGATCAGAGGAGAGCCCAGAGGCTCATCAGGACTTGTAA
  ACAGCAGACAGAAGAGCTATGACCACTCAAGGCACCATCATGGGAGCAGCATTGCTGGAGGACTGGTGAA
  GGGGGCTTTGTCTGTTGCTGCCTCTGCATACAAGGCCCTGTTTGCTGGGCCACCAGTCACTGCACAGCCA
  ATAATTTCTGAAGATCAGACAGCAGCCCTGATGGCCCATCTCTTTGAAATGGGATTCTGTGACAGGCAGC
  TGAACCTACGGCTGCTGAAGAAACACAATTACAATATCCTGCAGGTTGTGACAGAACTTCTTCAGTTAAA
  CAACAACGACTGGTACAGCCAACGCTATTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005899
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005899.4, NP_005890.2
• RefSeq Size: 4874 bp
• RefSeq ORF: 2901 bp
• Locus ID: 4077
• Cytogenetics: 17q21.31
• Domains: PB1, ZnF_ZZ
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 3 encode isoform a.