RASGRF1 (NM_002891) Human Untagged Clone

CAT#: SC315326

RASGRF1 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRF1
• Synonyms: CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002891, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCCCTGGGACTGCTGGCGCGCAAGGACGGCA
  CGCGCAAAGGCTACCTGAGCAAGCGGAGTTCGGACAACACAAAATGGCAAACCAAGTGGTTCGCGCTGCT
  GCAGAACCTGCTCTTCTACTTCGAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAGGGC
  TGCGTCTGCGACCGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAGCATT
  ACTTCACGGTGAACTTCAGCCATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGAGGACGCAAAAGATTG
  TGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACAGAGCATGAGGCATTAATG
  CAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAGACCGTGGCCAAGCAGCTTCGGCAGCAGA
  TCGAGGATGGGGAGATCGAGATCGAGCGGCTGAAGGCAGAGATCACATCCCTGCTCAAGGACAATGAGCG
  CATCCAGTCCACCCAGACTGTCGCCCCCAACGATGAAGACAGCGACATCAAGAAAATTAAGAAGGTGCAG
  AGCTTCCTGCGGGGCTGGCTGTGCCGGCGGAAGTGGAAGACCATCATCCAGGACTACATCCGGTCACCCC
  ATGCTGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGAGGCTGAGTACGTGCA
  GCAGCTGCACATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGATGGCCGCCAGCTCCAAGAAGCCTCCC
  ATCACACACGACGACGTCAGCAGCATCTTCCTGAACAGCGAAACCATCATGTTTTTACATCAGATCTTTT
  ACCAAGGCCTGAAGGCCCGCATCTCCAGCTGGCCCACGCTGGTCCTGGCTGACCTATTTGACATCCTGCT
  GCCCATGCTCAACATCTACCAAGAGTTCGTCCGCAACCACCAGTACAGCCTGCAGATCCTGGCCCACTGC
  AAGCAGAACCGTGACTTCGACAAGCTGCTGAAGCACTACGAGGCCAAGCCTGACTGCGAGGAGAGGACGC
  TGGAGACCTTCCTCACCTACCCCATGTTCCAGATCCCCAGGTACATCCTGACCCTCCATGAGCTCCTGGC
  CCACACGCCTCATGAGCACGTTGAGCGCAACAGCCTGGACTACGCCAAGTCCAAACTGGAGGAGCTGTCC
  AGAATAATGCACGATGAAGTAAGTGAGACGGAGAACATCCGGAAAAACCTGGCCATCGAGCGCATGATCA
  TCGAAGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTTGTGAGACAAGGTTCCCTCATTCAGGTGCC
  CATGTCTGAAAAGGGCAAGATCACCAGGGGGCGCCTGGGGTCTCTCTCCCTAAAGAAAGAGGGCGAGCGA
  CAGTGCTTCCTGTTTTCTAAGCATCTGATTATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACTTGACCA
  AGAATGGAGTCATATCCCTCATTGACTGCACTTTATTGGAGGAGCCAGAAAGCACGGAGGAGGAAGCCAA
  AGGATCCGGCCAAGACATAGATCACTTGGATTTTAAAATCGGGGTGGAGCCAAAGGATTCCCCGCCCTTT
  ACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAAGGCAGCGTGGACCAGTGACATCAGCCAGTGTGTGG
  ATAACATCCGATGCAATGGGCTCATGATGAACGCATTTGAAGAAAATTCCAAGGTCACTGTGCCGCAGAT
  GATCAAGAGGACCAGGGAGGGGACCAGGGAAGCAGAAATGAGCAGGTCCGACGCCTCCTTATATTGTGAT
