LAMC2 (NM_005562) Human Untagged Clone

CAT#: SC315254

LAMC2 (untagged)-Human laminin, gamma 2 (LAMC2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAMC2
• Synonyms: B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005562, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGCGCTCTGGCTGGGCTGCTGCCTCTGCTTCTCGCTCCTCCTGCCCGCAGCCCGGGCCACCTCCA
  GGAGGGAAGTCTGTGATTGCAATGGGAAGTCCAGGCAGTGTATCTTTGATCGGGAACTTCACAGACAAAC
  TGGTAATGGATTCCGCTGCCTCAACTGCAATGACAACACTGATGGCATTCACTGCGAGAAGTGCAAGAAT
  GGCTTTTACCGGCACAGAGAAAGGGACCGCTGTTTGCCCTGCAATTGTAACTCCAAAGGTTCTCTTAGTG
  CTCGATGTGACAACTCCGGACGGTGCAGCTGTAAACCAGGTGTGACAGGAGCCAGATGCGACCGATGTCT
  GCCAGGCTTCCACATGCTCACGGATGCGGGGTGCACCCAAGACCAGAGACTGCTAGACTCCAAGTGTGAC
  TGTGACCCAGCTGGCATCGCAGGGCCCTGTGACGCGGGCCGCTGTGTCTGCAAGCCAGCTGTCACTGGAG
  AACGCTGTGATAGGTGTCGATCAGGTTACTATAATCTGGATGGGGGGAACCCTGAGGGCTGTACCCAGTG
  TTTCTGCTATGGGCATTCAGCCAGCTGCCGCAGCTCTGCAGAATACAGTGTCCATAAGATCACCTCTACC
  TTTCATCAAGATGTTGATGGCTGGAAGGCTGTCCAACGAAATGGGTCTCCTGCAAAGCTCCAATGGTCAC
  AGCGCCATCAAGATGTGTTTAGCTCAGCCCAACGACTAGACCCTGTCTATTTTGTGGCTCCTGCCAAATT
  TCTTGGGAATCAACAGGTGAGCTATGGTCAAAGCCTGTCCTTTGACTACCGTGTGGACAGAGGAGGCAGA
  CACCCATCTGCCCATGATGTGATTCTGGAAGGTGCTGGTCTACGGATCACAGCTCCCTTGATGCCACTTG
  GCAAGACACTGCCTTGTGGGCTCACCAAGACTTACACATTCAGGTTAAATGAGCATCCAAGCAATAATTG
  GAGCCCCCAGCTGAGTTACTTTGAGTATCGAAGGTTACTGCGGAATCTCACAGCCCTCCGCATCCGAGCT
  ACATATGGAGAATACAGTACTGGGTACATTGACAATGTGACCCTGATTTCAGCCCGCCCTGTCTCTGGAG
  CCCCAGCACCCTGGGTTGAACAGTGTATATGTCCTGTTGGGTACAAGGGGCAATTCTGCCAGGATTGTGC
  TTCTGGCTACAAGAGAGATTCAGCGAGACTGGGGCCTTTTGGCACCTGTATTCCTTGTAACTGTCAAGGG
  GGAGGGGCCTGTGATCCAGACACAGGAGATTGTTATTCAGGGGATGAGAATCCTGACATTGAGTGTGCTG
  ACTGCCCAATTGGTTTCTACAACGATCCGCACGACCCCCGCAGCTGCAAGCCATGTCCCTGTCATAACGG
  GTTCAGCTGCTCAGTGATGCCGGAGACGGAGGAGGTGGTGTGCAATAACTGCCCTCCCGGGGTCACCGGT
  GCCCGCTGTGAGCTCTGTGCTGATGGCTACTTTGGGGACCCCTTTGGTGAACATGGCCCAGTGAGGCCTT
  GTCAGCCCTGTCAATGCAACAACAATGTGGACCCCAGTGCCTCTGGGAATTGTGACCGGCTGACAGGCAG
  GTGTTTGAAGTGTATCCACAACACAGCCGGCATCTACTGCGACCAGTGCAAAGCAGGCTACTTCGGGGAC
  CCATTGGCTCCCAACCCAGCAGACAAGTGTCGAGCTTGCAACTGTAACCCCATGGGCTCAGAGCCTGTAG
  GATGTCGAAGTGATGGCACCTGTGTTTGCAAGCCAGGATTTGGTGGCCCCAACTGTGAGCATGGAGCATT
  CAGCTGTCCAGCTTGCTATAATCAAGTGAAGATTCAGATGGATCAGTTTATGCAGCAGCTTCAGAGAATG
