Polycystin 2 (PKD2) (NM_000297) Human Untagged Clone

CAT#: SC315012

PKD2 (untagged)-Human polycystic kidney disease 2 (autosomal dominant) (PKD2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKD2
• Synonyms: APKD2; Pc-2; PC2; PKD4; TRPP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000297 edited
  GCGCCGGGAAGAAAGGAACATGGCTCCTGAGGCGCACAGCGCCGAGCGCGGCGCCGCGCA
  CCCGCGCGCCGGACGCCAGTGACCGCGATGGTGAACTCCAGTCGCGTGCAGCCTCAGCAG
  CCCGGGGACGCCAAGCGGCCGCCCGCGCCCCGCGCGCCGGACCCGGGCCGGCTGATGGCT
  GGCTGCGCGGCCGTGGGCGCCAGCCTCGCCGCCCCGGGCGGCCTCTGCGAGCAGCGGGGC
  CTGGAGATCGAGATGCAGCGCATCCGGCAGGCGGCCGCGCGGGACCCCCCGGCCGGAGCC
  GCGGCCTCCCCTTCTCCTCCGCTCTCGTCGTGCTCCCGGCAGGCGTGGAGCCGCGATAAC
  CCCGGCTTCGAGGCCGAGGAGGAGGAGGAGGAGGTGGAAGGGGAAGAAGGCGGAATGGTG
  GTGGAGATGGACGTAGAGTGGCGCCCGGGCAGCCGGAGGTCGGCCGCCTCCTCGGCCGTG
  AGCTCCGTGGGCGCGCGGAGCCGGGGGCTTGGGGGCTACCACGGCGCGGGCCACCCGAGC
  GGGAGGCGGCGCCGGCGAGAGGACCAGGGCCCGCCGTGCCCCAGCCCAGTCGGCGGCGGG
  GACCCGCTGCATCGCCACCTCCCCCTGGAAGGGCAGCCGCCCCGAGTGGCCTGGGCGGAG
  AGGCTGGTTCGCGGGCTGCGAGGTCTCTGGGGAACAAGACTCATGGAGGAAAGCAGCACT
  AACCGAGAGAAATACCTTAAAAGTGTTTTACGGGAACTGGTCACATACCTCCTTTTTCTC
  ATAGTCTTGTGCATCTTGACCTACGGCATGATGAGCTCCAATGTGTACTACTACACCCGG
  ATGATGTCACAGCTCTTCCTAGACACCCCCGTGTCCAAAACGGAGAAAACTAACTTTAAA
  ACTCTGTCTTCCATGGAAGACTTCTGGAAGTTCACAGAAGGCTCCTTATTGGATGGGCTG
  TACTGGAAGATGCAGCCCAGCAACCAGACTGAAGCTGACAACCGAAGTTTCATCTTCTAT
  GAGAACCTGCTGTTAGGGGTTCCACGAATACGGCAACTCCGAGTCAGAAATGGATCCTGC
  TCTATCCCCCAGGACTTGAGAGATGAAATTAAAGAGTGCTATGATGTCTACTCTGTCAGT
  AGTGAAGATAGGGCTCCCTTTGGGCCCCGAAATGGAACCGCTTGGATCTACACAAGTGAA
  AAAGACTTGAATGGTAGTAGCCACTGGGGAATCATTGCAACTTATAGTGGAGCTGGCTAT
  TATCTGGATTTGTCAAGAACAAGAGAGGAAACAGCTGCACAAGTTGCTAGCCTCAAGAAA
  AATGTCTGGCTGGACCGAGGAACCAGGGCAACTTTTATTGACTTCTCAGTGTACAACGCC
  AACATTAACCTGTTCTGTGTGGTCAGGTTATTGGTTGAATTCCCAGCAACAGGTGGTGTG
  ATTCCATCTTGGCAATTTCAGCCTTTAAAGCTGATCCGATATGTCACAACTTTTGATTTC
  TTCCTGGCAGCCTGTGAGATTATCTTTTGTTTCTTTATCTTTTACTATGTGGTGGAAGAG
  ATATTGGAAATTCGCATTCACAAACTACACTATTTCAGGAGTTTCTGGAATTGTCTGGAT
  GTTGTGATCGTTGTGCTGTCAGTGGTAGCTATAGGAATTAACATATACAGAACATCAAAT
  GTGGAGGTGCTACTACAGTTTCTGGAAGATCAAAATACTTTCCCCAACTTTGAGCATCTG
  GCATATTGGCAGATACAGTTCAACAATATAGCTGCTGTCACAGTATTTTTTGTCTGGATT
  AAGCTCTTCAAATTCATCAATTTTAACAGGACCATGAGCCAGCTCTCGACAACCATGTCT
  CGATGTGCCAAAGACCTGTTTGGCTTTGCTATTATGTTCTTCATTATTTTCCTAGCGTAT
  GCTCAGTTGGCATACCTTGTCTTTGGCACTCAGGTCGATGACTTCAGTACTTTCCAAGAG
  TGTATCTTCACTCAATTCCGTATCATTTTGGGCGATATCAACTTTGCAGAGATTGAGGAA
  GCTAATCGAGTTTTGGGACCAATTTATTTCACTACATTTGTGTTCTTTATGTTCTTCATT
  CTTTTGAATATGTTTTTGGCTATCATCAATGATACTTACTCTGAAGTGAAATCTGACTTG
  GCACAGCAGAAAGCTGAAATGGAACTCTCAGATCTTATCAGAAAGGGCTACCATAAAGCT
  TTGGTCAAACTAAAACTGAAAAAAAATACCGTGGATGACATTTCAGAGAGTCTGCGGCAA
  GGAGGAGGCAAGTTAAACTTTGACGAACTTCGACAAGATCTCAAAGGGAAGGGCCATACT
  GATGCAGAGATTGAGGCAATATTCACAAAGTACGACCAAGATGGAGACCAAGAACTGACC
  GAACATGAACATCAGCAGATGAGAGACGACTTGGAGAAAGAGAGGGAGGACCTGGATTTG
  GATCACAGTTCTTTACCACGTCCCATGAGCAGCCGAAGTTTCCCTCGAAGCCTGGATGAC
  TCTGAGGAGGATGACGATGAAGATAGCGGACATAGCTCCAGAAGGAGGGGAAGCATTTCT
  AGTGGCGTTTCTTACGAAGAGTTTCAAGTCCTGGTGAGACGAGTGGACCGGATGGAGCAT
  TCCATCGGCAGCATAGTGTCCAAGATTGACGCCGTGATCGTGAAGCTAGAGATTATGGAG
  CGAGCCAAACTGAAGAGGAGGGAGGTGCTGGGAAGGCTGTTGGATGGGGTGGCCGAGGAT
  GAAAGGCTGGGTCGTGACAGTGAAATCCATAGGGAACAGATGGAACGGCTAGTACGTGAA
  GAGTTGGAACGCTGGGAATCCGATGATGCAGCTTCCCAGATCAGTCATGGTTTAGGCACG
  CCAGTGGGACTAAATGGTCAACCTCGCCCCAGAAGCTCCCGCCCATCTTCCTCCCAATCT
  ACAGAAGGCATGGAAGGTGCAGGTGGAAATGGGAGTTCTAATGTCCACGTATGATATGTG
  TGTTTCAGTATGTGTGTTTCTAATAAGTGAGGAAGTGGCTGTCCTGAATTGCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_000297
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000297.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000297.2, NP_000288.1
• RefSeq Size: 5073 bp
• RefSeq ORF: 2907 bp
• Locus ID: 5311
• Cytogenetics: 4q22.1
• Domains: ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (1) represents the longer transcript.