  GATGTTGACATTCGCTTCAGCAAAACCATGAACTCCTGCAAAGTGCTGCAGATCCGCTACGCCAGTGTGG
  AGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTTCCTGAGCATCGACTTCCTCAACACCTTCCTGCACTC
  CTACCGCGTCTTCACCACCGCCATCGTGGTCCTGGACAAGCTCATTACCATCTACAAGAAGCCTATCAGT
  GCCATTCCTGCCAGGTGGCTGAGGTCGCTGGAGCTCCTGTTTGCCAGTGGCCAGAACAATAAGCTCCTGT
  ACGGTGAACCCCCCAAGTCCCCGCGCGCCACCCGCAAGTTCTCCTCGCCGCCACCTCTGTCCATCACCAA
  GACATCGTCACCGAGCCGCCGGCGGAAGCTCTCCCTGAACATCCCCATCATCACTGGCGGCAAGGCCCTG
  GACCTGGCCGCCCTCAGCTGCAACTCCAATGGCTACACCAGCATGTACTCGGCCATGTCACCCTTCAGCA
  AGGCCACGCTGGACACCAGCAAGCTCTATGTGTCCAGCAGCTTCACCAACAAGATTCCAGATGAGGGCGA
  TACGACCCCTGAGAAGCCCGAAGACCCTTCAGCGCTCAGCAAGCAGAGCTCAGAAGTCTCCATGAGAGAG
  GAGTCAGATATTGATCAAAACCAGAGTGATGATGGTGATACTGAAACATCACCAACTAAATCTCCAACAA
  CACCCAAATCAGTCAAAAACAAAAATTCTTCAGAGTTCCCACTCTTTTCCTATAACAATGGAGTCGTCAT
  GACCTCCTGTCGTGAACTGGACAATAACCGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCATAGCAACC
  GCCGGGGCCAACGAGGGCACCCCAAACAAGGAGAAGTACCGGAGGATGTCCTTAGCCAGTGCAGGGTTTC
  CCCCAGACCAGAGGAATGGAGACAAGGAGTTTGTGATCCGCAGAGCAGCCACCAATCGTGTCTTGAACGT
  GCTCCGCCACTGGGTGTCCAAGCACTCTCAGGACTTTGAGACCAACGATGAGCTCAAATGCAAGGTGATC
  GGCTTCCTGGAAGAAGTCATGCACGACCCGGAGCTCCTGACCCAGGAGCGGAAGGCTGCAGCCAACATCA
  TCAGGACTCTGACCCAGGAGGACCCAGGTGACAACCAGATCACGCTGGAGGAGATCACGCAGATGGCTGA
  AGGCGTGAAGGCTGAGCCCTTTGAAAACCACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCTGCTAGAT
  CACCTCGTCTTCAAGAAGATTCCTTATGAGGAGTTCTTCGGACAAGGATGGATGAAACTGGAAAAGAATG
  AAAGGACCCCTTATATCATGAAAACCACTAAGCACTTCAATGACATCAGTAACTTGATTGCTTCAGAAAT
  CATCCGCAATGAGGACATCAACGCCAGGGTGAGCGCCATCGAGAAGTGGGTGGCCGTAGCTGACATATGC
  CGCTGCCTCCACAACTACAATGCCGTACTGGAGATCACCTCGTCCATGAACCGCAGTGCAATCTTCCGGC
  TCAAAAAGACGTGGCTCAAAGTCTCTAAGCAGACTAAAGCTTTGATTGATAAGCTCCAAAAGCTTGTGTC
  ATCTGAGGGCAGATTTAAGAATCTCAGAGAAGCTCTGAAAAATTGTGACCCACCCTGTGTCCCTTACCTG
  GGGATGTACCTCACCGACCTGGCCTTCATCGAGGAGGGGACGCCCAATTACACGGAAGACGGCCTGGTCA
  ACTTCTCCAAGATGAGGATGATATCCCATATTATCCGAGAGATTCGCCAGTTTCAACAAACTGCCTACAA
  AATAGAGCACCAAGCAAAGGTAACGCAATATTTACTGGACCAATCTTTTGTAATGGATGAAGAAAGCCTC
  TACGAGTCTTCTCTCCGAATAGAACCAAAACTCCCCACCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002891
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002891.4, NP_002882.3
• RefSeq Size: 6414 bp
• RefSeq ORF: 3822 bp
• Locus ID: 5923
• Cytogenetics: 15q25.1
• Domains: IQ, RhoGEF, RasGEFN, PH, RasGEF
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.