  GAGGCCCTGATTTCAAAGGCTCAGGGTGGTGATGGAGTAGTACCTGATACAGAGCTGGAAGGCAGGATGC
  AGCAGGCTGAGCAGGCCCTTCAGGACATTCTGAGAGATGCCCAGATTTCAGAAGGTGCTAGCAGATCCCT
  TGGTCTCCAGTTGGCCAAGGTGAGGAGCCAAGAGAACAGCTACCAGAGCCGCCTGGATGACCTCAAGATG
  ACTGTGGAAAGAGTTCGGGCTCTGGGAAGTCAGTACCAGAACCGAGTTCGGGATACTCACAGGCTCATCA
  CTCAGATGCAGCTGAGCCTGGCAGAAAGTGAAGCTTCCTTGGGAAACACTAACATTCCTGCCTCAGACCA
  CTACGTGGGGCCAAATGGCTTTAAAAGTCTGGCTCAGGAGGCCACAAGATTAGCAGAAAGCCACGTTGAG
  TCAGCCAGTAACATGGAGCAACTGACAAGGGAAACTGAGGACTATTCCAAACAAGCCCTCTCACTGGTGC
  GCAAGGCCCTGCATGAAGGAGTCGGAAGCGGAAGCGGTAGCCCGGACGGTGCTGTGGTGCAAGGGCTTGT
  GGAAAAATTGGAGAAAACCAAGTCCCTGGCCCAGCAGTTGACAAGGGAGGCCACTCAAGCGGAAATTGAA
  GCAGATAGGTCTTATCAGCACAGTCTCCGCCTCCTGGATTCAGTGTCTCGGCTTCAGGGAGTCAGTGATC
  AGTCCTTTCAGGTGGAAGAAGCAAAGAGGATCAAACAAAAAGCGGATTCACTCTCAAGCCTGGTAACCAG
  GCATATGGATGAGTTCAAGCGTACACAGAAGAATCTGGGAAACTGGAAAGAAGAAGCACAGCAGCTCTTA
  CAGAATGGAAAAAGTGGGAGAGAGAAATCAGATCAGCTGCTTTCCCGTGCCAATCTTGCTAAAAGCAGAG
  CACAAGAAGCACTGAGTATGGGCAATGCCACTTTTTATGAAGTTGAGAGCATCCTTAAAAACCTCAGAGA
  GTTTGACCTGCAGGTGGACAACAGAAAAGCAGAAGCTGAAGAAGCCATGAAGAGACTCTCCTACATCAGC
  CAGAAGGTTTCAGATGCCAGTGACAAGACCCAGCAAGCAGAAAGAGCCCTGGGGAGCGCTGCTGCTGATG
  CACAGAGGGCAAAGAATGGGGCCGGGGAGGCCCTGGAAATCTCCAGTGAGATTGAACAGGAGATTGGGAG
  TCTGAACTTGGAAGCCAATGTGACAGCAGATGGAGCCTTGGCCATGGAAAAGGGACTGGCCTCTCTGAAG
  AGTGAGATGAGGGAAGTGGAAGGAGAGCTGGAAAGGAAGGAGCTGGAGTTTGACACGAATATGGATGCAG
  TACAGATGGTGATTACAGAAGCCCAGAAGGTTGATACCAGAGCCAAGAACGCTGGGGTTACAATCCAAGA
  CACACTCAACACATTAGACGGCCTCCTGCATCTGATGGACCAGCCTCTCAGTGTAGATGAAGAGGGGCTG
  GTCTTACTGGAGCAGAAGCTTTCCCGAGCCAAGACCCAGATCAACAGCCAACTGCGGCCCATGATGTCAG
  AGCTGGAAGAGAGGGCACGTCAGCAGAGGGGCCACCTCCATTTGCTGGAGACAAGCATAGATGGGATTCT
  GGCTGATGTGAAGAACTTGGAGAACATTAGGGACAACCTGCCCCCAGGCTGCTACAATACCCAGGCTCTT
  GAGCAACAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005562
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005562.2, NP_005553.2
• RefSeq Size: 5623 bp
• RefSeq ORF: 3582 bp
• Locus ID: 3918
• Cytogenetics: 1q25.3
• Domains: LamB, EGF_Lam
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two tr'
  Transcript Variant: This variant (1) represents the full length transcript variant. It encodes isoform (a) which is expressed in the epithelia of embryonic tissues. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments.